Uma kukhulunywa ngokuqinisekisa ubuqotho kanye nokwethenjwa kwemiphumela yokuhlaziya kwizilungiselelo zelebhu, ukukhetha kweushikukaziI-yring finger impahlakubalulekile. Izinto ezahlukene zibonisa ukusebenza okuhlukahlukene kokugcinwa kabusha kokugcinwa kwe-particle, okungathinta kakhulu ikhwalithi yamasampula elungiselelwe ukuhlaziywa, ikakhulukazi kumasu afana ne-chromatography ephezulu ye-chromatomatography (HPLC).

Imibuzo ebuzwa njalo mayelana nokuhlunga ngezimpawu, qiniseka ukuthi ubheka le ndatshana efundisayo:Isihloko se- "Syringe Filter" Imibuzo Ebuzwa Njalo Kaningi

Izici ezisemqoka ukuthonya ukusebenza kahle kwezinhlayiya

1 usayizi we-pore

Usayizi we-pore of a syringe ungenye yezinto ezibaluleke kakhulu ezithinta ukusebenza kahle kwayo kokugcina izinhlayiya. Amasayizi ajwayelekile we-pore ahlanganisa:

0.22 μm ushikukazichring Isihlungi: Lezi zihlungi zilungele izinhlelo zokusebenza ezidinga ukususwa kwamagciwane nezinhlayiya ezincane. Zivame ukusetshenziswa ezinqubweni zokubulala inzalo kanye nokuhlunga imidiya yesiko leselula. Ngenxa yosayizi wabo omuhle we-pore, bahlinzeka ngezinga eliphakeme lokugcinwa kwe-particle, evame ukwedlula i-98% yezinhlayiya ezinkulu kune-0.22 μm.

0.45 μm ushikukazichring Isihlungi: Lezi zihlungi zilungele ukususwa okujwayelekile kwezinhlayiyana, ukuzenza isinqumo esithandwayo sokulungiselela isampuli ye-HPLC. Ngenkathi zivumela amanani wokugeleza okusheshayo aqhathaniswa nokuhlunga okungu-0.22 μm, angagcina izinhlayiya ezimbalwa, ngokuvamile cishe ngo-90-95% wezinhlayiya ezinkulu kune-0.45 μm.

Ukukhetha usayizi ofanele we-pore kudinga ukulinganiswa kokuhlungwa kwe-fieltration kanye nokugeleza kwesilinganiso. Amasayizi amancane e-pore anikela ngokugcinwa okuphezulu kepha anciphise inqubo yokuhlunga.

Ufuna ukwazi ulwazi oluphelele mayelana nesihlungi se-syrinji, sicela uhlole le ndatshana:Umhlahlandlela ophelele wokuhlunga kwesirinji: Izici, Ukukhetha, Intengo, kanye nokusebenzisa

2. Ukuhlunga okubonakalayo

Izinto zokuhlunga zesirinji nazo zidlala indima ebalulekile ekunqumeni ukusebenza kahle kwayo kokugcina. Nazi ezinye izinto ezivamile ezisetshenziswa ekuhlunga kwesirinji:

I-Polytetrafluoroethylene (PTFE): Yaziwa ngokumelana kwayo okuhle kwamakhemikhali,I-PTFE ushikukazichring isihlungizilungele izixazululo ezinolaka kanye nezicelo eziphakeme zokushisa. Ngokuvamile bakhombisa amanani aphezulu okugcina izinhlayiya, imvamisa cishe ngo-98-100% wezinhlayiki ezinkulu kune-0.45 μm, okwenza babe nelungele izinhlelo ze-HPLC.

Nylon: Injobe ushikukazichring isihlungiziguquguqukayo futhi zingasetjenziselwa solvents enamanzi kanye ne-organic. Bakhombisa ukugcinwa okuhle kwezinhlayiyana kodwa bangaba nokusebenza okuphansi okuqhathaniswa nokuhlunga kwe-PTFE, ikakhulukazi ezindaweni ezinolaka kwamakhemikhali.

Polyethersulfone (PES): Izimpondana ushikukazichring isihlungiZaziwa ngezakhiwo zazo eziphansi ezibophayo zamaprotheni, zibenza balungele ukuhlunga kwesampula yesayensi. Bahlinzeka ngamanani amahle okugcina izinhlayiyana, imvamisa ezungeze ama-95-98% ezihlungi ze-0.22 μm.

