Ukukhetha ama-vials afanele we-autosampler kubalulekile. Ukuzisebenzisa kahle, futhi. Banquma ukuthi ukuhlaziya okunokwethenjelwa futhi okunembile kwe-HPLC kuyinto ethembekile. Ivolumu ye-vial ingathinta ubuqotho besampula. Kuthinta nokunemba kwemininingwane. Lo mbhalo uhlola amasu wokwenza kahle ivolumu ye-HPLC autosampler vial. Umgomo ukusika ukulahleka kwesampula futhi ugcine ukuhlaziya okunembile.

Ukuqonda ivolumu evamile

Kunosayizi ojwayelekile ojwayelekile we-HPLC autosampler vials etholakalayo, njenge-1.5-2 ml, 4 ml, kanye ne-150-300 μl ukufakwa okuncane. Esetshenziswa kakhulu futhi ekwazi ukuhlangabezana nezidingo zokuhlola kwansuku zonke yilezi zinto eziyi-1.5-2 ml isampula yesampula. Lapho amasampula adinga amavolumu akhulu kune-3 ml, sisebenzisa iziqukathi ezinkulu ezi-4 ml. Lezi zinhlaka ezinkulu zingabamba amancane zifaka ngaphakathi kwazo. Lokhu kusetshenziselwa amavolumu amancanyana amakhulu, phakathi kuka-150 no-300 μL.

Ukwazi izimfanelo nokusetshenziswa okufanele kwalawa masayizi we-HPLC autosampler vial ukhiye. Ukukhetha usayizi we-vial ofanele kubalulekile. Kufanele ifane nevolumu yesampula. Lokhu kusiza ukunciphisa ukulahleka kwesampula futhi kugcinwe isampula ingenakuhlaziywa ngesikhathi sokuhlaziywa.

Funa ukwazi kabanzi nge-HPLC ye-Vial Exeptions, sicela uhlole le ndatshana I-HPLC Vial Exernts: Ukuthuthukisa Ukunemba Nobuqotho Besampula

Ungayikhetha kanjani ngokulandela isampula

Ukukhetha usayizi ofanele we-vial kubalulekile ukunciphisa ukulahleka kwesampula. Njengomthetho ojwayelekile, ivolumu yesampula kufanele ifane nesilinganiselo se-vial. Usebenzisa i-vial enkulu kakhulu ngesampula ingadala i-headpace eningi kakhulu. Lokhu kwandisa ingozi yokuhwamuka, ikakhulukazi ukuhlaziya okuguquguqukayo. Ngakolunye uhlangothi, ukukhetha i-vial emincane kakhulu kungahle kuholele ekuchithekeni kwesampula nokulahleka.

1.Assess ivolumu yesampula:

Kala noma ulinganise ivolumu yesampula yakho ye-HPLC.

Cabanga ushintsho olulindelekile lwevolumu. Lezi zinguquko zingahle zibe khona ngenxa yokujabula, ukubuyiselwa kabusha, noma ezinye izinyathelo zokulungiselela.

2.Kwaba yivolumu eyisampula kusayizi ojwayelekile we-vial:

Ngemiqulu emincane yesampula (150-300 μl), ukufakwa okuncane kubekwe ngaphakathi kwe-vial enkulu (e.g., 2 ML) kuyanconywa.

Okwe-Medium Samples Volumes (0.5-2 ml), ama-vials ajwayelekile ayi-1.5-2 ml ngokuvamile akhetheke kakhulu.

Ngemiqulu ebabazekayo yesampula (kuze kufike ku-3 ml), ama-4 ml vials angahle afaneleke kakhulu.

3.Consed the headpace:

Ihlose ukunciphisa i-headpace (ivolumu engenalutho) ngaphakathi kwe-vial.

I-headspace edlula ngokweqile ingakhulisa ubungozi bokuhwamuka kwesampula, ikakhulukazi ukuhlaziya okuguquguqukayo.

IVabuli yesampula kufanele lixhumane ne-septum ye-vial noma i-cap ukudala uphawu olunzima.

I-4.Evalisaalisange isidingo sokufakwa:

Ngemiqulu emincane yesampula, ukufakwa okuncane ku-vial enkulu kunganciphisa i-headpace. Lokhu kusetha futhi kunciphisa ukubonakaliswa kwesampula.

Ukufakwa okuncane kuza kumavolumu ahlukahlukene, njenge-150- 300 μL. Bangakwazi ukulingana nosayizi wesampula ohlukile.

