Ukuhlanza i-HeadSpace Ukuhlanza & Ukuphinda usebenzise kabusha: Ukuhamba komsebenzi, ukuqhathanisa okubiza kakhulu kwama-vs ama-vs.

1. Isingeniso

Ama-vialspace ama-vials-ngokujwayelekile enziwe ngengilazi ye-borossilte-asetshenziswa kabanzi ku-GC kanye ne-GC-MS ukuhlaziya amakhompiyutha aguqukayo ku-headpace ungqimba. Ukubasebenzisa kabusha ngemuva kokuhlanza okufanele futhi incithakalo akugcini nje ngokunciphisa izindleko ezisebenzisekayo kepha futhi kusekela ukuqina kwelebhu.

2. Izinhlobo ze-Vial nezinhlelo zokusebenza ezifanele

• Scle-cap vials (screw-top): Kulula ukuvula \ ukuvala, kuyahambisana nama-autosamplers amaningi, futhi kufanelekile ukusetshenziswa okuphindaphindwayo ekuhlaziyweni kwe-VOC ejwayelekile.

• I-CRIMP-cap vials (Aluminium Crimp Lid + Septa): Nikeza ukubekwa uphawu kwe-Hermetic, okuvame ukusetshenziswa okukodwa ngoba i-crimp ibangela ukungaboni ngaso linye. Kukhethwe ngengcindezi ephezulu, ukuguquguquka okuphezulu, noma ukuhlaziya okubucayi okulawulayo (e.g. Forensic, ukudla, imithi yezemithi).

3. Ukuhlanza Ukuhamba Komsebenzi Nokususa Ukususwa Kwezinsalela

Ukulungiselela:

• Isampula eyisalayo engenalutho.

• Internet yebhodlela le-Scrub ngebhulashi noma i-scraper.

• Susa futhi uhlanze cap futhi epta ngokwahlukana.
  
  

Izinqubo ezijwayelekile zokuhlanza izinto eziningi (eziguqulwe kusuka kumaphrothokholi welebhu ashicilelwe):

Indlela A (I-General Organic Remidic)

1. Cwilisa ku-95% ethanol

2. I-Ultrasonic ihlanza kabili

3. Hlambulula kabili ngamanzi afakwe emanzini

4. Okomile kuhhavini ku- ~ 110 ° C amahora 1-2

Indlela B (Ukungcoliswa Okusekwenzi Amanzi)

1. Hlambulula ngamanzi ompompi aphindaphindwe

2. I-Ultrasonic emanzini anciphile (15min × 2)

3. Cwilisa e-anhydrous ethanol ke yomile umoya

Indlela c (Methanol-Intensive)

1. Methanol Soak + 20min Ultrasonic

2. I-Ultrasonic (20min)

3. Yomile kahle

Indlela D (i-oxidizing eqinile ehlanzekile yokungcola okunamandla)

1. Ukugeza kwe-Acid: I-Sulfuric Acid + Potassium Dichromate Sheak → Hlanza

2. Utshwala bezokwelapha suak ≥4h + 30min ultrasound

3. Ukuhlanza amanzi kwe-ultrasound → yomile

Indlela e (oxidative + ebanzi kakhulu)

1. 24h cwilisa in potassium dichromate solution

2. I-Deionized Water Ultrasonic harse (× 3)

3. I-Methanol hlambulula → umoya owomile

4. Njalo buyisela i-Septa \ Faka i-Glass Invas lapho uphinda unikeze

4. Ukwelashwa kwangaphambi kwentuthuko

Ukwehlisa izinsalela eziphansi ze-adderbed

• Ama-vials ahlanzeke ahlanze kuhhavini (110-150 ° C) amahora angu-1-2.

• Ngokuzikhethela ukuhlanza nge-inert gas noma imijikelezo ye-vacuum.

• Nweba ukulingana ngesikhathi sokufakwa kwe-GC Headspace Incubation ukusiza izinsalela ze-desb.

Lezi zinyathelo zinciphisa "iziqongo zesipoki" nomsindo wangemuva ekuhlaziyweni kwe-GC.

