Ungayikhetha kanjani i-HPLC elungile ye-HPLC ye-sople yakho?

I-Chromatography ephezulu yokusebenza i-chromatography (HPLC) iyindlela ebalulekile yokuhlaziya ehlaziya esetshenziswa emikhakheni ehlukahlukene efana nemithi, ukuhlaziya imvelo, nokuphepha kokudla. Ingxenye esemqoka yohlelo lwe-HPLC yi-septum ye-vial septum, esebenza njengokuvalwa kwe-vial. I-septum ye-vial ukuvalwa okuvimbela ukuvulwa kwe-vial, okuvumela ukungena kwenaliti ngenkathi kugcinwa uphawu oluvikelekile ukuvikela ukungcoliswa nokuhwamuka. Kufanele zihambisane ne-solvent ehlaziywa futhi kufanele zikwazi ukumelana nemibandela yenqubo ye-HPLC.

Ukubaluleka kokukhetha i-Septa efanele

Ukukhetha i-Septum efanele kubalulekile ku:

Ukuvimbela ukungcoliswa: Izinto ezingahambelani zikwazi ukubona isampula noma usabele nge-solvent.

Ukuqinisekisa uphawu olufanele: Isingeniso esihle kuvimbela ukulahleka kwesampula futhi kugcina ubuqotho.

Ukwenza lula imijovo eminingi: Ezinye ze-Septa zenzelwe ukusebenza ngaphandle kokuwohloka.

Ufuna ukwazi ulwazi oluphelele mayelana ne-PTFE \ abicone septa, sicela uhlole le ndatshana: Konke odinga ukukwazi: 137 pre-slit ptfe \ airtone septa FAQs

I-HPLC VIAL Septa Izinhlobo zezinto

Izinto ezahlukahlukene zinezakhiwo ezihlukile ezithinta ukusebenza kwazo kuzinhlelo zokusebenza ze-HPLC. Okulandelayo izinto ezisetshenziswayo ezisetshenziswa kakhulu ku-HPLC VIAL Septa:

1. I-PTFE (polytetrafluoroethylene)

Izici: I-PTFE inokumelana kahle kwamakhemikhali, i-inert kakhulu futhi ingamelana nezixazululo ezinolaka.

Isebenzisa: Ayilungele imijovo eminingi noma isitoreji eside ngenxa yokungakwazi kwayo ukukopela ngemuva kokubhoboza, okwenza kube kuhle kakhulu kwizicelo ezisetshenziswayo.

Ukumelana nokushisa okuphezulu: ukumelana namazinga okushisa aze afike ku-260 ° C.

Izindleko: Ngokuvamile kwezomnotho kuqhathaniswa nezinye izinto.

2. I-PTFE \ silicone Septa

Izici: Kuhlanganisa ukumelana kwamakhemikhali kwe-PTFE ngokulungiswa kwe-silicone.

Sebenzisa: Kunconyelwe imijovo eminingi kanye nesitoreji sesampula ngenxa yokusekela kwayo okuhle kakhulu.

Ububanzi bokushisa: busebenza kusuka ku-40 ° C kuya ku-200 ° C.

I-Autoclable: Ilungele izinqubo zokubulala inzalo.

3. Pre-cut ptfe \ abicone septa

Izici: Idizayinelwe ukunciphisa ama-coring lapho usebenzisa izinaliti ezinama-blunt; Yehlisa ukwakheka kwe-vacuum kuma-vials.

Isebenzisa: Ilungele izinhlelo zokusebenza lapho kudingeka khona oxhumana naye enaliningi ngaphandle kokuyekethisa ubuqotho be-Septum.

Ububanzi bokushisa: okufana ne-PTFE \ silicone, -40 ° C kuya ku-200 ° C.

4. I-Butyl Rubber

Izakhiwo: Izakhiwo ezinhle zokubopha uphawu, kepha ukumelana okulinganiselwe kwamakhemikhali kuqhathaniswa ne-PTFE.

Isebenzisa: Ilungele izixazululo zamanzi nama-solvents amancane anolaka; Ingasetshenziswa kuzinhlelo zokusebenza ezidinga uphawu lwe-Airtight.

Ukumelana nokushisa okuphezulu: Okuhle emazingeni okushisa aphansi, kepha kungahle kungcolise amazinga okushisa aphezulu noma ngezinto ezinolaka.

5. Iviyo

Izakhiwo: Ukumelana kakhulu namakhemikhali anolaka futhi kugcinwe amandla athile okusola ngemuva kokubhoboza.

Ukusetshenziswa: kubhekwe njengendawo yokugcina ngenxa yezindleko eziphakeme; Ilungele izinhlelo zokusebenza lapho ezinye izinto zehlulekile.

Ukumelana nokushisa okuphezulu: Kuyasebenza emazingeni okushisa aphezulu, kepha kudinga ukuphatha ngokucophelela ngenxa yokuqina kwayo.

Izici okufanele zicatshangelwe lapho ukhetha i-HPLC VIAL Septa

1. Ukuvumelana Kwamakhemikhali

Into yokuqala okufanele uyicabangele ukuhambisana kwamakhemikhali kwezinto ze-septum ezinama-solvents ohlela ukuzisebenzisa. Izinto ezahlukahlukene zinokubekezelela okuhlukile kumakhemikhali:

I-PTFE (polytetrafluoroethylene): I-PTFE yaziwa ngokumelana kwayo okuhle kwamakhemikhali futhi ilungele inqwaba yezinhlanzi, kufaka phakathi amakhemikhali awonakalisayo. Imvamisa isetshenziswa ngokuhlanganiswa nezinye izinto.

