I-HPLC ihluke kanjani kwikholomu ye-GC?

I-HPLC (i-chromatography ephezulu yokusebenza kwe-chromatography) ne-GC (i-chromatography yegesi) zombili izindlela zokuhlaziya ezinamandla ezisetshenziselwa ukuhlukanisa, ukukhomba, kanye nezinhlanganisela ezahlukahlukene kumasampula ahlukahlukene. Kodwa-ke, zihluke kakhulu ngokuya ngokusebenza, imishini, kanye nezicelo. Lo mbhalo uveza umehluko obalulekile phakathi kwamakholomu we-HPLC ne-GC, ugxile ekuklameni kwabo, ukusebenza, kanye nokufaneleka kwezinhlobo ezahlukene zokuhlaziywa.

Idizayini yekholomu

Amakholomu we-HPLC

Amakholomu we-HPLC amfishane futhi abanzi kunamakholamu we-GC. Ngokuvamile zifinyelela kuma-30 cm ubude futhi zibe nobubanzi bangaphakathi ezisukela ku-2.1 mm kuya ku-8 mm. Ukupakishwa ngaphakathi kwamakholomu we-HPLC kuqukethe izinhlayiya ezincane (imvamisa ezingaphansi kwama-microns ezingama-5 ububanzi) ezinikezela indawo enkulu engaphezulu yokuxhumana nezakhi zesampula. Izakhiwo zokupakisha zalezi zinkundla zibavumela ukuba bahlukanise kahle amakhompiyutha asuselwa kwizakhiwo zawo zamakhemikhali.

Izici eziyinhloko:

Ubude: Kufika ku-30 ​​cm

Ububanzi: Ngokuvamile phakathi kuka-2.1 mm no-8 mm

Izinto zokupakisha: izinhlayiya ezincane (e.g. Silica) ngokuguqulwa okuhlukahlukene komhlaba ofanele izindlela ezahlukahlukene zokuhlukanisa (isb. Isigaba esibuyiselwe emuva, isigaba esijwayelekile).

Amakholomu we-GC

Amakholamu we-GC, ngokuqhathanisa, ade futhi anciphise, ajwayele ukuya kumamitha ayi-100 futhi abe nobubanzi bangaphakathi kusuka ku-0,1 mm kuya ku-1 mm. Zingahlukaniswa ngezinhlobo ezimbili eziphambili: amakholomu agcwele amakholomu namakholomu we-capillary. Amakholomu agcwele aqukethe isigaba esiqinile noma uketshezi oluhlanganiswe ngokusekelwa okuqinile, kanti amakholomu e-capillary anefilimu emincane yesigaba esimisiwe sodonga lwangaphakathi.

Izici eziyinhloko:

Ubude: Kufika ku-100 m

Ububanzi: Ngokuvamile phakathi kuka-0.1 mm no-1 mm

Izinhlobo zekholomu ezigcwele (ezihlanganisiwe (eziqinile noma zesiteshi sezinto) kanye namakholomu we-capillary (avulekele isakhiwo esivulekile se-tubular).

Isigaba

I-Chromatography ephezulu yokusebenza kwe-chromatography

Ku-HPLC, isigaba seselula ngokuvamile i-solvent ewuketshezi noma ingxube ye-polar noma i-solvents engeyona eye-polar. Izixazululo ezijwayelekile zifaka amanzi, i-methanol, i-acetonitrile, kanye nama-buffers ahlukahlukene. Ukukhethwa kwesigaba seselula kubalulekile ngoba kuthinta ukuxhumana phakathi kwe-analyte kanye nesigaba seminyaka ngaphakathi kwekholomu.

Igesi i-chromatography

I-GC isebenzisa isigaba se-Gaseous Mobile Mobile, imvamisa kakhulu igesi e-inert efana ne-helium noma i-nitrogen. Isampula kufanele ibe yinqwaba yokwanela uma ingeniswa kukholamu. Le mfuneko isho ukuthi i-GC ilungele kakhulu ukuhlaziya amakhompiyutha aguqukayo, kuyilapho i-HPLC ingaphatha izinto ezibanzi ezibanzi, kufaka phakathi amakhompiyutha angewona awodwa.

Indlela yokuhlukanisa

I-Chromatography ephezulu yokusebenza kwe-chromatography

I-HPLC ihlukanisa amakhompiyutha asuselwa kubumbano lwabo lwesigaba seminyaka esihlobene nesigaba seselula. Izinhlobo ezahlukahlukene ze-chromatography zingasetshenziswa:

I-Chromatography yesigaba sangemuva: Isigaba se-Nopolar Statical Station ngesigaba se-Polar Mobile.

