Igesi Chromatography (GC)kuyindlela ebalulekile yokuhlaziya esetshenziselwa ukuhlukanisa nokuhlaziya amakhompiyutha kwingxube. Enye yezilinganiso ezibalulekile ku-GC indawo ephakeme, ehlobene nokuhlushwa kokuhlaziywa kwesampula. Ukuqonda izinto ezithinta indawo ephezulu kubalulekile ekutholeni imiphumela ethembekile nezenzekayo. Le ndatshana ithatha ukujula okujulile ezintweni ezahlukahlukene ezithinta indawo ye-GC Peak, kufaka phakathi inqubo yokujoveka, izimo zekholomu, izilungiselelo zomtshina, kanye nezimpawu zesampula.

IngomaneImininingwane engaphezulu nge-autosampler vials ye-chromatography yegesi, bhekani le ndatshana: 2 ml autosampler vials we-chromatography yegesi

1. Icebo lokujova

Indlela yokujova kwesampula idlala indima ebalulekile ekunqumeni indawo ephezulu:

Umvivo we-Umjovo: Inani lokujova amasampula kumele libe ngqo. Ukulayisha ngokweqile ikholomu kungadala ukuphambukiswa okuphezulu njengokuphambili noma ukuqondisa, okungathinta indawo ephakathi. Ngakolunye uhlangothi, ivolumu yomjovo enganele ingaholela ekujuleni kwesignali esiphansi kanye nendawo ephakeme ephakeme.

Ijubane lokulimaza: Ijubane lapho isampula lijova futhi lungathinta shamo ephakeme nendawo ephezulu. Imijovo esheshayo ingadala isiyaluyalu, okuholela ekuhlanganiseni okungekuhle nokuhlanganiswa kwe-band, ekugcineni kunciphisa indawo ephezulu.

Imodi yokujova: imijovo ehlukanisayo ivame ukusetshenziselwa ukulandelwa kokulandela umkhondo ngoba ivumela ukuhlaziya okuningi ukuthi kufake ikholomu, indawo ephakeme kakhulu ekhuphukayo. Ngokuphambene nalokho, imijovo ehlukanisayo ifaka isampula, okuholela ezindaweni ezincane eziphakeme ngamasampula agxile kakhulu.

2. Izimo zekholomu

Izici zekholomu zinomthelela omkhulu endaweni ephezulu:

Uhlobo lwekholomu nosayizi: Ukukhethwa kwekholomu (e.g., i-capillary vs. iphaketheni) iyathinta ukulungiswa nokusebenza kahle. Amakholamu we-capillary ngokuvamile anobubanzi obuncane kanye nendawo enkulu yendawo engaphezulu, okuholela ekuhlukaniseni okungcono kanye neziqongo ezibukhali.

Ukushisa kwekholomu: Ukulawulwa kokushisa kubalulekile ku-GC. Amazinga okushisa aphezulu angathuthukisa ukuguquguquka futhi athuthukise ukuqedwa, kepha angaholela eziqongweni ezibanzi uma zingalawulwa kahle. Ngakolunye uhlangothi, amazinga okushisa aphansi angaholela ezikhathini zokugcina isikhathi eside kanye nezindawo eziphakeme kakhulu ngenxa yokwahlukaniswa okuthuthukile.

Izinga lokugeleza kwegesi yenethiwekhi: izinga lokugeleza kwegesi yenethiwekhi lithinta ukuthi amahlaya ahamba ngokushesha kangakanani kukholamu. Isilinganiso esifanele sokugeleza siqinisekisa iziqongo ezibukhali; Kuphakama kakhulu kube yimiphumela yokugeleza kwesilinganiso ekunciphiseni phakathi kwe-analyte kanye nesigaba seminyaka, okuholela ezindaweni ezincane kakhulu.

Ngabe uyawazi umehluko phakathi kwe-HPLC VIALS kanye nama-GC vials? Bheka le ndatshana:Uyini umehluko phakathi kwama-HPLC vials nama-GC vials?

3. Izilungiselelo zomhloli

Ukusebenza komtshina kuthinta ngqo ukunemba kwesilinganiso sendawo ephezulu:

Ukuzwela kwe-Detector: Ukuthola okuhlukahlukene kunengqondo ehlukile yokuhlaziya. Isibonelo, umtshina we-Flame Ionization (Fid) uzwela kakhulu, kepha kungenzeka angaphenduli ngokulinganayo kuzo zonke izinhlaka, ezithinta indawo ephakeme ephakeme.