I-Silicone: I-Silicone Septa iyavumelana nezimo futhi ingabhekwa kabusha ngemuva kokungena nge-wirege, ibenze balungele imijovo ephindaphindwayo. Kodwa-ke, kungenzeka zingahambelani nawo wonke ama-solvents.

I-Buyl Rubber: Lokhu okuqukethwe kunezakhiwo ezinhle zokubopha uphawu, kepha kunokumelana okulinganiselwe kwamakhemikhali kuqhathaniswa ne-PTFE.

I-PTFE \ Silicone Composite: Kuhlanganisa izinzuzo zazo zombili izinto zokwakha, ngokuhambisana okuhle kwamakhemikhali nokusetshenziswa kabusha.

2. Uhlobo lwe-solvent

Uhlobo lwe-solvent olusetshenzisiwe luzothonya nokukhethwa kwe-septum:

Izisombululo zamanzi: ama-solvents asuselwa emanzini, i-silicone noma i-ptfe \ silicone Septa zivame ukunconywa ngenxa yokuhambisana kwawo okuhle.

I-Organic Solvents: Ngezinhlelo ze-organic ezifana ne-methanol noma i-acetonitritrile, i-PTFE noma i-PTFE \ ama-silicone composites ancanyelwa ukumelana kwawo kwamakhemikhali.

Amakhemikhali awonakalisayo: Kumakhemikhali awonakalisayo awonakalisayo anjengama-acid aqinile noma izisekelo, i-PTFE imvamisa iyisinqumo esihle kakhulu.

3. Ukusebenza uphawu

Amandla we-Septum wokuphinda akopele ngemuva kokubhoboza inaliti kubalulekile ekugcineni ubuqotho besampula:

I-Slit vs Design Engelona-slit: Ezinye ze-SEPTA ziye zivimbela ukufakwa kwenaliti, kepha lokhu kungathinta uphawu ngokuhamba kwesikhathi. Imiklamo engeyona eye-slit inikezela nge-tighter seal kepha ingadinga amandla amaningi lapho ijova.

Ukusetshenziswa kabusha: Funa nge-Septa ekwazi ukuphindisela ngempumelelo ngemuva kwama-punsle ama-Pinali amaningi ukuvikela ukungcoliswa nokuhwamuka.

Ufuna ukwazi ukuthi ukhetha iSepta ku-Pre-Slit noma cha? Sicela ubheke le ndatshana: Ungayikhetha kanjani i-Septa Preta Pre-Slit noma cha?

4

Lapho usebenza ngamasampula abucayi, kubalulekile ukuthi ubheke ukukhishwa kwangaphandle kanye nokukhafulela ngezinto ezibonakalayo ze-Septum:

Ama-extractable aphansi: Khetha Septa nge-extractables ephansi ukunciphisa ubungozi bokungcola. I-PTFE inezinto ezikhishwe eziphansi uma ziqhathaniswa nezinto zokwakha ezisekelwa irabha.

Amazinga wokuhlola: Abanye abakhiqizi bahlinzeka ngemininingwane yokukhishwa; Buyekeza lezi zincwadi lapho ukhetha uSeptum.

I-5. Ukumelana okuphezulu okuphezulu

Cabanga ngobubanzi bokushisa lapho uhlelo lwakho lwe-HPLC luzosebenza khona:

Ukushisa okuphezulu: Uma uhlelo lwakho lokusebenza lubandakanya amazinga okushisa aphezulu, qiniseka ukuthi i-Septum Material ingamelana nalezi zimo ngaphandle kokonakaliswa.

Izicelo ze-Cryogenic: Okwezinhlelo zokusebenza ze-CryOgenic, khetha izinto ezigcina ubuqotho emazingeni okushisa aphansi.

6. Izakhiwo ezibonakalayo

Izici zomzimba ze-Secum zingathinta futhi ukusebenza:

Ubukhulu: I-Swer Septa inganikeza uphawu olungcono kodwa ingadinga amandla amaningi ngesikhathi sokungena kwenaliti.

Ubulukhuni: Izinto ezithambile zinganikeza ukuphelelwa okungcono kepha kungahle kube nokulimala ngokwengeziwe ngesikhathi sokuphindaphinda okuphindaphindiwe.

Ukukhetha i-HPLC elungile ye-HPLC ye-Senial Septum kudinga ukucubungula ngokucophelela ukuhambisana kwe-solvent, amakhono okubekwa uphawu, ukumelana nokushisa okuphezulu, kanye nezidingo ezithile zokuhlaziya. Ngokuqonda izakhiwo zezinto zokwakha ezahlukahlukene kanye nezicelo zazo, abacwaningi bangathuthukisa kakhulu ukunemba nokuthembela kokuhlaziywa kwe-HPLC. Ukukhetha okulungile akugcini nje ngokuthuthukisa ukusebenza, kepha futhi kunciphisa ubungozi obuhambisana nokuwohloka kwesampula nokungcola.