E-China, inani elikhulu lemikhiqizo yezolimo (eminye imikhiqizo yamakhemikhali, ama-asidi ephilayo, njll.) idinga ukutholwa nge-chromatography ewuketshezi kanye ne-gas chromatography njalo ngonyaka. Ngenxa yenani elikhulu lamasampula, inani elikhuluamabhodlela esampula e-chromatographic idinga ukuhlanzwa phakathi nenqubo yokutholwa, okungachithi nje isikhathi kuphela futhi kunciphise ukusebenza kahle, kodwa futhi ngezinye izikhathi kubangela ukuphambuka kwemiphumela yokuhlolwa ngenxa yokuntuleka kokuhlanzeka kwamabhodlela esampula e-chromatographic ngemva kokuhlanza.

Ukuhlanzwa kwamabhodlela esampula e-chromatographic kubaluleke kakhulu. Ngokwendlela yokugeza yensimbi yengilazi, khetha indlela yokuhlanza ngokwezinga lokungcola, ayikho imodi ehleliwe. Isifinyezo sendlela:

Isixazululo 1:

1. Thela futhi womise uketshezi ku-Ibhodlela lesampula le-chromatographic.

2. Thela ngemva kwalokhu njengoba utshwala bungena kalula ku-vial engu-1.5mL futhi buncibilika nezincibilikizeli eziningi eziphilayo.

3. Thela emanzini bese ugeza nge-ultrasonic kabili.

4. Thela uketshezi ebhodleleni bese ubhaka ku-110 ° c amahora angu-1 ~ 2. Ungabhaki ezingeni lokushisa eliphezulu.
5. Cool futhi ulondoloze

Uhlelo 2:

1. Hlanza amanzi ompompi izikhathi eziningana

2. Wafake ebhekhani eligcwele amanzi ahlanzekile kanye ne-ultrasound imizuzu engu-15

3. Shintsha amanzi kanye ne-ultrasound eminye imizuzu eyi-15

4. Cwilisa kubhika nge-ethanol noma ngaphandle kwayo

5. Khipha i-zui bese womisa umoya

Uyafuna ukwazi ukuthi ungahluka kanjani izitsha ze-HPLC?, Bheka lesi sihloko:I-Crimp Vial vs. Snap Vial vs. Screw Cap Vial, Ungakhetha Kanjani?

Isixazululo 3:

1. Gcoba nge-methanol (i-chromatographic pure) futhi uhlanze nge-ultrasonic imizuzu engu-20, bese uthela i-methanol eyomile.

2. Gcwalisa ibhodlela lesampula le-chromatographic ngamanzi, hlambulula nge-ultrasonic imizuzu engu-20, bese ukhipha amanzi.

3. Ngemva kokumisaizitsha ze-hplc

Isixazululo 4:

1. Ngokuvamile ukusetshenziswa kokuqala kwamanzi ahlanzekile hashaza okomisa bese usebenzisa i-sulfate dichromate potassium lotion immersion

2. Indlela yokugeza yebhodlela lesampula le-chromatographic iyafana naleyo yesigaba soketshezi. Okokuqala, utshwala bezokwelapha busetshenziselwa ukucwilisa amahora angaphezu kwama-4, bese kuba yi-ultrasonic isigamu sehora

Isixazululo 5:

1. Uma izindleko zanele, sebenzisa i-zui entsha njalo

2. uma ufuna ukusebenzisa kabusha, indlela yokuhlanza nayo ibaluleke kakhulu, okokuqala ngesisombululo esinamandla sokuhlanza i-oxidation (i-potassium dichromate) cwilisa amahora angu-24, bese ngamanzi ahlanjululwe ngaphansi kwesimo se-ultrasonic izikhathi ezintathu, emva kwe-zui ngokuhlanza i-methanol, ukomisa kungasetshenziswa.

3. I-MATS yebhodlela kufanele ishintshwe kufakwe amasha, ikakhulukazi uma kuhlaziywa izinsalela zezibulala-zinambuzane, ngaphandle kwalokho imiphumela yobuningi izothinteka.

Wan ukwazi ulwazi olugcwele mayelana nendlela yokuhlanza izitsha zesampula ze-chromatography, sicela uhlole lesi sihloko:Isebenza kahle ! Izindlela ezi-5 zokuhlanza izitsha zesampula ze-chromatography

Okungenhla izindlela ezinhlanu zokuhlanza amabhodlela esampula e-chromatographic, sithemba ukuthi singanaka ukuhlanzwa kwezitsha ze-hplc.

Okwedlule:

Ichromatograph isebenzisa izitsha ezingenalutho kanye nesihlungi sesirinji se-HPLC

Okulandelayo:

Ungapakisha kanjani i-20ml ye-chromatograph vial

AMA-TAGS: I-hplc-autosampler-vials 2ml-gc-ibhodlela 2ml-isampula-izitsha ibhodlela - 1.5 ml

Uphenyo