Isikhathi Sokuphendula: Isikhathi sokuphendula somtshina sithinta ukuthi sishesha kangakanani ukubhekana nezinguquko ekugxileni kokuhlaziywa. Izikhathi zokuphendula ezihamba kancane ziphumela eziqongweni ezibanzi, ezinciphisa indawo ebaliwe.

Ukuzilolonga lokushisa: Ukutholwa okuningi kuzwela ekuguquguqukeni kwezinga lokushisa. Ukuqinisekisa ukuthi izinga lokushisa elizinzile lisiza ukugcina impendulo engaguquki kanye nesilinganiso esinembile sendawo ephezulu.

4. Izici zesampula

Uhlobo lwesampula elihlaziywa luyabanda iqhaza elibalulekile:

Ukugxila: Ukuhlushwa kokuhlaziya kuthinta ngqo indawo ephezulu; okuphezulu okuhlushwa, kukhudlwana indawo ephezulu. Kodwa-ke, uma ukugxila kudlula uhla lwe-linear lomtshina, ukugcwala kanye nezilinganiso ezinganembile kungahle kube nomphumela.

Imiphumela ye-Matrix: Ukuba khona kwamanye amakhompiyutha ku-matrix eyinkimbinkimbi kungaphazamisa ukutholwa kokuhlaziya. Izakhi ze-matrix zingadonsa noma zisabele nge-analyte, okuholele endaweni encishisiwe yendawo noma izikhathi zokugcina.

Ukuguquguquka: Ukuguquguquka kokuthile okuhlaziya kuthinta indlela yokuziphatha kwayo ngesikhathi se-chromatography. Amakhompiyutha aguquguqukayo amaningi ashesha futhi angakhiqiza iziqongo ezibukhali kunamakhompiyutha amancane aguqukayo, angahle aholele eziqongweni ezibanzi ngezindawo ezincishisiwe ngoba isikhathi sokugcina seluliwe.

5. Ukusebenza kohlelo

Ukusebenza kohlelo lonke kubalulekile ukuthola imiphumela enembile ye-GC:

Umsindo wesisekelo

Ukusebenza kahle kwekholomu: Kuncishiswe ukusebenza kahle kwekholomu ngenxa yokuguga noma ukungcoliswa kungaholela ekutheni iziqongo ezibanzi nezindawo ezincane. Ukulungiswa njalo nokuqapha ukusebenza kukholamu kubalulekile ekufezekiseni imiphumela engaguquki.

Amapharamitha wokuhlanganisa: isoftware esetshenziselwa ukuhlanganisa iziqongo kufanele ilungiselelwe kahle. Izilungiselelo ezingalungile zingaholela ekubalweni kwendawo eyi-Peak Peak ngenxa yokubekwa kwesisekelo okungalungile noma imibundu yokuhlanganiswa.

Ufuna ukwazi kabanzi ngomehluko phakathi kwe-LC-MS ne-GC-MS, sicela uhlole le ndatshana: Uyini umehluko phakathi kwe-LC-MS ne-GC-MS?

Ukugcina

Izindawo eziphakeme ze-GC zithintekile yizici ezahlukahlukene, kufaka phakathi inqubo yokujoveka nezimo zekholomu, izilungiselelo zomshini, nezimpawu zesampula. Ukuqinisekisa imininingwane esezingeni eliphakeme, abaphenyi balawula ngokucophelela ivolumu yomjovo kanye nesivinini, bandisa izimo zekholomu ezifana nezinga lokushisa kanye nokugeleza kwesilinganiso sokugeleza kanye nokusebenza kokusebenza kwe-detector ngokulinganisa okujwayelekile. Kudingeka futhi bacabangele imiphumela yesampula ye-matrix lapho elungiselela amasampula. Ngokubhekana nalezi zinkinga, ama-laboratories angathuthukisa ukunemba nokwethenjwa kokuhlaziywa kwe-chromatographic, ekugcineni ukuthola imiphumela yokuhlaziya ethembekile.

Okwedlule:

Yini i-toc vial nokubaluleka kwayo ku-chromatography?

Olandelayo:

I-Cod yokugaya ama-vials (HG-free): okudingeka ukwazi