Ukusetshenziswa kwe-GC-MS ekuhlolweni kokuphepha kokudla

I-Gesi Chromatography-Mass Spectrometry (GC-MS) iyindlela enamandla yokuhlaziya esetshenziswa kabanzi ekuhlolweni kokuphepha kokudla. Indlela ihlanganisa amakhono okuhlukanisa ngokomzimba we-chromatography kaphethiloli ngamakhono wokuhlaziya ngobuningi be-mass spectrometry ukunika amandla ukuhlaziya okuningiliziwe kwama-matrices ayinkimbinkimbi. Okulandelayo ukubuka konke ukubaluleka, izinhlelo zokusebenza, kanye nezinzuzo ze-GC-MS ekuqinisekiseni ukuphepha kokudla.

I-GC-MS ibalulekile ekuhlonzeni nasekunciphiseni ukungcola kanye nezinsalela ekudleni. Ukuzwela okuphezulu nokukhetha kwayo kwenza kufanelekile ngokuhlaziya ama-polarity aphansi, ama-compaile, kanye namakhompiyutha aqinile, ajwayelekile ezindabeni zokuphepha kokudla. Le ndlela ibalulekile ekutholeni izinto eziyingozi njenge-pesticides, izinsimbi ezisindayo, nezinye izinto ezingcolile ezingase zibe yingozi empilweni yabathengi.

Ngolwazi oluthe xaxa ngama-autosampler vials we-chromatography yegesi, bhekani le ndatshana: I-2ml autosampler vials yegesi ye-chromatography

Ukusetshenziswa kwe-GC-MS ekuphepheni kokudla

1. Ukuhlaziywa kwezinsalela zokubulala izinambuzane: Enye yezicelo eziphambili ze-GC-MS ekuphepheni kokudla okutholwa kwezinsalela zokubulala izinambuzane. Ngokukhathazeka okukhulayo ngemiphumela yezibulala-zinambuzane empilweni yabantu kanye nemvelo, izinhlangano zokulawula zidinga ukuhlolwa okunzima ukuze kuqinisekiswe ukuthi ukudla kuhlangabezana namazinga okuphepha. I-GC-MS iyakwazi ukukhomba izinsalela eziningi zokubulala izinambuzane ngasikhathi sinye, okwenza kube yindlela ephumelelayo yokuhlaziywa kwelebhu esebenza ngokuhlaziywa okuphelele.

2. Ukutholwa okungcolisayo: I-GC-MS isetshenziselwa ukuhlaziya ukungcola okuhlukahlukene ekudleni, kufaka phakathi ama-Mycotoxins, amakhemikhali wezimboni, kanye nokungcoliswa kwemvelo. Le ndlela iyakwazi ukuhlukanisa izingxube eziyinkimbinkimbi, ngakho-ke zilandelwa amanani engcosana angahle akwazi ukubonwa kungatholwa. Lokhu kunamandla kubalulekile ekuqinisekiseni ukuthi imikhiqizo yokudla ayinazo izinto eziyingozi nokuvikela impilo yomphakathi.

I-3. Ukunambitheka nokuhlaziywa kwephunga: Ngaphezu kokuhlolwa kwezokuphepha, i-GC-MS nayo isetshenziselwa ukunambitheka nokuhlaziywa kwephunga lokudla. Ngokuhlaziya amakhompiyutha aguqukayo abhekele ukunambitheka nephunga, abakhiqizi bangathuthukisa ikhwalithi yomkhiqizo kanye nesikhalo somthengi. Lolu hlelo lokusebenza luqokomisa ukuguquguquka kwama-GC-MS, okungagcini ezindabeni zokuphepha.

4. Ukuhlaziywa kokudla okunempilo: I-GC-MS ingasetshenziselwa ukuhlaziya okuqukethwe okunempilo kokudla, njengama-fatty acids namavithamini. Lolu hlelo lokusebenza lubaluleke kakhulu ekuqinisekiseni izimangalo zokudla okunempilo kwabakhiqizi. Ukulebula okunembile okunempilo kusiza abathengi ukuba basebenzise izinqumo zokudla ezinolwazi.

