Elabhorethri, i-Filtration idlala indima ebalulekile ezinhlelweni ezahlukahlukene zezicelo, kufaka phakathi ukulungiswa kwesampula, ukuhlanzwa, kanye nokubulawa inzalo. Izihlungi zesirinji ziwukuzikhethela okudumile ngenxa yokusebenziseka kalula, ukuguquguquka, kanye nekhono lokususa ngempumelelo udaba lokubandakanya kanye nama-microorganisms kusuka kumasampula. Phakathi kwezinhlobo ezahlukahlukene zokuhlunga okuyisirinji okutholakalayo, i-polyvinylidene fluoride (pvdf) izihlungi zesirinji ezihlole kakhulu ngenxa yezakhiwo zazo ezihlukile nezinqubo ezibanzi zezolabhoratri. Le ndatshana idonsela ekusetshenzisweni kweIzihlungi zesirinji ze-PVDFfuthi uhlole izinzuzo nokucatshangelwa kwazo.

I-PVDF Syringe Diplar Presories

I-PVDF i-hyrdloobic thermoplastic polymer enokumelana kahle kwamakhemikhali nokuqina. Lezi zakhiwo zenza izihlungi zesirinji ze-PVDF zilungele izinhlelo eziningi zezicelo, kufaka phakathi ukufunwa kwezinto ezinamanzi nama-organic sol. Izakhiwo ze-Hydrophobic ze-PVDF zivimbela ukumanzisa izihlunga, ukunciphisa ukulahleka kokuhlaziywa okubalulekile ngesikhathi sokuhlunga. Ngaphezu kwalokho, ulwelwesi lwe-PVDF lunokubopha okuphansi kwamaprotheni, okubaluleke kakhulu kwizicelo zezinto eziphilayo kanye ne-biochemical lapho ubumsulwa besampula bubaluleke khona. Ngaphezu kwalokho, izihlungi zesirinji ze-PVDF zinikeza amanani aphezulu okugeleza futhi akwazi ukumelana namazinga okushisa aphezulu, okuvumela inqubo yokuhlunga esebenzayo nesheshayo.

4 Izicelo ikakhulukazi

Inzalo kanye nokucaciswa

Izihlungi zesirinji ze-PVDFzivame ukusetshenziselwa inzalo kanye nokucaciswa kwamasampula welebhu. Zilungele ukuhlunga imidiya yesiko leselula, ama-buffers, kanye nama-reagents ngoba asuse ngempumelelo amagciwane, imvubelo, namanye amagciwane. Izihlungi zesirinji ze-PVDF zingabanjiswa ngokuzenzakalela noma i-gamma invadadiated ukuqinisekisa ukuqina kwezixazululo ezihlungiwe. Lokhu kubenza babaluleke kakhulu ekugcineni ubuqotho nokuphepha kwamasampula nezisombululo.

Vula imininingwane yokuhlunga okungu-0.45 micron kule ndaba ephelele. Uma unesifiso sokufunda ngokusetshenziswa kwazo nezinzuzo, ungaphuthelwa ukuyihlola:Igayidi ephelele yokuhlunga okungu-0.45 micron: konke okudingeka ukwazi

I-HPLC Sampula Ukulungiselela

I-Chromatography ephezulu yokusebenza kwe-chromatography (HPLC) isebenzisa izihlungi ukususa udaba lokushaya kanye nokuvimbela ukuvalwa kwekholomu yokuhlaziya. Izihlungi zesirinji ze-PVDF ezinosayizi we-pore of 0.2 μm noma u-0.45 μm zisetshenziselwa ukulungiswa kwesampula ye-HPLC. I-hydrophobic yemvelo ye-PVDF ivimbela isihlungi ekutholeni kwamanzi noma ibe manzi, incishise ukulahleka kokuhlaziywa okubalulekile ngesikhathi sokuhlunga. Lokhu kuqinisekisa imiphumela ethembekile futhi engaguquki ekuhlaziyweni kwe-HPLC.

Ukuhlunga kwe-solvent okunolaka

Izihlungi zesirinji ze-PVDF zimelana kakhulu nezinhlobonhlobo ze-solvents, kufaka phakathi ama-acid, izisekelo, kanye nezinhlelo ze-organic. Le mpahla ibenze balungele ukugcwala kwezinto ezinolaka ezisetshenziswe kumakhemikhali wokuhlaziya, njengezixazululo zesigaba seselula ngesigaba se-chromatography esibuyiselwe emuva. Izihlungi zesirinji ze-PVDF zisusa ngempumelelo ama-nincoulates, ukuvikela amasistimu we-chromatographic kusuka kumonakalo, futhi uqinisekise imiphumela yokuhlaziya ethembekile.

