Ukuncipha kwesampula kufaka yonke imisebenzi ngaphambi komjovo. Ngaphandle kokuncipha, ukuncibilika, kanye ne-dilution, kuhilela nezinyathelo ezilandelayo:

Ukuzokulungwa
Isizinda
Ukutholwa (Ukutholwa Kwangaphambi Kwekholomu)
I-Chromatography e-Liquid (i-chromatography ephansi ye-chromatography)

Ukwenziwa sengathi kungenziwa ngesandla noma okuzenzakalelayo. Ngabe ujwayelene nokwenziwa kwesampula?

Ukusulwa

Amanye amasampula asakwazi ukufakwa ngokuqondile ngemuva kokuhlekisa. Ukutholwa kudingekile ukwenza amakhompiyutha ngaphandle kokutholwa kwe-UV noma i-fluorescence etholakala ngokutholwa kwe-UV noma nge-fluorescence.

Kuthuthukisa ukuzwela
Kuthuthukisa ukwahlukanisa (ushintsho lwekhwalithi)

Izinkinga ezingenzeka zifaka ukulahleka kwesampula, ukungcoliswa, ukuphendula okungaphelele, noma imikhiqizo eminingi, engathinta ukunemba noma ukwethula amaphutha.

Isampula Filtration

Amasampula we-LC kumele afane futhi ayinamkhawulo; Izinhlayiya zingalimaza isijobelelo noma zivimbe ikholomu. Izinyathelo zifaka:

Nyakazisa isampula ngaphambi komjovo futhi uhlole izinhlayiya, ukuguquguquka, noma ama-emulsions
Uma izinhlayiya zibonwa, hlunga isampula usebenzisa ulwelwesi olungagcina izinhlayiya ze-≥0.15μM

Izinkinga ezingaba khona: Ukungcoliswa kwesampula, ukunciphisa ukuncishiswa kwezakhi kwezakhi, kanye nokuphuma kwe-solvent ukuhwamuka okubangela amaphutha.

Izindlela Zokukhishwa

I-Extraction ihlukanisa ukuhlaziya kusuka kuma-So-solvents noma isusa izinto eziphazamisayo (e.g., amaprotheni). I-Organic Solvents kufanele ibe nobuthi obuphansi, iguquguqukayo, iphansi ngokungcola, bese ichithwa kahle isampula.

Izixazululo ezijwayelekile: UDiethyl Ether, ethyl acetate, Dichloromethane, Chloroform, Benzene, noma Ukuhlanganiswa.

Ngemuva kokukhishwa, amasampula angafakwa ngokuqondile noma agxile ekunyuseni ukugxilisa ingqondo nokuzwela.

Isizinda sesampula esingenamanzi

Izakhi ze-Acidic kanye nosawoti: Ukukhipha ukungcola nge-organic solvent, shintshanisa ne-acidic, bese ukhipha noma ujova; noma womile ngaphansi kwe-N₂ futhi uncibilike kwi-solvent efanele ngaphambi komjovo.
Izakhi eziyisisekelo nosawoti: Ukukhipha ukungcola nge-organic solvent, lungisa okuyisisekelo, bese ukhipha noma ujova; noma womile ngaphansi kwe-N₂ futhi uncibilike kwi-solvent efanele ngaphambi komjovo.
Izakhi ezingathathi hlangothi: Ukukhipha ukungcola nge-organic solvent, bese ngihlaziye ngokuqondile nge-chromatography ye-chromatogragraphy ehleliwe.

I-Fat-soluble Sales Extraction

Khipha nge-solvent ye-organic noma ijove ngqo; noma womile ngaphansi kwe-N₂ futhi uncibilike kwi-solvent efanele ngaphambi komjovo.

Imithombo yokungcolisa

Imithombo engaba khona yokungcoliswa kokuhlaziywa:

Uhlobo Lokungcola	Umthombo	Izindlela Zokuvimbela
-Yimzukeni	Amagesi ayingozi, uthuli, ama-aerosols	Gcina igumbi lezinsimbi eledwa, lihlanzekile; Faka i-AC; lawula umswakama <70%; Vikela umswakama, ukugqwala, ukudlidliza
Izitsha	Ingilazi, Ceramic, Quartz, Labware Plastic	Khetha iziqukathi ezifanele uhlobo lwesampula ngayinye; Hlanza kahle
Reagents	Ama-Recegents we-non-chromatography-grade	Sebenzisa i-chromatography-grade, ubumsulwa obuphezulu, noma ama-reagents webanga lokuhlaziya

Ukulungiselela Isixazululo Esijwayelekile

Lungiselela isisombululo esijwayelekile sangaphakathi kuqala; Sebenzisa i-batch efanayo kumazinga namasampula ukunciphisa amaphutha
Izinto kumele zibe yi-chromatography-grade futhi zizinze
Gcina izixazululo ezijwayelekile emabhodleleni ansundu ngokushisa okuphansi
Ngamavithamini, sebenzisa amaflashi e-brown Volumetric noma avikele ekukhanyeni

Izinkinga ezijwayelekile ekunciphisweni kwesampula kanye nezixazululo

Inkinga	Dala	Isixazululo
Iziqongo ezihlobene ne-Chromatogram	Ukungcola Kokuhlunga	Cwilisa isihlungi sesampula solvent kanye nomjovo wokuhlola Shintsha uhlobo lokuhlunga Sebenzisa amanye amasu wokuhlanza
Ukuphendula okuphezulu okuphansi (ikakhulukazi amasampula aphansi aphansi)	I-adsorption on Fingermur	Shintsha uhlobo lokuhlunga Ukucubungula wonke amasampula ngaphansi kwezimo ezifanayo Sebenzisa amanye amasu wokuhlanza
Ukululama okuphansi	Ukukhishwa okungaphelele	Khulisa isikhathi sokukhishwa, sebenzisa i-solvent eshisayo Lungisa indlela yokuhlanza
Iziqongo ezibanzi, isikhathi sokufiphala ikholamu	Ukuphazamiseka kwesampula nokungcola	Thuthukisa indlela yokuhlanza
Ukunemba okungekuhle	Ukuvuselelwa okungaphelele	Lungiselela noma ubeke esikhundleni sokutholwa, ukwahlukana, ukukhishwa, noma ezinye izimo Sebenzisa ukuphatha okuzenzakalelayo ukuze uthuthukise ukucaciswa

Okwedlule:

Ukushisa okuphezulu kakhulu kanye nemiphumela yokuvezwa kokukhanya okuphansi kokuqina kwesampula: Ithiyori & Methol

Olandelayo:

Buyela ohlwini