Ama-Autosamplers adlala indima ebalulekile kwamanye ama-latoratorics wanamuhla wokuhlaziya ngokushintsha inqubo yesampula yezethulo nokunikeza uhlelo oluzenzakalelayo lweSampula. Isici esibalulekile salokhu ukulayisha okufanele kwamasampula kuama-autosampler vialskanye nokulungiswa kwesampula okusebenzayo; Yomibili imisebenzi zinokuqanjwa kabusha okuphambili kwekhwalithi nokwethenjwa kwemiphumela kusuka ekuhlaziyweni. Sethula lapha izinyathelo ezibalulekile, imikhuba emihle kakhulu, namasu wokuqinisekisa ukusebenza kahle ngesikhathiUkulungiswa kwesampula\ izinqubo zokulayisha ze-vial ezinama-autosamplers.

Isigaba 1: Ukulungiselela ukulayisha amasampula

Ngaphambi kokuqala ukulayisha isampula, kubalulekile ukuthi udale izimo ezinhle. Nakhu okudingeka kwenzeke:

1.1 Hlanganisa imishini yakho

Qala ngokubutha yonke imishini edingekayo nezinto zokwakha:

Ama-autosampler vials: Khetha ama-vials ahambisana nohlobo lwakho lwe-autosampler nohlobo lokuhlaziya - Ama-autosamplers amaningi asebenzisa ingilazi noma ama-vials epulasitiki walo msebenzi.

I-CAPS ne-Septa: Ngokuvikelwa okuphezulu ekungcolisweni nasekuhanjisweni, kuqinisekisa ama-cap-cap afanelekile kanye ne-septa evumelana ngokuqinile ukuvikela ukuvuza nokungcola.

Amathuluzi wesampula wesampula: Zilungiselele ngamapayipi, amasirinji nezinye izingcezu ezidingekayo zemishini uma kukhulunywa ngesampula prep. Lokhu kungafaka ama-Pipette, ama-syringe kanye nemishini yokuhlazeka uma kusebenza.

Gcina indawo yokuhlanza ukuze yehlise ubungozi bokungcola.

1.2 Faka ilebula ama-vials akho

Ngaphambi kokuhlaziywa kokuqala, i-vial ngayinye kufanele ibhalwe ngokucacile ngolwazi oluyinhloko kufaka phakathi ukukhonjwa kwesampula, usuku kanye neminye imininingwane efanelekile. Ukulebula kuqinisekisa ukulandelela kanye nokuvimbela ama-mix-ups ngesikhathi sokuhlaziywa.

1.3.handle Samples ngokunakekela

Phatha ngokucophelela amasampula ukuze ugweme ukungcoliswa nokulahleka. Sebenzisa amathuluzi afanele, njengama-Pipette noma ama-syringe, lapho kudlulisela amasampula ezihlotsheni; Gwema ukuthinta ama-Interiors noma ama-cap of vials ngeminwe njengoba lokhu kungenisa amafutha esikhumba kanye nokungcola okungaguqula imiphumela.

1.4.Qinisekisa ivolumu yesampula enembile

IVolumu Enembile yesampula ibalulekile ekuzuzeni imiphumela ethembekile. Sebenzisa imishini evuthayo ukukala ngokunembile futhi udlulise ivolumu efanelekile kuma-vials; Njalo gcina ngokucaciswa kwevolumu yevolumu yakho ye-Analytical Hamplex.

1.5 Nciphisa ama-bubble omoya

Ama-Bubble amoya angenza umjovo wesampula unembe kakhudlwana; ukunciphisa ukwakheka kwabo:

Gwema amasampula axosha ngamandla kwi-vial. Gcwalisa kancane futhi kahle, ngenkathi uthepha ngobumnene ukuze ukhiphe ama-bubble omoya abhajwe.

Lapho amasampula alayishwa ngama-vials, abeke uphawu ngokuphepha usebenzisa izigqoko ezifanele kanye ne-septa. Ukufezekisa uphawu lwe-airtitight kuzosiza ukuvikela ukuhwamuka kwesampula noma ukungcoliswa; Lapho usebenzisa ama-crimp caps esebenzisa ithuluzi ngokukhethekile ngalo msebenzi wokuvalwa okuphephile.

Hlola ukuqonda okujulile ku-Insights ku-HPLC Sampula Ukulungiselela kule Article Article:I-HPLC isampula izixazululo zokulungiselela imiphumela emihle kakhulu

Isigaba 2: Amasu wesampula wesampula

Ukulungiswa kwesampula okusebenzayo kuvame ukuba kubalulekile ekuhlaziyweni okunembile. Ngokuya ngendlela yakho yokuhlaziya, amasampula angadinga ukuhlunga, ahlanjululwe noma aphathwe ngenye indlela ngaphambi kokulayisha ama-vials autosampler - qiniseka ukulandela noma yiziphi izindlela ezinconyelwe zokulungiselela amasampula ukuze le nqubo.

