Ngabe uyazi ngempela i-chromatography ephezulu ye-chromatography?
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Ngabe uyazi ngempela i-chromatography ephezulu ye-chromatography?

Dec. 2nd, 2019
I-chromatography ephezulu ye-chromatography(I-HPLC) uhlobo lwe-chromatography lapho isigaba sokudlulisa uketshezi sidlula kukholamu yesigaba esiqinile esingaphansi kwengcindezi ephezulu. I-HPLC iyithuluzi elinamandla lokuhlaziya elisetshenziselwe ukuhlanza, ukuhlukanisa, ukukhomba, kanye nokunciphisa ingxenye ngayinye yengxube.

I-chromatography ephezulu ye-chromatographyIzisetshenziswa ziqukethe izigaba zeselula, amaphampu, ama-injectors, amakholomu wokuhlukanisa, kanye nemishini. I-Tamplex Material ihlelwa ngokupompa isigaba se-liquid (solvent) exutshwe nesampula kwikholamu eligcwele izinto zesigaba esiqinile. Ingxenye ngayinye yale ngxube ibhekana ngendlela ehlukile ezintweni ezibonakalayo, ihlukanisa izingxenye zezinto ezilandelwayo futhi iyeke kwikholamu ngezikhathi ezihlukile. Okokuqala, impahla ebopha kahle esigabeni se-liquid iyaphuma.
Okulandelayo, okuqukethwe okuhlanganisa kahle esigabeni esiqinile kuphume.
I-chromatography ephezulu ye-chromatography isetshenziselwa ukukala futhi ukuhlanza okuqukethwe kwezinto ezahlukahlukene zesampula. Njengesibonelo, ingasetshenziswa embonini yezemithi. Kulomkhakha, i-HPLC iyasiza kakhulu ekulinganiseni izinga lezidakamizwa ezisebenzayo kwezinto ezithile.

Cishe ama-90% abo bonke I-chromatography ephezulu ye-chromatography Izinhlelo zisebenzisa umtshina we-UV ukukala okuhlushwa ngokusebenzisa i-spectroscopcopy. Ukuhlushwa kutholakala ngomthetho kabhiya-we-lambert: a = ε C L. umtshina ulinganisa isikhathi sokuphikisana nesikhathi esisodwa noma ngaphezulu. Kunezinhlobo ezintathu zokuthola imishini.

Umtshina owodwa we-wavethth onesihlungi esimisiwe esinqunyelwe, umtshina okhethekayo we-wavelength nge-grating ejikelezayo kanye ne-waveledth diode-array-array-array-detector (ubaba). IBOSN inikeza ubaba ama-spectrometers okuyindlela esheshayo futhi eguquguqukayo kunazo zonke ehlukahlukene ye-spectrometer enezithombe ze-UV.

Uma kukhona umbuzo mayelana
I-chromatography ephezulu ye-chromatography, noma ufunaama-chromatography vials, Sicela uxhumane nathi manje!

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