Meyi. 20th, 2024
I-HPLC iyindlela yokuhlaziya ewusizo. Kusetshenziswa emikhakheni eminingi yesayensi. Phakathi kwalokhu kuyimithi, ukuhlaziya imvelo kanye nokuphepha kokudla. Baqamba inqubo yokuhlaziywa. Ngakho-ke, ukukhetha i-vial efanele kubalulekile. Kuthinta ukunemba nokwethembeka kwemiphumela.
HPLC VIALSGcina amasampula akho ephephile ekungcoleni nasemathonyeni angaphandle. Ungathola lezi zinhlamvu ezinhlotsheni eziningi, osayizi, kanye nezinto zokwakha. Bavumelana nezinhlobo ezahlukene zesampula kanye nezidingo zokuhlaziya. Uhlobo ngalunye lwe-HPLC VIAL lunikeza izinzuzo ezihlukile. Zinezici ezisiza ukuhlaziya kwakho ziphumelele.
Ama-HPLC Vavial anezinhloso ezimbili eziphambili. Bagcina amasampula ahlanzekile ngokuyeka ukungcoliswa futhi ezinzile. Ukungcola kungaba nezimbangela ezahlukahlukene. Isibonelo, impahla yebhodlela ingabaleka. Indawo yebhodlela ingaxhumana. Noma, i-vial ingaba nokungcola. Ukulungisa lezi zinkinga, abakhiqizi basebenzisa ingilazi esezingeni eliphakeme. Inokuqukethwe okuphezulu kwe-boron futhi ngokwenza i-HPLC isampula ama-vials. Bahlangabezana namazinga obuchwepheshe asemqoka. Lezi zindinganiso zimboza ububanzi obungaphakathi nangaphandle bomlomo nomzimba. Baphinde bamboze ukunemba komlomo onomuzwa. Bahlangabezana nazo zonke izidingo zamazinga aphesheya. I-batch ngayinye yomkhiqizo ithola ukulawulwa kwekhwalithi eqinile. Sinombiko wokuhlola efektri. Sinciphise ubungozi bokungcoliswa kwesampula.
Enye into esemqoka uma ihambisana ne-solves kanye nezigaba zeselula ekuhlaziyeni. Izinto ezihlukile ze-vial zinokumelana okuhlukile kwamakhemikhali kuma-solvents asolisayo noma amazinga okushisa aphezulu. Ukukhetha izinto ezifanele ze-vial zigcina i-vial kusuka ekuphumeni. Kuvimbela futhi ukulimala kwesampula noma ukungcoliswa.
Uhlelo lokuvalwa lwe-vial lubuye lubucayi ukugcina isampula liphephile. Ngokuvamile kwenziwa nge-PTFE kanye ne-silicone, ukuvimbela ukuhwamuka futhi kunciphise ubungozi bokungcola. Ukubekwa kahle okuhle kugcina isampula liqinile. Lokhu kubalulekile ngesikhathi sokuhlaziywa. Ikakhulu ngokuhamba isikhathi eside noma amakhompiyutha aguqukayo.
Isampula impahla ye-vials
Izinhlobo eziningi zesampula zenziwe ngengilazi. I-USP (United States pharmacopeia) ihlela ingilazi yelabhoratri ngokumelana kwayo kwamanzi.
1
I-BOROSILCE I-USP Type 1, ibanga A, 33 i-inert kakhulu futhi isetshenziswa kabanzi ezindaweni zokucwaninga, ikakhulukazi ku-chromatography, futhi iqukethe ngokuyinhloko i-silico ne-oxygen. Inesixazululo esiphansi kakhulu kanye nokwandiswa kokwenyuka okuqondile kwama-33.
2. Thayipha Ingilazi ye-BAROSILiLiLiling.
Le ngilazi ibizwa nge-borossilte ingilazi Usp Usp Uhlobo 1, isigaba B, 51. Kwenziwa kakhulu i-silicon nomoya-mpilo. Inamanani amancane e-boron, i-sodium, kanye nezinye izinsimbi ze-alkali ukwedlula ingilazi. Kepha, kusengasetshenziselwa ama-laboratories. Zonke izinhlobo ze-amber ziyizigaba ze-B
3. I-Polypropylene (PP)
I-PP iyipulasitiki engasebenzi. Inokumelana okuhle kwamakhemikhali. Ilungele ukugcinwa kwesikhashana kwamakhemikhali welebhu amaningi. Ingasetshenziswa lapho ingilazi kungeyona inketho. Lapho usebenzisa ama-hydrocarbons amnandi noma ama-halogenated, ukumelana kuncipha ngokuhamba kwesikhathi.I-PP VIALSisetshenziswa kabanzi e-Ion Chromatography. Lokhu kungenxa yokuthi banama-ion aphansi futhi bangahlanzwa ngamanzi abuthakathaka namanzi. Ama-polypropylene vials agcina izigqoko zawo emlilweni. Lokhu kunciphisa ukuvezwa kwezinto eziyingozi. Izinga lokushisa elisebenzayo lingu-135 ° C.
Ukukhethwa kwezimpukane zesampula ezenziwe ngezinto ezahlukahlukene
a. Ingilazi - isebenza kuyo emhlabeni wonke futhi i-acid;
b. I-Amber - yamasampula acwebezelayo;
c. I-Polypropylene - amasampula adakayo noma izixazululo ezingenamanzi;
Izinhlobo zama-vials asampula
Ama-sampula ama-vials ajwayele ukwahlukaniswa ngezinhlobo ezintathu. Lezi yilezi: crimp cap, isikulufa cap cap, kanye ne-snap ring cap vials.