Ukuhlaziywa Kwezemvelo

Ukuhlaziywa kwezemvelo kuvame ukufaka ama-matricis ayinkimbinkimbi namasampula aqukethe udaba lokubandakanya okungaphazamisa ukuhlukunyezwa kokuhlaziya. Izihlungi zesirinji ze-PVDF ezinamasayizi ahlukahlukene e-pore zingasetshenziswa ukususa ama-concleates kanye nokuhlanza amasampula emvelo anjengamanzi, okukhishwa inhlabathi, kanye nezinhlaziyi zomoya. Lokhu kuvumela ukuhlaziya okunembile nokuthembekile kokungcola, ukungcola, kanye nokunye ukuhlaziya okuhlosiwe.Izihlungi zesirinji ze-PVDFDlala indima ebalulekile ekutholeni idatha enembile nenenjongo yokuqapha imvelo kanye nokuhlaziywa.

Thola iqiniso ngokusebenzisa izihlungi zesirinji. Ingabe izihlungi zesirinji zingasetshenziswa kabusha? Thola kule ndaba efundwayo ngesihloko sokuphinda usebenzise isihlungi se-syringe:Ukuhlunga ngezimpawu uzokusebenzisa futhi?

Okufanele ucabangele

Ukuphathakwa

Izihlungi zesirinji ze-PVDF ziyahambisana nezinhlangothi eziningi ze-solvents kanye nezixazululo, kepha kubalulekile ukuqinisekisa ukuhambisana ngokubhekisisa ezincomeni zomkhiqizi noma ukwenza izivivinyo zokuhambisana.Izinto ezihlanganisiwe ze-organ. Ukuqinisekisa ukuhambisana kubalulekile ukuze kugcinwe ubuqotho besampula futhi kuvimbele umonakalo ongaba khona wokuhlunga.

Amaprotheni abophayo

Yize ulwelwesi lwe-PVDF lunezindawo eziphansi zokubopha amaprotheni, amanye amaprotheni angabopha indawo yokuhlunga.Ukunciphisa ukugeza i-protein advent, kunconyelwa ukugeza okuphathelene nokulahleka kwamaprotheni futhi kusiza ekuqinisekiseni ukuhlaziya okunembile kwe-townstream kuzicelo zemvelo kanye ne-biochemical.

Ingcindezi nokugeleza kwezinga

Izihlungi zesirinji ze-PVDF ngokuvamile zinokumelana okuphansi kokugeleza, okuvumela ukuhlunga okusheshayo. Kodwa-ke, ingcindezi eyeqile ingaholela ekuhlungeleni kokuhlunga nokuvalwa ngaphambi kwesikhathi. Ukuthola imiphumela ethembekile futhi engaguquki, kubalulekile ukunamathela ekucindezelweni okukhulu okutukwe futhi uqinisekise izimo ze-FILTRATION ezifanele.Proper kanye nokulawula ukuhamba nokulawula ukugeleza kubalulekile ukuze ulondoloze ubuqotho kanye nokusebenza kahle kwenqubo yokuhlunga.

Izihlungi zesirinji ze-PVDFNikeza ngesisombululo esiguquguqukayo kwinqubo yokuhlunga yelabhoratri ngokusebenzisa ukumelana kwamakhemikhali okuhle, ukubopha okuphansi kwamaprotheni, kanye nokumelana okushisa okuphezulu. Izicelo ziqala ukubulawa inzalo kanye nokucaciswa, ukulungiswa kwesampula kwesampula ye-HPLC, ukuhlunga kwe-solvent okunolaka, kanye nokuhlaziywa kwemvelo. Ukuqonda izakhiwo ezihlukile kanye nokucatshangelwa okuhambisana nokuhlunga kwesirinji kwe-PVDF kuvumela ososayensi ukuba benze izinqumo ezinolwazi futhi bazuze imiphumela ethembekile futhi enembile ezinhlelweni zokuhlunga zelebhu. Ukufaka izihlungi zesirinji ze-PVDF ekuhambeni kwelabhoratri kuthuthukisa ukusebenza kahle, ukunemba, kanye nobuqotho besampula, okufaka isandla ekucwaningeni nasekuphumeleleni kokuhlaziya.

Thola i-ins kanye nokuphuma kokuhlunga kwe-micron engu-0.22 kulesi sihloko esifundisayo. Uma ufuna ukwazi ngezicelo zabo nezinzuzo, qiniseka ukuthi uyihlola:Igayidi ephelele yokuhlunga okungu-0.22 micron: konke okudingeka ukwazi

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