Ufuna ukwazi ukuthi ungakhetha kanjani uhlobo olufanele lokufaka okuncane kwesivinini se-Chromatography Vaials Ungayikhetha kanjani uhlobo olufanele lokufakwa kwe-Micro-Insert for Chromatogragraphy Vials yakho

5.Test futhi uqinisekise:

Yenza izivivinyo zokuqala usebenzisa usayizi we-vial okhethiwe nanoma yikuphi ukufakwa.

Gada ukululama kwesampula, ukuqina, kanye nanoma yiziphi izimpawu zokuhwamuka noma zokuwohloka.

Lungisa usayizi we-vial. Noma, sebenzisa ukufakwa njengoba kudingeka. Lokhu kuzokwenza kahle ukuphathwa kwesampula nokulondolozwa.

Landela lezi zinyathelo. Ungathola usayizi omuhle kakhulu we-HPLC we-vial wevolumu yakho yesampula. Kuzosiza futhi ukunciphisa ukulahleka kwesampula. Khumbula ukubheka yonke indawo yokuhamba ngesampula yokusebenza. Futhi, cabanga noma yiziphi izidingo ezikhethekile zokuhlaziya lapho ukhetha.

Ufuna ukwazi ukuthi ungayikhetha kanjani i-cap efanelekile ye-chromatography vial yakho, sicela uhlole le ndatshana Ungayikhetha kanjani uhlobo olufanele lokufakwa kwezinsimbi zakho ze-Chromatography

Ukunciphisa i-headpace nokuvezwa

Into ebaluleke kakhulu ukukwazi ukukhetha ivolumu ye-vial evumelana kangcono nenani lamasampula. Lokhu kusiza ukunciphisa i-headpace futhi kuncishiswe indawo engaphezulu evezwe endaweni ezungezile. Ngokuqhathanisa ngokucophelela usayizi we-vial ku-tampula yethamu, unganciphisa ngempumelelo ukulahleka kwesampula ngenxa yokuhwamuka noma ukuchitheka.

Uma inani lesampula lincane futhi kusetshenziswa i-vial enkulu, i-headpace ngokweqile noma ivolumu engenalutho izokwakhiwa kwi-vial, ezosheshisa ukuhwamuka kwesampula futhi iyekethise ubuqotho besampula. Ukunciphisa ubungozi, usayizi omncane kakhulu we-vial ongahlalisanisa ivolumu yesampula ngenkathi usavumela ukubekwa uphawu okufanele futhi kusetshenziswe. Ukuqinisekisa ukuthi isampula lixhumana ngqo ne-vial septum noma i-cap isiza ukudala uphawu olunzima futhi lunciphise indawo eveziwe engaphezulu.

Ukufakwa kwe-vial kwe-vial

Ukufakwa kwe-vial, ikakhulukazi ukufakwa okuncane, kunganikeza ungqimba owengeziwe wokuvikelwa kwamanani amancane wesampula. Lokhu kufakwa kulunge ngaphakathi kosayizi abakhulu be-vial, ukunciphisa ngempumelelo i-headpace etholakalayo kanye nokunciphisa ukuvezwa kwesampula. Isibonelo, ungaqinisekisa ukuthembeka kwamavolumu amancane wesampula ngokufaka okufakiwe ku-1.5-2 ml vials.

Ukugcina

Ukukhetha ivolumu ye-vial ye-vial efanelekile ye-HPLC autosampler ibaluleke kakhulu ukugcina ubuqotho bokuhlaziywa kwesampula yakho. Kulesi sihloko, sifunde ngamavolumu ajwayelekile we-vial, ukuthi ungasikhetha kanjani i-vial efanele, nokuthi kungani ebalulekile. Sizokhetha i-vial efanele ukunciphisa ukulahleka kwesampula futhi sinikeze idatha ethembekile.

Hlanza kuzicelo ezahlukahlukene ze-Chromatogragraphy Vavials ekuhlolweni kokudla nokuvivinya isiphuzo, ukuhlola iqhaza labo elibalulekile ekuqinisekiseni amazinga okuphepha kanye nekhwalithi:I-HPLC isampula izixazululo zokulungiselela imiphumela emihle kakhulu

Okwedlule:

Wake wayazi yini uhla lwamanani we-HPLC Vavials?

Olandelayo:

Ukubaluleka kwama-HPLC Vavials aqinisekisiwe wokuhlaziywa kwesampula okunembile