5. Ukuqinisekiswa nokulawulwa kwekhwalithi

• Ukuhlolwa okuseleyo: Sebenzisa ukuhlaziywa kwe-toc noma ukuqhuba imijovo engenalutho nge-GC-HS bese uqhathanisa iziqongo zangemuva kulabo abavela kuma-vials amasha ukuqinisekisa iziqongo ezingalindelekile.
  
  

• Amapharamitha wokuqinisekiswa kwendlela: Ukucacisa (ukuphindaphinda), ukugqama, izinga lokutakula (ngamazinga afihliwe), imikhawulo yokutholwa), konke kubalulekile ukuze uqinisekise ukuthi ama-vial ahlanzekile enze ngokulinganayo.

• Qc Regime: Landelela inani ngalinye le-vial lemijikelezo yokusebenzisa kabusha; Ukusetshenziswa kwemikhawulo (isb. 3-5 ukusetshenziswa). Gcina amarekhodi okuhlanza, imijovo yezikhathi ezithile engenalutho, nokuhlolwa kwe-teardown.
  
  

6. Sebenzisa kabusha impilo & izingozi

• Ekusebenzeni, ama-vials abukwayo angaphinde asetshenziswe ngokuphephilecishe izikhathi ezi-3-5Ngemuva kokuhlanza okuqinisekisiwe kanye nezinqubo ze-QC.
  
  

• Ubungozi bokusebenzisa kabusha:

  • Ukungcola okuwela

  • Ukuguqulwa kwe-septum noma ukuvuza ukuyekethisa uphawu

  • Ukulimala komhlaba ongaphezulu kwengilazi (imihuzuko, i-etching, ama-micro-fars) ukudala izicupho zokungcola

  • Ukuhlukahluka ekuhlanzeni phakathi kwama-vials nama-batches kuholela ekuzeleni okungenampilo
    
    
    

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Ngama-labs amaningi, izindleko ezifihliwe (zabasebenzi, i-QC, ama-Retrests, ama-run ehlulekile ngenxa yokungcola) ngokusetshenziswa kabusha kungahle kudluliselwe ukulondolozwa - ikakhulukazi lapho ukubonwa kwesampula nokuzwela kwezidingo.

8. Izincomo nemikhuba emihle kakhulu

• Khetha indlela yokuhlanza ngokususelwa kubukhali bokungcola kwesampula; Sebenzisa amaphrothokholi aqinile we-oxidative kuphela lapho kunesidingo.

• Njalo buyisela i-Septa; Ukusebenzisa kabusha izimbotshana \ setata kuholela ekuvuleni nasekuguqukeni.

• Sebenzisa ama-SOPs ukuhlela amabhodlela angcolile acwebile, ukulandelela Ukuphinda usebenzise, ​​kanye nokuthuthwa kwendle.

• Ngezikhathi ezithile ziqinisekisa nge-toc kanye nemijovo ye-gc engenalutho; Lahla amabhodlela kanye ne-QC ehluleka noma ngemuva kwemijikelezo yokusetshenziswa kweThreshold.

• Okwezibalo eziphakeme noma umkhondo wokuhlaziya (e.g. Pharma, i-forensic), uthande ama-vials asebenzisa ukungaguquguquki kokuvumelana nokuhambisana.

• Qeqesha abasebenzi ukuqinisekisa imisebenzi esezingeni elifanele nemisebenzi ephephile, kufaka phakathi ukusetshenziswa kwe-PPE lapho kuphathwa ama-acid nama-sol.
  
  

Ukubeka kafushane

• Okuningiliziwe, ukuhlanza okunezinyathelo eziningi kuhlangene nentuthuko ye-thermal kungahumusha ama-vials e-Glass Headspace abuyele kaningana ngaphandle kokuyekethisa okukhulu.

• Kodwa-ke, indlela ephindaphindwayo yethula ubunzima: umsebenzi, izinto zokwakha, kanye nesikhathi se-qc kungadlula imali ebilayo - ikakhulukazi ngokulandela umkhondo, olawulwa noma ama-labs anembe.

• Ukuqalisa ukusebenzisa ama-sops acacile kanye nezinqubo zokuqinisekisa kuvumela ama-labs ukulinganisa umnotho ngokuphepha, umthelela wezemvelo, kanye nekhwalithi yokuhlaziya ngokufaka kabusha ama-~ 3-5 imijikelezo ngenkathi kubhekwa engcupheni ngempumelelo.