Isigaba 3: Ukulayisha amasampula ku-autosampler vials

Manje njengoba amasampula akho alungisiwe, sekuyisikhathi sokuwalayisha ngokuhlelekile kuma-autosampler vials:

3.1 gcina ama-vials kahle

Uma ukuhlaziya kwakho kudinga isikhathi sokulinda esandisiwe ngaphambi kokujova, qiniseka ukuthi ama-vials agcinwe ngaphansi kwezimo ezifanele - isiqandisini noma ukuvikelwa kokukhanya kwe-UV kungahle kudingeke ngokuya ngohlobo lwesampula oluphelele.

3.2 Hlela ama-vials akho

Sungula uhlelo olusebenzayo lokuhlela ama-vials akho. Hlela ngokunengqondo, futhi ubheke ukusebenzisa ama-racks avida noma amathreyi ukugwema ukuxubana ngesikhathi sokuhlaziywa.

3.3 Yenza isheke lokufaneleka kohlelo

Ngaphambi kokuthi kusebenze amasampula, kungakuhle ukuthi wenze isheke lokufaneleka kohlelo. Lokhu kuqinisekisa ukuthi i-autosampler isebenza njengoba ihlosiwe nokuthi amasampula akho alayishwe ngokunembile kukho.

3.4 Ithuluzi lokuhlola lokuhambisana lokuhambisana (VCT)

Amasampula ahlukene adinga izipesheli eziqondile; amber nomaSula ama-vialsKwangathi kwanele ngenkathi ama-vials akhethekile enzelwe ngqo izinhlelo zokusebenza ezithile nazo zingadingeka. Kuwukuhlakanipha ukuqinisekisa ukuhambisana kwama-vials akho ngohlobo lwesampula zombili kanye nendlela yokuhlaziya ukuze ugweme izindaba ezingaba khona ngesikhathi sokuhlaziywa.

Isigaba 4: Ukulayishwa kwangemva kokuhlaziywa nokuhlaziywa

4.1 Gcina futhi uhlanze izakhi ze-autosampler

Ukuze ulondoloze ukusebenza okungaguquki kwe-autosampler, ukulondolozela njalo nokuhlanza izingxenye zalo - kufaka ama-Syringes ama-Promos, ama-syringe, kanye namachweba okujova. Okusele noma okungcolile kungahle kube nomthelela odlulayo ngokudluliswa kwesampula okuholela emiphumeleni engalungile; Ukulandela izincomo zomkhiqizi bese usungula uhlelo lokulondolozwa.

4.2 Bhala inqubo yakho

Isici esibalulekile somsebenzi welebhu sigcina imibhalo ephelele. Qiniseka ukuthi ufaka noma yikuphi ukuphambuka noma izingqinamba ezihlangabezana nazo ngesikhathi sokulayisha isampula kulo mbhalo, ozosebenza njengomthombo obalulekile wokuxazulula inkinga nokulandela ukuxazulula inkinga nokulandela umsebenzi wokuhlaziya umsebenzi.

4.3 Amasheke okuqinisekisa ajwayelekile ajwayelekile kufanele aqalwe

Sebenzisa uhlelo olusebenzayo lwekhwalithi (QC) ukuhlola ukusebenza kwe-autosampler nokubuya, okungafaka phakathi amazinga akwaziwayo, izikhala kanye nemininingwane yokuhlola ukunemba nokunemba. Noma yikuphi ukuphambuka kusuka emiphumeleni elilindelekile kufanele kusheshe kusheshe kusheshe futhi kuthathwe izinyathelo zokulungisa ngokushesha.

4.4 Xazulula ngokushesha izingqinamba

Ukuxazulula izinkinga ezilindelwe noma imiphumela engahambisani ngokushesha kubalulekile uma kuvela, njengezinkinga zokulayisha ezingalindelekile ezinjengokungcola noma ukungcoliswa kwehlisa ikhwalithi yedatha. Ngokuthatha indlela ehlelekile yokuxazulula inkinga, izinkinga zizokhonjwa kalula futhi zilungiswe ngokushesha.

Ukwenza kahle ubuciko bokulungiselela amasampula nokulayisha ama-vials we-autosampler kuyisihluthulelo sokukhiqiza imiphumela yokuhlaziya ethembekile. Ngokunamathela emikhubeni engcono kakhulu, ukugcina imishini, nokubheka izingqinamba ezingaba khona, ungaqinisekisa ukuthi amasampula akho alayishwa ngokunembile futhi ngokungaguquki - into ezonikela kakhulu ekuqinisekiseni impumelelo ephelele yomsebenzi wakho wokuhlaziya - ukuhlinzeka ngemininingwane ethembekile yezinhloso zokuhlola noma zekhwalithi.

Thola izimpendulo zemibuzo engama-50 ejwayelekile ye-HPLC kule ndaba ephelele futhi efundisayo:Imibuzo evame ukubuzwa kaningi ku-HPLC VIALS

Okwedlule:

Izizathu ezi-13 eziphezulu zokusebenzisana nenkampani yethu yezihambi ze-Chromatography

Olandelayo:

Yini i-vial ephakeme yokutakula?