Kungani i-HPLC ibalulekile ekuhlaziyeni kwemvelo

I-Chromatography ephezulu yokusebenza i-chromatography (HPLC) iyindlela ebalulekile yokuhlaziya esetshenziswa kabanzi ekuhlaziyeni kwemvelo ukuthola, ukukala, nokubheka ukungcola kumatric ahlukahlukene njengamanzi, inhlabathi kanye nomoya. Njengokukhathazeka ngokucekelwa phansi kwemvelo okubangelwa imisebenzi yezimboni, ukufuduka kwabantu emadolobheni, kanye nemikhuba yezolimo ikhula, i-HPLC isibe isisekelo ekuqinisekiseni ukuphepha kwemvelo kanye nokuhambisana namazinga okulawula.

I-HPLC iyindlela eyinkimbinkimbi ehlukanisa izingxenye ngengxube ngokususelwa ekusebenzisaneni kwawo ngezigaba ezimile neziselula. I-HPLC ifinyelela ukulungiswa okuphezulu nokuzwela ngokusebenzisa ingcindezi yokuphoqa isampula uketshezi ngokusebenzisa ikholomu egcwele izinto zesigaba semininingwane. Lesi sici singabona amanani wokungcola wokungcola okungabonakali ngezindlela ezingathandeki.

Ufuna ukwazi kabanzi nge-HPLC Sampula Ukulungiselela, sicela ubheke le ndatshana:I-HPLC isampula izixazululo zokulungiselela imiphumela emihle kakhulu

Ukusetshenziswa okuyinhloko kwe-HPLC ekuhlaziyeni kwemvelo

1. Ukuhlolwa kwekhwalithi yamanzi

Okunye kokusetshenziswa okuyinhloko kwe-HPLC ekuhlaziyeni kwezemvelo ukuhlolwa kwekhwalithi yamanzi. Ukungcola okufana nezinsimbi ezisindayo, ama-pesticides, amakhambi, kanye namakhemikhali wokuphazamisa ama-endocrine angabeka izingozi ezinkulu zempilo yabantu nasemanzini afanayo. I-HPLC ikuvumela ukuba:

Ukutholwa kwePPLTant: I-HPLC ingakhomba futhi iveze izinto eziyingozi ekugxilweni okuphansi kakhulu kumasampula wamanzi. Isibonelo, ama-pesticides atholakala ngokuhlala atholakala ku-Runoff yezolimo angatholwa.

Ukuqapha Ukuhambisana: Ama-ejensi okulawula adinga ukuqapha njalo kwezidumbu zamanzi ukuqinisekisa ukuhambisana namazinga okuphepha. I-HPLC ihlinzeka ngemininingwane ethembekile esiza iziphathimandla ukuthi zithathe izinyathelo ezidingekayo ukuvikela impilo yomphakathi.

2. Ukuhlaziywa kwenhlabathi kokungcola kwenhlabathi

Inhlabathi kungenye i-matrix ebalulekile yokuqapha imvelo ngoba inganqwabelana ngezinhlobonhlobo zokungcola ezikhiqizwa yimikhuba yezolimo, imisebenzi yezimboni, nokulahlwa kukadoti. I-HPLC idlala indima ebalulekile kokulandelayo:

Ukukhonjwa kokungcoliswa: I-HPLC isiza ukuthi ihlole ubukhulu bokuhlaziya ngokuhlaziya izinto ezinjenge-polycyclic aromatic hydrocarbons (PAPS), ama-polychlorinated biphenlys (ama-PCB), kanye nama-pesticides kumasampula enhlabathi.

Ukulungiswa kwezindlela zokulungiswa

3. Ukuqapha kwekhwalithi yomoya

I-HPLC nayo isetshenziselwa ukuhlaziya ukungcola komoya nokubheka ikhwalithi yomoya. Izicelo zifaka:

Ukuhlolwa okuguquguqukayo kwe-Organic Compound (VOC): Ama-Vocs angamakhemikhali ayingozi akhishwe emithonjeni ehlukahlukene, kufaka phakathi upende, izixazululo nezinqubo zezimboni. I-HPLC ingahlukanisa bese ilinganisa lezi zinhlanganisela kumasampula e-air.