I-Chromatography ejwayelekile yesigaba: Isigaba se-Polar Statical esinesigaba se-Nongpolar Mobile.

I-ION Exchange Chromatography: Ihlukanisa izinhlobo ezikhokhiswayo ezisuselwa ekuxhumaneni kwazo nesigaba esibekiwe.

I-Chromatography yesayizi yosayizi: Ihlukanisa ama-molecule ngokuya ngosayizi.

Igesi i-chromatography

Kwi-chromatography yegesi, ukwahlukana kutholakala ngokuyinhloko umehluko ekuguqukeni kwamaphuzu wokuqina kanye namaphuzu abilayo okuhlaziya. Amakhompiyutha avele kalula azovutha asuka kukholamu kuqala, kuyilapho amakhompiyutha amancane ashubile azothatha isikhathi eside ukudlula. Ukusebenzisana phakathi kwe-analyte kanye nesigaba seminyaka kungathinta nesikhathi sokugcina.

Ukuzwela nokuxazululwa

I-HPLC Sensitivity

I-HPLC ngokuvamile inokuzwela okuphezulu kwamakhompiyutha angewona ama-angewonakele ngoba iyakwazi ukuhlaziya ukugxila okuphansi kwamasampula ngaphandle kokuhwamuka. Kusetshenziswa osayizi abancane bezinhlayiyana kumakholomu we-HPLC kuhlinzeka indawo enkulu engaphezulu yokusebenzisana, okuthuthukisa ukulungiswa.

Ukuzwela kwe-GC

Njengoba i-chromatography yegesi ikwazi ukugxila ehlaziyo ngokuhwamuka, iyakwazi ukufeza ukuzwela okuphezulu kwamakhompiyutha aguqukayo. Amakholamu we-capillary ngokuvamile anokuxazululwa okungcono kunamakholomu agcwele ngenxa yobude bawo obude nobubanzi obuncane.

Izicelo ze-HPLC ne-GC

Izicelo ze-HPLC

I-HPLC isetshenziswa ezinhlobonhlobo zamasimu ngenxa yokusebenza kwayo okuhlukahlukene:

Ukuhlaziywa Kwemithi: Kusetshenziselwa Ukuhlolwa Kwezidakamizwa Nokulawulwa Kwekhwalithi.

Ukuhlolwa Kwezemvelo: hlaziya ama-contarts emanzini kanye namasampula emhlabathini.

Ukuhlolwa kokuphepha kokudla: Thola ukungcola futhi uqinisekise ikhwalithi yokudla.

I-Biotechnology: hlanza amaprotheni nama-nucleic acid.

Izicelo zezicelo ze-Chromatography

I-GC isetshenziselwa ikakhulukazi ukuhlaziya amakhompiyutha ajwayelekile aphilayo:

Ukuhlaziywa Kwezemvelo: Ukulinganisa amakhompiyutha aguqukayo eziphilayo ezindaweni zokungcola komoya namanzi.

Isayensi ye-Forensic: Ukuhlaziya izinto zokwakha izigcawu zobugebengu.

Umkhakha we-Petrochemical: Uphawu lweHydrocarbons kumafutha.

Ukuhlaziywa kwe-flavour nephunga: Ukuhlonza izakhi eziguqukayo ekudleni.

Ngamafuphi, i-HPLC ne-GC yizindlela ezahlukahlukene ze-Chromatographic ezilungele izinhlobo ezahlukene zokuhlaziywa ngokusekelwe ekwakhiweni kwamakholomu zazo, isigaba seselula, indlela yokuhlukanisa, ukuzwela, ukuzwela, ukuzwela, ukuzwela, ukuzwela, ukuzwela, ukuzwela, ukuzwela, ukuzwela, ukuzwela, ukuzwela, ukuzwela, ukuzwela, ukuzwela, ukuzwela, ukuzwela, ukuzwela, ukuzwela, ukuzwela, ukuzwela, ukuzwela, ukuzwela, ukuzwela, ukuzwela, ukuzwela, ukuzwela kwamakhono. I-HPLC ilungele amakhompiyutha angewona aguqukayo noma ashisayo adinga isigaba se-liquid mobile, ngenkathi ama-GC excels ekuhlanyeniseleni izinto eziguqukayo usebenzisa isigaba se-Gaseous Mobile. Ukuqonda lo mehluko kuvumela abacwaningi ukuthi bakhethe indlela efanelekile yezidingo zabo ezithile zokuhlaziya, baqinisekise imiphumela efanelekile emasimini esayensi ahlukahlukene.

Ngabe uyawazi umehluko phakathi kwe-HPLC VIALS kanye nama-GC vials? Bheka le ndatshana: Uyini umehluko phakathi kwama-HPLC vials nama-GC vials?