Yini ukubaluleka kwezinhlamvu ze-1.5ml GC ekuhlaziyweni kwesampula?

Ukusetshenziswa kwe-vials engu-1.5 mL kaphethiloli ye-chromatography (GC) kubalulekile emkhakheni wamakhemikhali wokuhlaziya, ikakhulukazi ukuhlaziywa kwezinto eziguqukayo ze-organic (ama-VC). Lezi zinhlaka zihlanganiswa ezinhlobonhlobo zezicelo, kufaka phakathi ukuqapha kwemvelo, ukuhlolwa kokuphepha kokudla, nokuhlaziywa kwemithi. Le bhulogi izothambisa ngokujulile ekubalulekeni1.5 ml gc vialsEkuhlaziyweni kwesampula, ukugxila ezinhlelweni zabo, izinhlelo zokusebenza, kanye nemikhuba emihle kakhulu.

Ngeminye imininingwane ngama-autosampler vials we-chromatography yegesi, bheka le ndatshana: 2 ml autosampler vials we-chromatography yegesi

Ukubaluleka kwama-1.5 ml gc vials

1. Ivolumu efanelekile yokuhlaziywa kwe-headSpace

Enye yezizathu eziphambili zokusebenzisa ama-vials angu-1.5 mL Gc vc ukuthi anobukhulu obuhle kakhulu besampula ye-headSpace. Ekuhlaziyweni kwe-headpace, indawo ebekwe uphawu ivumela ama-vocs ukufinyelela ukulingana phakathi kwesampula noma isampula eliqinile kanye nesigaba segesi ngaphezulu kwayo. Ivolumu engu-1.5 ml ishaya ibhalansi phakathi kokuhlinzeka ngekhanda elanele lesampula yegesi elisebenzayo futhi inciphisa amandla okulahleka kwesampula ngenxa ye-adposi.

I-Equilibrium Kinetics: Ivolumu elincane, ukulingana okusheshayo phakathi kwezigaba kungasungulwa, okubaluleke kakhulu ekulinganiseni ukugxila kokuhlaziywa kokuhlaziywa kokuhlaziywa. Ukuhlushwa kwegesi kulokho okwenziwa yi-GC ekugcineni kuhlaziya, ngakho-ke kubalulekile ukuthi lesi sigaba simele isampula.

2. Yehlise ama-adsorption

I-adsorption ingadala ukuthi amakhompiyutha aguqukayo alahlekelwe ezindongeni ze-vial, ezingakuthinta kakhulu ukunemba kwemiphumela yokuhlaziya. Izimo ezisezingeni eliphakeme ezi-1.5 ml ezenziwe ngengilazi eyinhloko ye-hydrolyzed zenzelwe ukunciphisa le miphumela.

Izakhiwo eziphansi ze-adsorption: Kuqinisekisiwe okuningi1.5 ml vialsbaphathwa ukunciphisa i-adsorption, ikakhulukazi amakhompiyutha ayisisekelo. Lokhu kwelashwa kuthuthukisa ukunemba okulinganisayo futhi kuqinisekisa ukuthi ukulandelwa kokulandela umkhondo kuhlala kungahleliwe ngesikhathi sokuhlaziywa.

3. Ukuhambisana nezinhlobonhlobo zamasu wokuhlaziya

Ukuguquguquka okuhlukahlukene kwezingu-1.5 ml vials akunqunyelwe ku-GC; Zifanele futhi kugesi ye-chromatography-mass spectromeetry (izinhlelo ze-GC-MS).

I-Versitile: Iklanyelwe ukwamukela amasu ahlukahlukene wokuhlaziya, avumela ama-labs ukuthi ahambise izinqubo ngaphandle kokulungiselela izinhlobo eziningi zamabhodlela we-reagent ngezindlela ezahlukahlukene. Lokhu kuhambisana kwenza lula ukuphathwa kokusungula futhi kunciphise izindleko.

Izicelo ze-1.5 ml gc vials

1. Ukuqapha imvelo

Ukuthola ukulandelela ukulandelela emoyeni noma kumasampula wamanzi kubalulekile ezifundweni zemvelo.

Ukuhlaziywa kwe-VOC: 1.5 ml vials ilungele ukuthumba ama-vocs kusuka kumasampula ezemvelo ngaphandle kokulahleka okukhulu ngenxa yokuthola ama-adsorption noma ukuhwamuka, ukuqinisekisa ukuthi imiphumela ikhombise ukucaciswa kwesampula.

