I-Chromatography ephezulu yokusebenza kwe-chromatography (HPLC) kanye ne-Ion Chromatography (IC) yizo zombili izindlela ezisetshenziselwa ukuhlukanisa futhi zihlaziye amakhompiyutha kwingxube, kepha zihlukile kakhulu ezimweni zazo, izinhlelo zokusebenza, kanye nezinhlobo zokuhlaziya zilungele kakhulu ukuhlaziya.

Ufuna ukwazi izimpendulo ezingama-50 mayelana ne-HPLC VIALS, sicela uhlole le ndatshana:Imibuzo evame ukubuzwa kaningi ku-HPLC VIALS

Indlela yokuhlukanisa

I-HPLC isebenzisa ukusebenzisana kwe-hydrophobic noma i-hydrophilic yokwahlukana. Ingahlukanisa uhla olubanzi lwezinhlanganisela ze-organic, zombili i-polar ne-non-polar, isebenzisa izinhlobo ezahlukahlukene zezigaba zokumisa kanye nezinto eziphilayo eziphilayo njengama-excents. Ukuhlukaniswa kuncike ekuhlolweni okuhlobene kokuhlaziywa kwesigaba seminyaka, esingakhokhwa ngokushintshana ngeziqu ukwenza kahle ukulungiswa.

I-Ic, ngakolunye uhlangothi, ngokuyinhloko isebenzisa izindlela zokushintshana kwe-ion ukuze zihlukanise amakhompiyutha e-ionic ne-polar. Isigaba esimisiwe ngokuvamile sakhiwa ngama-ion-ukushintshaniswa ama-resins, avumela ukuhlukaniswa okusebenzayo ko-anions namazabelo. I-IC isebenzisa ama-equants alinganayo, evame ukuhlanganisa amanzi e-ultrapure anosawoti oncibilikisiwe noma ama-acid, kanye nokuhlukaniswa okuningi kwenziwa ngokuzenzelwa ngaphandle kwesidingo sokuqedwa kwezidingo.

Izinhlobo ze-Analyte

I-HPLC iyasebenziseka kalula futhi ingaphatha i-spectrum ebanzi yamakhompiyutha ezi-organic, okwenza ilungele izinhlelo zokusebenza kumakhamishini, ukudla kanye nokuhlaziywa kwemvelo. Kodwa-ke, inemikhawulo maqondana nokuhlukanisa izinhlobo ze-Ionic, njenge-anions ejwayelekile namazabelo, ezivame ukugcinwa ngempumelelo kwikholomu ye-HPLC.

IChelela i-ICELLLS ekuhlaziyeni izinhlobo ze-Ionic ne-polar, okwenza kube yindlela ekhethwayo yokuthola ama-anions ajwayelekile (njenge-chloride ne-fluoride) kanye namazwe. Kuwusizo ikakhulukazi emikhakheni efana nokuqapha imvelo, ukuphepha kokudla, nokuhlaziywa kwemithi, lapho amakhompiyutha e-ionic agcwele khona. I-Ic ingaphinde ithole amakhompiyutha angewona ama-ionic polar lapho kusetshenziswa ikholomu efanelekile nesigaba seselula.

Wazi kangakanani nge-HPLC ye-HPLC ne-HPLC elungiselelwe? Sicela ufunde le ndatshana ukuze ufunde kabanzi:Uyini umehluko phakathi kwe-HPLC yokuhlaziya neyokulungiselela?

Izindlela zokutholwa

I-HPLC ivame ukusebenzisa imishini ye-UV, edinga ukuthi ahlaziywe amukele ukukhanya kwe-UV. Lokhu kuveza izinselelo zokuthola amakhompiyutha athile, ikakhulukazi lezo ezingamukeli ukukhanya kwe-UV, njengamanye ama-ion nama-organic acid.

I-IC imvamisa isebenzisa ukutholwa kokusebenza, okusebenzayo kwezinhlobo ze-ionic. Indlela ingathola ukuzwela okuphezulu ngokusebenzisa amasu afana nokucindezelwa kwamakhemikhali, okunciphisa ukuthuthuka kwangemuva, okuvumela ukutholwa kwamazinga okuhlaziya.

Izinja

I-HPLC isetshenziswa kabanzi ekuhlaziyweni kwezingxube ze-organic eziyinkimbinkimbi, kufaka phakathi amakhemisi, izithasiselo zokudla kanye namasampula ezemvelo. Amandla awo okuhlukanisa amakhompiyutha afanayo okwenza kube yithuluzi elinamandla ezimbonini ezahlukahlukene.

I-IC inenzuzo ikakhulukazi kwizicelo ezidinga ukuhlaziywa kwezinhlobo ze-ionic, njengokuhlolwa kwekhwalithi yamanzi, ukuhlaziya ukudla, nokuhlolwa kokuhlanzeka kwemithi. Ngokuvamile kusetshenziselwa ukulekelela i-HPLC, ukunwebisa amakhono okuhlaziya ama-laboratories ukufaka phakathi uhla olubanzi lokuhlaziywa kwe-ionic kanye ne-polar.

Ngamafuphi, ukukhetha phakathi kwe-HPLC ne-IC kuncike kakhulu kwizidingo ezithile zokuhlaziywa, kufaka phakathi uhlobo lwamakhompiyutha olufundwayo kanye nokuzwela nokuxazululwa okudingekayo. I-HPLCIlungele ukwahlukana okuhlukahlukene kwezinto eziphilayo, ngenkathi i-IC iyindlela yokuzikhethela yokuhlaziywa kwe-ionic ne-polar izinhlobo.

Wazi kahle kangakanani izinyathelo zokupheka ama-chromatography vials ukuze uhlaziywe? Funda le ndatshana ukuze ufunde kabanzi: Izinyathelo ezi-6 zokulungiselela ama-chromatography vials ukuze ahlaziywe

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