Uhlalutyo lwe-HPLC, ukuchaneka kwedatha kubalulekile. Ukukhetha umthengisi othembekileyo kubalulekile kwiziphumo ezichanekileyo zohlalutyo. Nangona kunjalo, baninzi abathengisi emarikeni ukuba bakhethe. Ngaba uyazi ukuba ukhetha njani?

Kukho izinto ezintlanu eziphambili zokufumana umthengisi ofanelekileyo we-HPLC ye-autosampler kunye nezitrato.

Umgangatho wemveliso kunye nokuhambelana

Iqhosha le-HPLC le-HPLC kwimpumelelo ithatha ilungeloisampulu ye-vialnesixhobo. Zingatshatiswa ngokugqibeleleyo. Ke, kufuneka ufumane umthengisi. Kuya kufuneka banike uluhlu olubanzi lwezixhobo ze-vial kunye nobukhulu. Ezi kufuneka zihlangabezane neemfuno zakho zokuvavanya.

I-1. Izinto ze-Vial

Umzekelo, unokufuna iglasi. Okanye, unokufuna iplastiki. Kuxhomekeka kwiikhemikhali ozihlalutyeyo.

Iibhotile zeglasi zixhaphake kakhulu. Banobuqhetseba ngokugqwesileyo kunye nokuxhathisa imichiza. Yenye yezona zinto zisetyenziselwa kakhulu iilebhu. Ingasetyenziswa kwimimandla eyahlukeneyo.

Olunye ukhetho ziibhotile zeplastiki ezenziwepolypropleneokanye i-polylenene, ukhetho oluthe kratya kunye nexabiso eliphantsi. Yinto ekhethileyo yokhetho lweesampulu ezibuthathaka, isodium okanye uhlalutyo lwesinyithi esinzima. Kodwa ukuhambelana kwazo nezinyibilikisi okanye iisampulu zinokulinganiselwa.

I-2. Uyilo lwe-vial

Uyilo lwe-Vial kufuneka luhambelane ne-autosampler esetyenzisiweyo kwisixhobo sakho se-HPPC. Uqwalaselo loyilo oluphambili lubandakanya:

a. Ubume be-Vial kunye nobungakanani-kufuneka bathande ngokuthe ngqo kwitreyi ye-autosampler.

b. Ukugqiba kwentamo (intambo yokhukula, i-crimp,snap) -Ufanele ukuba uhambelane nenkqubo yokuvala.

c. Umthamo womthamo-Qinisekisa ivolumu ye-Vial ilungele ubungakanani besampulu yakho.

d. Ubukho bepatch okanye indawo yokwazisa isampulu.

e. Ezinye izinto ezikhethekileyo Vial zikwabandakanya iimpawu ezinje ngebhakhowudi, iSepta, okanye ifaka ukuphucula ukuhambelana nokulandelela.

Ufuna ukufunda ngakumbi malunga ne-Microfes? Eli nqaku liza kukunika intshayelelo ebanzi:Ukufakwa kwe-vial vial vial: jikelele ngokuchanekileyo kunye nokusebenza kakuhle kuhlalutyo lwe-HPPC

I-3. Isampulu yokuhambelana

Vavanya ngononophelo ukuba ngaba izinto ze-Vial ziya kuba ziyahambelana ne-matrix yakho ye-matrix. Izinto ekufuneka ziqwalaselwe:

a. I-polay kunye ne-pH yohlalutyo lwakho

b. Ubukho bezinyibilikisi zezinto eziphilayo, ii-asidi, okanye iziseko

c. Utyekelo lohlalutyo lwakho kwi-adkerb kwiglasi okanye kwindawo yeplastiki

d. Kuya kufuneka uvavanye i-vics kunye ne-matriki yokwenyani. Oku kuyakuqinisekisa ukuba akukho miba ifana nokukhuphela, i-adsorvo, okanye ukungqinelana.

Esi sixhobo kufuneka kwakhona sikwazi ukuxhomekeka kubushushu benkqubo yenkqubo ye-HPLC kunye noxinzelelo. Imelwe kukwenze oko ngaphandle kokuthambisa okanye izele. Jonga ukuba iimveliso zihlolwe kakuhle. Bavavanywa ngokungqinelana nesampulu, i-intetroness, kunye nokuzinza kwexesha elide. Oku kuqinisekisa ukuba abayi kulalanisa idatha yakho ngongcoliseko okanye i-adsorption.

LungiselelaI-HPLC VialsKwiisampulu zakho kunye nesixhobo sokuqinisekisa idatha ethembekileyo. Oku khetha i-vials exhasa ukuvela kwakhona kunye nengqibelelo.

Yintoni amandla emodeli yesampulu? Ungayifumana impendulo apha:Ubungakanani obu-7 obahlukeneyo bee-vialsgraphy zeChromatography kunye nokusetyenziswa kwazo

Umxhasi onamandla unokunika iingcebiso zengcali. Banokukunceda ukhethe i-vials efanelekileyo yokusetyenziswa okukhethekileyo.

