Yiziphi izinhlobo zokuthola kabusha?

Ama-vials aphelele wokutakula, owaziwa nangokuthiAma-vials aphezulu aphezulunoma izipesheli ezincane, iziqukathi zesampula eziklanywe ngokukhethekile zenzelwe ukunciphisa i-haryover futhi zandise ukululama kwesampula. Lokhu bakufeza lokhu ngomklamo wangaphakathi ohlukile, ngokujwayelekile nge-tapered noma enobumba obunjenge-funnel ehlanganisa uketshezi ezansi. Lokhu kuvumela inaliti ye-autosampler ukuthintana cishe nayo yonke lesampula, inciphise imfucuza kanye nokwanda kokuzwela kokuhlaziywa.

Ufuna ukwazi kabanzi ngama-HPLC avuselela ama-HPLC, sicela uhlole le ndatshana:Ama-HPLC avuselela ama-HPLC aphezulu

Izici ezibalulekile nezinzuzo

I-CaryOver encane: Inzuzo eyinhloko ye-vial ephelele yokubuyisa yikhono layo ukunciphisa i-Carronder kuya kumaMicroliters ambalwa (ngokuvamile azungeze i-1μL), kanti ama-vials ajwayelekile ashiya i-Carrover ebalulekile.

Ukuvuselelwa kwesampula okwenziwe ngcono

Ukuzwela okwenziwe ngcono: Ngokunciphisa ukulahleka kwesampula, ama-vials aphelele athola ukuthuthukisa ukuzwela kokuhlaziya, ikakhulukazi uma usebenza ngokuhlaziywa okuhlushwa okuphansi.

Ukuvumelana kwe-Autosampler: Ama-vials aphelele wokululama aklanyelwe ukuhambisana nama-autosamplers ajwayelekile, aqinisekise ukuhlanganiswa komthungo ekuhambeni kwakho komsebenzi okukhona. Kodwa-ke, kungadingeka ukuthi uguqule inalithi yokuphakama yenaliti.

Ilungele amasampula ayigugu: Uma usebenza ngamasampula anqunyelwe noma abizayo, ama-vials aphelele athola ukufundwa ukuze aqinisekise ukuthi uthola idatha eningi ngangokunokwenzeka.

Ucezu olulodwa noma umklamo ohlanganisiwe: izimbotshana zokutakula zivame ukuba yingilazi ezihlanganisiwe nge-liner yengilazi ebekwe kubo ngesikhathi senqubo yokwenza. I-liner esetshenzisiwe imvamisa i-liner ehlakaniphile.

Ukukhetha i-vial ephelele yokuthola kabusha

Ukukhetha i-vial ephelele yokubuyisa kuncike ezicini eziningana:

IVampula yesampula: Cabanga ngevolumu ejwayelekile yesampula yakho. Amanye ama-vials aphelele atholakele alungiselelwe amavolumu amancane kakhulu (e.g., <100 μl), kanti amanye angathola amavolumu amakhulu (aze afike ku-1.5 ml noma ngaphezulu). Iziqukathi ze-10μL zigxile ekhasini elingezansi kwe-vial, eqinisekisa ukululama okuphezulu noma ngabe kusebenza amasampula alandelwayo

Ukuvumelana kwe-Autosampler: Qiniseka ukuthi i-vial iyahambisana nemodeli yakho ye-autosampler. Bheka ubukhulu be-vial (ukuphakama nobubanzi) nohlobo lokuvalwa (isikulufa cap, crimp cap, njll.).

Ukuhambisana Kwezinto Ezibonakalayo: Khetha ama-vials enziwe ngezinto ezihambelana ne-solvent kanye ne-analyte. Ingilazi ye-borosilucate ukukhetha okuvamile ngenxa yokuhlanekezelwa kwamakhemikhali.

Uhlobo lokuvalwa: Khetha ukuvalwa okunamathiselwe kahle ukuvikela ukuhwamuka nokungcola.Ptfe \ abikoneyizinto ezivamile ze-septum.

Ukucatshangelwa okusebenzayo namathiphu

Ukulungiswa kwe-NIKELLE ukuphakama: Uma usebenzisa i-Vality Suiled Valials, ukulungiswa kwenaliti ku-autosampler kumele kulungiswe ukuze inaliti ifinyelela ezansi kwesitsha se-conical.

Isitifiketi se-Vial: Ngezicelo ezibucayi, cabanga ngokusebenzisa ama-vials aqinisekisiwe ahlolwe ukuhlanzeka nokuphazamiseka kwangemuva okuphansi.

Isitoreji: Gcina ama-vials endaweni ehlanzekile, eyomile ukuvikela ukungcoliswa.

Izindleko: Ama-vials aphezulu wokutakula autosampler angabiza kakhulu kunama-vials ajwayelekile

Ingqikithi yokuthola kabushaiyithuluzi elisemqoka lamakhemikhali wokuhlaziya asebenza namasampula akhawulelwe noma ayigugu. Idizayini yabo eyingqayizivele nekhono lokunciphisa i-CarthOver ibenze balithumele ngethuluzi lokukhulisa ukululama kwesampula, ukuzwela okwandayo, kanye nokuqinisekisa imiphumela efanele. Ngokucabanga ngokucophelela izidingo zakho ezithile bese ukhetha uhlobo olufanele lwe-vial ephelele yokuthola kabusha, ungabona amandla akho agcwele futhi uthola ukuqonda okubalulekile kumasampula akho amancane.