Ukuhlaziywa kwezinhlayiyana: Le ndlela isiza ukubona izinhlayiya eziyingozi ezingathinta impilo yokuphefumula futhi zinikele ekungcoleni komoya.

4. Ukuhlaziywa kukadoti

Isimo semfucuza sibalulekile ukuze uchithe kahle kanye nokuphinda usebenzise kabusha. I-HPLC ingakusiza:

Hlaziya imifudlana yokungcola: I-HPLC isiza ukunquma indlela efanele yokulahla ngokuhlonza izakhi eziyingozi ekungcoleni.

Qinisekisa ukulahla okuphephile: Ukulandela umthetho wokulawula kudinga ukuthi imfucumfucu ihlaziywe kahle ukunciphisa ubungozi bemvelo obuhlotshaniswa nokulahlwa okungafanele.

Ufuna ukwazi kabanzi mayelana nokusetshenziswa kwama-chromatography vials, sicela ubheke le ndatshana: Izicelo eziyi-15 zesimo se-Chromatography emikhakheni ehlukene

Izinzuzo zokusebenzisa i-HPLC yokuhlaziywa kwemvelo

Kunezinzuzo ezimbalwa ezibalulekile zokusebenzisa i-HPLC ekuhlaziyeni kwemvelo.

1. Ukuzwela okuphezulu nokunemba

I-HPLC ingabona amazinga okuvota okungcolisa, okwenza kube yithuluzi elinamandla lokuqapha ukungcola okungcolisa okungenzeka kube nemiphumela emibi yemvelo noma yezempilo. Lokhu kuzwela okuphezulu kuvumela ukutholwa kusenesikhathi kwezingozi ezingaba khona ngaphambi kokuba zithuthukise zibe yizinkinga ezinkulu.

2. Ukushintshana

I-HPLC isibe yithuluzi eliguquguqukayo lososayensi bezemvelo ngoba lingakwazi ukuhlaziya ukuhlaziya okuhlukahlukene kumatric ahlukene (amanzi, inhlabathi, emoyeni, imfucuza). Izinhlobo ezahlukene zokuthola (i-UV-vis, i-fluorescence, i-mass spectrometry, njll.) Ingasetshenziswa ngokuya ngezidingo ezithile zokuhlaziywa.

3. Ukuqina

Ukuqina kwe-HPLC Izinhlelo zibenza bakwazi ukuphatha ama-matric ayinkimbinkimbi atholakala ngokuvamile kumasampula ezemvelo. Amasu wesampuli asampula afanele, njengokukhishwa kwesigaba esiqinile (i-SPE) kanye ne-Filtration, kungasiza abahlaziyi banciphise ukungqubuzana okungathinta imiphumela.

Ufuna ukwazi kabanzi ngezinto eziphikisayo ze-Chromatography zokuhlaziywa kwamanzi, sicela uhlole le ndatshana: Ama-Chromatogragraphy Vavials wokuhlaziywa kwekhwalithi yamanzi

I-chromatography ephezulu ye-i-chromatography emile njengethuluzi lokuhlaziya elibucayi ekuhlaziyweni kwemvelo ngenxa yokuzwela kwalo okungenakuqhathaniswa, ukuguquguquka nokuqina. Izicelo zalo zivelele kokuhlolwa kwekhwalithi yamanzi, ukuhlaziya kwenhlabathi yenhlabathi, ukuqapha kwekhwalithi yomoya, kanye nokuchithwa kwezinto ezibonakalayo - okunomthelela omkhulu - ngamunye onikela kakhulu ekuqondeni kwethu izinselelo zezempilo ezemvelo. Njengoba ukuqwashisa komhlaba wonke ngokuhambisana nokungcoliswa kwandiswa eceleni kwezidingo zokulawula ezindaweni ezihlanzekile, indima ye-HPLC izoqhubeka nokwanda emizamweni esekelayo okuhloswe ngayo ukuvikela impilo yomphakathi kanye nokugcina imvelo yezizukulwane ezizayo.