Inkxaso yobugcisa ebanzi

Umboneleli onamava kufuneka anike inkxaso yobugcisa kwi-vials ye-HPLC kunye nezinto ezincedisayo. Benza le nto ukukunceda ufumane uninzi kwiimveliso zabo. Oku kubandakanya isikhokelo ngokukhetha uhlobo olufanelekileyo lwe-vial. Ikwabandakanya ukubaluleka kokuhambelana nesampulu ye-vial-vil. Kwaye, igubungela ukulungisa nayiphi na imicimbi onokudibana nayo. Jonga umthengisi one-smart, inkonzo yokuphendula iphinda iphendulwe. Banokulungisa imibuzo yakho kunye nenkxalabo yakho ngokukhawuleza. Benza le nto ngefowuni, i-imeyile, okanye ekutyeni kwisiza. Kuya kufuneka banike iingcebiso zengcali kwizinto. Oku kubandakanya imilinganiselo ye-Vial, iinkqubo zokuvalwa, kunye neempawu ezizodwa. Ingcebiso kufuneka ihambelane nezixhobo zakho ezithile ze-HPLC kunye neemfuno zesicelo.

Ukuthenjwa kweThankanya

Uhlalutyo lwe-HPPC luhlala lufuna ukutya. Oku kubandakanya i-vials,I-CAPS, neSepta. Vavanya ulawulo lweempahla zabathengisi, iinkqubo zokugcwalisa izalisekiswa, kunye nokuthenjwa kwentshukumo yoncedo. Ngaba banikezela ngeemveliso ngexesha lokuthintela ukulibaziseka komsebenzi? Cinga ngezinto. Oku kubandakanya amaxesha akhokelela. Kwakhona, uku-odola ukuchaneka kunye nokukwazi kwabo ukuphatha i-rush okanye kwimiyalelo yesiqhelo. Umthengisi olungileyo uya kuba nenethiwekhi yothumelo olomeleleyo. Baza kuba nempahla eguquguqukayo. Oku kuqinisekisa ukuba ufumana imali yakho ye-HLPL ngexesha, ngalo lonke ixesha.

Ulawulo loMgangatho kunye nokuthotyelwa komthetho

I-HPPC iqhelekile kwimizi-mveliso elawulwayo. Oku kubandakanya amayeza, ukutya kunye nokuvavanywa kwendalo. Ukunyaniseka kwedatha kunye nokuthotyelwa kubalulekile kula mashishini. Qinisekisa ukuba umboneleli ulawulo lomgangatho owomeleleyo. Oku kubandakanya ukuqinisekiswa kwe-ISO, uvavanyo lwe-LOT-lune, kunye namaxwebhu olawulo. Oku kukunika ukuzithemba. Iimveliso zabo ziya kuhlangana nomgangatho ophantsi kunye nemigangatho yolawulo. Oku kuyakukhusela ukuthembeka kweziphumo zovavanyo. Jonga abathengisi abanokubonelela ngezatifikethi zohlalutyo. Kuya kufuneka banikezele ngenkxaso yenkxaso kunye namanye amaxwebhu oqinisekiso lomgangatho. Oku kuyakubonisa ukuzibophelela kwabo kumgangatho wemveliso kunye nokuthobela.

Ufuna ukwazi ngakumbi malunga nolawulo lomgangatho, nceda ufunde eli nqaku:Ngaba uyakuqonda ukulawulwa komgangatho we-HPLC?

Iinkonzo ezongezwe Ixabiso

Umgangatho wemveliso kunye nokuthembeka kubalulekile. Kodwa, umthengisi we-HPPLC omkhulu kunye nempahla eninzi kufuneka anike iinkonzo ezongezelelweyo. Ezi nkonzo kufuneka ziphucule uhlalutyo lwakho. Jonga ababoneleli abanokwenza ngokwezifiso kwiimfuno zakho. Kuya kufuneka banike isikhokelo sophuhliso lweendlela zokuPhuhliswa kweMetroli okanye iinkqubo zolawulo lomthengisi ngokubanzi. Ezi zinto zangaphandle zinokuhambisa i-rolow yakho. Baya kwandisa nemveliso. Baza kuphucula ukubuyiselwa kutyalo-mali lwakho kwi-HLPC. Umzekelo, abanye abathengisi banokwenza ubungakanani bendalo. Banokwenza iintamo zesiko, ukuprinta, okanye ezinye izinto. Oku kusekwe kwiimfuno zakho. Abanye banokuba neenzululwazi zokusetyenziswa kwendlu. Banganceda ekuphumeleleni nasekuhlaleni.

Ngokuvavanya ezi zinto zi-5 ziphambili, unokufumana umthengisi. Bathengisa i-vials ye-autosampler kunye nezixhobo. Baza kuba liqabane lokwenyani ekuboneleleni ngedatha ethembekileyo, ekumgangatho ophezulu. Umboneleli othembekileyo uya kubonelela ngeemveliso ezintle. Baya kuyixhasa impumelelo yakho yelabhu. Bazakwenza oku ngobuchule. Banemixokelelwano yonikezo kunye nezinto ezintsha.

Zeziphi iimveliso eziphambili ze-Aijiren njengomthengisi we-chromatographys ezinokuthengiswa? Cofa apha ukuze ufunde ngakumbi ngathi:I-2ml yeglasi ye-vials yohlalutyo lwe-HPPC evela kwi-Aijiren

Ngaphambili:

Yonke into eyaziyo malunga ne-HPPC ye-vial ye-vial

Okulandelayo:

Ukutshisa ucoceko lwe-HPPLC: Isikhokelo esibanzi