I-Revenerated Cellulose (RC): Izihlungi zesirinji ze-RC zivame ukusetshenziselwa izixazululo zamanzi futhi zinikeze ukusebenza kahle kwe-parterye returence. Kodwa-ke, izifundo zikhombisile ukuthi izihlungi ze-RC zingaba namazinga aphansi okugcina, kwesinye isikhathi kuphansi njenge-48% izihlungi ezingama-0.45 μm, okusho ukuthi ingxenye ebalulekile yezinhlayiya ingadlula.

Ama-cellulose esters ahlanganisiwe (MCE):Uhlobo lwensimbi ushikukazichring isihlungi zinamandla futhi zilungele izixazululo zamanzi. Zivame ukusetshenziswa ekuhlaziyweni kwe-microbiological ngenxa yokugcinwa kwazo okuhle kwama-microorganisms, kepha ukusebenza kwawo kungahluka ngokuya ngesicelo esithile.

3. Ukuhlunga ububanzi nokuklama

Ububanzi bokuhlunga kwesirinji kungathonya nokusebenza kwaso kokugcina. Ukuhlunga okukhulu kwe-diameter kungaba nokuphamba okuphezulu, okuvumela amanani okuhlunga asheshayo, kepha angahle abe nokusatshalaliswa okuhlukile kosayizi we-pore, okuthinta ukusebenza kahle kokugcina. Ngokwengeziwe, ukwakheka kwesihlungi, njengokuba khona kokuhlunga kwangaphambili noma ukwelashwa okuthile okungaphezulu, kungathuthukisa ukugcinwa kwezinhlayiya.

Ukuqhathanisa ukusebenza kahle kokugcina izinhlayiya kwezihlungi ezihlukile zesirinji

Ngokusekelwe ezintweni okuxoxwe ngazo, nansi isifinyezo sokusebenza kahle kokugcina kwezinhlayiya kwezinto zokuhlunga ezihlukile:

Okokuhlunga okubonakalayo	Usayizi we-pore	Ukusebenza kahle kokugcina	Izicelo ezinhle kakhulu
I-PTFE	0.45 μm	98-100%	Izixazululo ezinolaka, i-HPLC
Injobe	0.45 μm	90-95%	Ukusetshenziswa okujwayelekile, ama-aquod ne-organic
Izimpondana	0.22 μm	95-98%	Amasampula endalo, amaprotheni aphansi abophayo
Ukuvuselela iselula	0.45 μm	48-50%	Izisombululo ezinamanzi, ukuhlaziywa kwe-microbiological
Ama-cellulose esters ahlanganisiwe	0.45 μm	Iyahluka	Ukuhlaziywa kwe-Microbiological

Ukugcina

Ukukhetha i-syringe yokuhlunga okulungile kubalulekile ekuqinisekiseni ukusebenza kahle kwezinhlayiyana ezisetshenziselwa ilabhorethri, ikakhulukazi ukuhlaziywa kwe-HPLC. Ukuhlunga kwe-PTFE ngokuvamile kunikeza ukugcinwa okuphezulu kakhulu, kubenze balungele izixazululo ezinolaka kanye nezicelo ezibucayi. Ngokuphambene, izihlungi ze-cellulose ezivuselelekile zingahlinzeki ukugcinwa okwanele kwezicelo ezithile, ubuqotho bemininingwane okungenzeka.

Lapho ukhetha isihlungi sesirinji, cabanga ngezidingo ezithile zokuhlaziywa kwakho, kufaka phakathi uhlobo lwesampula, ukuhambisana kwe-solvent, kanye nokusebenza kahle kokugcina kabusha. Ngokuqonda umehluko ekugcinweni kwezinhlathihla phakathi kwezinto zokuhlunga ezihlukile, ungenza isinqumo esinolwazi esithuthukisa ikhwalithi kanye nokwethenjwa kwemiphumela yakho yokuhlaziya. Ukuhlunga okufanele akuvikeleli nje ukusetshenziswa kwakho, kodwa futhi kunomthelela empumelelweni ephelele yokusebenza kwelebhu yakho.

Uyazi ukuthi ingabe isihlungi esirinji esingasetshenziswa yini futhi? Sicela ubheke le ndatshana: Okokuhlunga ngezimpawu, uzophinda usebenzise?

Okwedlule:

Pre-slit vial cap vs. solid cap: okungcono ngelebhu yakho?

Olandelayo:

I-HPLC vs Electrophoresis: okufanele uyisebenzise?