Ungayikhetha kanjani phakathi kwama-vials amathathu asampula?
Kunezinhlobo ezintathu zama-cap atholakalayo we-Sampula Vers: CRIMP caps, Snap Caps, kanye nama-Screw Cap. Indlela ngayinye yokubekwa uphawu inezinzuzo zayo.
1. Crampimp top cap
I-crimp top cap inciphisa i-septum phakathi komugqa wesampula yesampula yengilazi ne-aluminium cap. Umphumela wokuvalwa uphawu muhle kakhulu futhi uvimbela ngempumelelo ukuhwamuka kwesampula. I-Septum ihlala endaweni. Inaliti ihlaba isampula. Ukuhlalisacrimp cap vialskudinga ukusetshenziswa kwe-capper. Ngenani elincane lamasampula, i-Manual Capper iyisinqumo esihle kunazo zonke. Ukuze uthole amasampula amaningi, i-capper ezenzakalelayo ingasetshenziswa.
2. Isikulufa ngentambo cap
Le khasiisikulufa ngentambokusemhlabeni wonke. Ukuqinisa i-cap kuncipha i-spacer. Kwenza lokho ngokufaka amandla okucindezela ngokumelene ne-rim yengilazi kanye ne-aluminium cap. Ngesikhathi sesampula, isikulufa cap seals kahle. Ibuye ibe yi-Septum. Awekho amathuluzi adingekayo ebandleni. I-screw cap ine-ptfe \
silicone Septum. Kunamathiselwe kwi-polypropylene cap usebenzisa inqubo engenalo-mahhala. Le techning tech igcina i-septum ne-cap ndawonye ngesikhathi sokuthumela. Iphinde ibagcine ndawonye lapho i-cap inamathele kwi-vial. Lesi sibopho sivimbela u-Septum ukuthi asondele ngesikhathi sokusebenzisa. Kepha, indlela esemqoka enamabala amandla lapho uqinisa i-cap ku-vial. I-cap iqinisa ukwenza uphawu. Ibuye ibe ne-septum endaweni ngesikhathi sokufakwa kwe-wite. Ungaqinisekisi i-cap cap ngokweqile. Kuzolimaza uphawu lwe-sep futhi kwenze i-septum iwe. Uma i-cap iqiniswa kakhulu, i-septum izokona noma ifakwe.
3. Snap Ring cap
Le khasiSnap capukwandiswa kwendlela yokubeka uphawu yesembozo somhlathi. I-cap yipulasitiki. Ifanelana noRim we-vial. Kuma uphawu ngokunciphisa i-septum phakathi kwengilazi kanye ne-cap cap yepulasitiki eyeluliwe. Ukungezwani ekhava yepulasitiki kubangelwa ukuzama kwalo ukubuyela kusayizi wayo wokuqala. Lokhu kungezwani kwakha uphawu phakathi kwengilazi, i-cap ne-septum. I-snap snap cap ingena ngaphandle kwamathuluzi.
a. Umphumela wokubopha uphawu we-snap cap awulungile njengezinye izindlela ezimbili zokubekwa uphawu.
b. Uma ukulingana kwe-cap kuqinile kakhulu, i-cap kuzoba nzima ukuyivala futhi ingahle iphule.
c. Uma kukhululeka kakhulu, uphawu ngeke lusebenze futhi septum lingaphuma endaweni.
Izimpawu ze-vial zingcono kunebhodlela. Irabha noma i-silicone iphinde yabeka kabusha kahle ngemuva kokubhoboza. Zilungele ukugcina ama-reagents.
Kodwa-ke, amanye ama-solvents azokonakalisa i-silica gel noma ama-gaskets enjoloba ngesikhathi sokusetshenziswa. Kulokhu, ama-vials adinga i-Septa nge-PTFE coating. Uma ngesikhathi sokusebenzisa, i-reagent ayisetshenziswa ngasikhathi sinye, kepha ingxenye yaso ishiywe ku-vial yesampula ngokuzayo. Uma usebenzisa isampula futhi, lokhu kwenzeka ngenxa yeSepta ebhobozekile ye-PTFE-eboshwe nge-PTFE elahlekelwe uphawu lwayo.
Irabha noma i-silicone vial setas iyamelana nezinye izinto. Amabhodlela amahle kakhulu ongawagcina kuwo yilawo aneziqongo ze-crimp. Ama-reagents awonakalisayo, nokho, ama-reagents awonakalisayo akufanele avalwe emabhodleleni abunjiwe. Irabha isebenza kangcono kune-silicone ngokuya ngokuvalwa kokubekwa uphawu.
Ngamafuphi, amabhodlela e-HPLC reagent abalulekile. Bagcina amasampula ahlanzekile futhi aqinisekise imiphumela efanele. Ukukhetha uhlobo lwebhodlela le-reagent elifanele, usayizi, kanye nokuqukethwe kungandisa imiphumela yokuhlaziywa kwakho kwe-HPLC. Kunganciphisa nengozi yamaphutha. Kungaba yi-vial ecacile yengilazi yokuhlaziywa okujwayelekile noma i-amber eyodwa yamasampula acwebezelayo. Noma, kungaba yinto ekhethekile ye-vial ekhethekile yevolumu noma i-pre-slit. Ama-vials alungile we-HPLC angasiza noma yimuphi umsebenzi wokuhlaziya.
IsiNgisi
-SiShayina