2. Ukuhlolwa kokuphepha kokudla

Ukuphepha kokudla kubalulekile ekuqinisekiseni impilo yomphakathi, futhi amakhompiyutha aguqukayo akhombisa ukungcola kokudla noma ukungcoliswa.

Ukunambitheka kanye nama-aroma2 ml vialsivumela ukulawulwa kwekhwalithi okunekhwalithi nokuphepha kwezokuphepha.

3. Ukuhlaziywa kwemithi

Ukuhlaziya ukungcola okuguquguqukayo noma izithako ezisebenzayo kubalulekile ekuthuthukisweni kwezidakamizwa kanye nokulawulwa kwekhwalithi.

Ukuhlolwa kokuqina: Amavolumu amancane awusizo ikakhulukazi ezifundweni zokuqina, njengoba kuphela inani elilinganiselwe lezidakamizwa liyatholakala ukuze lihlolwe.

Ngabe uyawazi umehluko phakathi kwe-HPLC VIALS kanye nama-GC vials? Bheka le ndatshana:Uyini umehluko phakathi kwama-HPLC vials nama-GC vials?

Imikhuba emihle kakhulu yokusebenzisa ama-1.5 ml gc vials

1. Amasu afanele wokubopha uphawu

Ukuqinisekisa ukuthi ama-vials avalwe kahle kubalulekile ekuvimbeleni ukuvuza nokungcola:

Sebenzisa i-Septa ephezulu: KhethaupendeKwenziwe ngezinto ezifana ne-PTFE noma i-Silicone, enikezela ngezakhiwo ezinhle kakhulu zokubopha futhi ngeke ziye kwisampula.

2. Isampula Ukuphathwa kwevolumu

Kubalulekile ukuphatha inani lesampula yoketshezi elihlobene nosayizi we-vial:

Izinga lokugcwalisa elifanele: Ngokufanelekile, uketshezi kufanele uhlale cishe ngo-10-50% wevolumu ye-vial ukuhlinzeka ngekhanda ezanele yesampula yegesi ngenkathi ugwema ukuthintana ngokweqile ngezindonga ze-vial.

3. Ukulawulwa kokushisa ngesikhathi sokuhlaziywa

Ukushisa kudlala indima ebalulekile ekutholeni imiphumela efanelekile:

Ukushisa okuqhubekayo: Gcina izinga lokushisa elihlala njalo ngesikhathi senqubo yokulingana ukuze uqinisekise ukuthi ama-Vocs afinyelela ekulingweni ngokuphumelelayo. Ukuhlukahluka ngesikhathi sokuhlaziywa kungadala ushintsho olukhulu ezindaweni eziphakeme.

4. Ukulinganisa okujwayelekile nokulungiswa

Ukulinganisa okujwayelekile kanye nokunakekelwa kwama-vials asampula kanye nezinsimbi zokuhlaziya kubalulekile:

Hlola ubuqotho be-vial: Hlola njalo ama-vials wokuqhekeka noma amaphutha angathinta imiphumela.

Ukulinganisa ithuluzi: Qinisekisa ukuthi uhlelo lwe-GC lulinganiselwe ngokuya ngemininingwane yomenzi wokugcina ukunemba kwesikhathi eside.

Ukugcina

Ukubaluleka kwe1.5 ml gc vialsEkuhlaziyweni kwesampula akunakweqiwa. Usayizi wayo ofanele usiza isampula esebenza ngempumelelo ye-HeadSpace ngenkathi unciphisa imiphumela ye-adsorption, okwenza ilungele izinhlelo eziningi zezicelo ekuqapheni kwezemvelo ekuhlolweni kwezemvelo ekuhlolweni kwemithi. Ngokunamathela emikhubeni engcono kakhulu emasu aphansi, ukuphathwa kwevolumu, ukulawulwa kokushisa, kanye nokulungiswa okujwayelekile, ama-laboratories angaqinisekisa imiphumela yokuhlaziya esezingeni eliphakeme ehlangabezana nezindinganiso eziqinile zokulawula.

WIntuthwane yokwazi okwengeziwe ngomehluko phakathi kwe-LC-MS ne-GC-MS, sicela uhlole le ndatshana: Uyini umehluko phakathi kwe-LC-MS ne-GC-MS?