I-5. Ukulawulwa kwekhwalithi: Ngaphezu kokuthola izinto ezingcolile, i-GC-MS idlala indima ebalulekile kwinqubo yokulawulwa kwekhwalithi embonini yokudla. Ngokuqinisekisa ukuvumelana ngamaphrofayili we-flavour kanye nokwakheka kwezithako, abakhiqizi bangagcina amazinga aphezulu emikhiqizo yabo.

Izinzuzo zokusebenzisa i-GC-MS yokuhlolwa kokudla

Ukuzwela okuphezulu nokukhetha: I-GC-MS ingathola amakhompiyutha ekugxilweni okuphansi kakhulu (izingxenye ngebhiliyoni ngalinye), okwenza kube yinto elungele ukuhlela ukulandelela ukulandelela umkhondo.

Ukuhlaziywa okuphelele: Inhlanganisela yegesi i-chromatography kanye ne-mass spectrometry ingenza ukuhlaziya okusebenzayo nokunakwa kwamasampula ayinkimbinkimbi.

Ukuguquguquka: I-GC-MS ingakwazi ukuhlaziya amakhompiyutha ahlukahlukene ezinhlobonhlobo zokudla, kufaka phakathi ama-solids, uketshezi, kanye namagesi.

Imiphumela esheshayo: Ubuchwepheshe buhlinzeka ngemiphumela yokuhlaziya ngokushesha, okubalulekile ukuze ulondoloze amazinga okuphepha kokudla embonini esheshayo.

Ufuna ukwazi kabanzi ngomehluko phakathi kwe-LC-MS ne-GC-MS, sicela uhlole le ndatshana:Uyini umehluko phakathi kwe-LC-MS ne-GC-MS?

Izindlela zokuphepha ze-GC-MS ekuhlolweni kokudla

Ukuze uthuthukise ngempumelelo ukunemba kokutholwa kokudla kwe-GC-MS kwezithako zokudla, ukuhlaziywa kwenziwa ngokuya ngesimo sangempela sohlelo lokusebenza.

Okokuqala, baqonde ngokuphelele izici zamasampula okudla okufanele ahlolwe, futhi ngokwesayensi nangohlelo lokusebenza lwe-GC-MS. Ngasikhathi sinye, yakha inqubo ephelele yokubona kanye nenqubo yokusebenza, futhi yenze umsebenzi wokulungiselela ukuthola nendlela ethile yesicelo se-chromatography yegesi. Ukulawula ngempumelelo futhi wehlise izinto ezahlukahlukene ezingazinzile esigabeni sokuhlola ukuvikela umthelela emiphumeleni yokuthola ukudla futhi uqinisekise ukuthi ukunemba kwesicelo se-GC-MS ekutholweni kokudla kuyathuthukiswa.

Okwesibili, setha ngokucophelela amapharamitha wegesi ye-chromatography-mass spectrometry imishini. Isibonelo: Ukusetwa kokushisa kwebhokisi lekholomu, ukukhethwa komtshina, ukukhethwa kwekholomu ye-chromatographic, njll. Kufanele ahlangabezane nezidingo zethuluzi lokuhlola. Lungiselela imvelo ephelele yokuhlolwa kokudla ukuze uqinisekise ukusebenziseka kanye nokwenziwa kokukhethwa kwensimbi. Ngaphambi kokuba abasebenzi bokuhlola basebenzise i-GC-MS Ithuluzi, kufanele bahlole ukunemba kwemishini futhi. Qhathanisa izivivinyo zokuhlola bese ubahlaziya ukuze uqinisekise ukuthi ukuhlolwa kokudla kuhlangabezana nezidingo zegesi ye-chromatography-mass spectrometry technology.

I-GC-MS isiphenduke ubuchwepheshe be-Cornerstone ngokuhlolwa kokuphepha kokudla ngokuzwela kwayo okungenakuqhathaniswa, ukuguquguquka okuhlukahlukene, kanye nekhono lokwenza ukuhlaziya okuphelele kwezinhlobonhlobo zokungcola ezahlukahlukene. Ngokuthuthuka okuqhubekayo kwezidingo zokulawula kanye nokulindelwe kwabathengi ngokudla okuphephile, indima ye-GC-MS embonini ingahle futhi yandise. Ngokwamukela lokhu kubuchwepheshe bokuhlaziya okuthuthukile, ama-laboratories angasiza ekuqinisekiseni ukuthi ukudla kuphephile ukudla ngenkathi kugcinwa amazinga aphezulu.