I-Chromatography iyindlela yokuhlaziya ebabazekayo esetshenziselwa izinkambu eziningi kufaka phakathi amakhemikhali, ukuhlaziya imvelo kanye nokuhlolwa kokuphepha kokudla. Ukuzuza imiphumela eqondile nezenzekayo kubalulekile ukukhetha izingxenye ezifanele zokusetha kwakho kwe-chromatography Setup - Ukukhetha okukodwa okunganakwa ukukhetha le nqubo equkethe le nqubo ukuze izivivinyo zakho zihambe kahle ngangokunokwenzeka.

Ukuqonda indima yokufakwa okuncane

Ukufakwa okuncaneIngabe amadivaysi amancane noma ama-polymeric aklanyelwe ukulingana ngaphakathi kwentamo yezinsimbi ze-chromatography vials. Inhloso yabo ukunciphisa indawo engaphezulu phakathi kwesampula yakho kanye nengaphakathi layo ngaleyo ndlela kuncipha engcupheni yengozi yesampula ama-adsorption nokungcola.

Ukufakwa okuncane kudlala indima ebalulekile ekugcineni ubuqotho bamasampula akho. Ngokuncipha kwendawo yokuxhumana phakathi kwesampula kanye ne-vial, banciphisa amathuba okuhlaziya okunamathela ezindongeni ze-vial, ezingaholela emiphumeleni engalungile noma ukulahleka kokuthembeka kwamasampula. Ukukhetha kwakho kwezinto ezibonakalayo nokuklanywa kwe-Micro-Insert kungaba nethonya elikhulu phezu kwemiphumela yokuhlaziywa kwe-Chromatographic.

Cabanga ngevolumu yesampula yakho

Isici sokuqala lapho ukhetha ukufaka okuncane kufanele kube yivolumu yalo yesampula. Ukufakwa okuncane kuza ngosayizi abahlukahlukene kusukaI-100 ul kuya ku-400 ul; Ngakho-ke kubalulekile ukuthi noma yikuphi ukufaka okuncane okukhethiwe kwe-micro kungathola isampula yakho ngaphandle kokugcwala ngokweqile noma ukushiya i-headpace eningi kakhulu.

Ukukhetha ivolumu yokufaka i-Micro-Insert kunganciphisa imfucuza yesampula futhi kuqinisekiswe ukuhlaziya okunembile. Ukukhetha kukhulu kakhulu ukufakwa kwesampula yakho kungaholela ekugcinweni ngaphakathi kwayo; Ngenkathi ukhetha okuncane kakhulu kungasho ukuthi akunakuhlunga ngempumelelo nesampula yakho.

Khetha izinto ezifanele

Ukufakwa okuncaneWoza ngezindlela ezahlukahlukene, njengengilazi nama-polymers njenge-polypropylene ne-PTFE. Ukufakwa kwengilazi ezincane kuvame ukuba i-inert futhi kufanelekile ukuthola izicelo eziningi, yize kubuthakathaka. Ngakolunye uhlangothi, ukufakwa kwe-polymer micro-ukufakwa kuvame ukungasebenzi kahle kunezivumelwano zengilazi zazo ngenkathi unikeza ubungozi obuncane bokuphuka kodwa kungenzeka ungakunikeli ukuqina okukhulu.

Ukukhetha kwakho okubonakalayo kuncike kumasampula akho nama-sol akho; Ukufakwa kwe-micro-micro kungenzeka kube kuhle kakhulu kuma-solvents anolaka noma amasampula adinga ukuhlanzeka ngokuphelele; Ukufakwa kwe-polymer micro-ukufakwa kunikeza ezinye izindlela ezinde ezisebenza ngezindleko ezingasebenza.

Qondanisa ukufaka kwakho okuncane nge-vial yakho akuyona yonke ukufakwa okuncane okulingana nayo yonke into; Ukuqinisekisa ukuhambisana, Qondanisa ubukhulu bokufakwa kwe-micro-okuvulwa kwentamo ngokobubanzi - I-micro-ukufakwa kwenzelwe ama-vials angu-9mm noma ama-11mm ukuze asebenzise usayizi ofanele.

Ama-Mismaches phakathi kokufakwa kwe-micro-ukufakwa kanye namasayizi we-vial kungaholela ekuguqukeni kwesampula noma kungcoliswe kungcoliswe okubeka ukunemba kwemiphumela ye-chromatographic, ngakho-ke kubalulekile ukuthi uqinisekise ukuthi bayakuvikela ukuthembeka kokuhlolwa kwakho.

Cabanga ngokufakwa okukhethekile okulinganiselwe okuncane

Kunezinto ezifakwayo ezincane eziklanyelwe uhlelo lwakho lokusebenza. Isibonelo, lapho usebenza namasampula aguqukayo Kuwukuhlakanipha ukukhetha ukufakwa okufakwe ngengilazi eyakhelwe ngaphakathi noma i-PTFE Springs ukuvala izimbotshana ngokuphumelelayo nokugcina ubuqotho besampula ngaphandle kokuhwamuka lapho kuhlaziywa. Lokhu kufakwa kusiza ukuvikela ubuqotho besampula kanye nokugcina ubuqotho bokuhlaziya izinqubo zonke zokuhlaziya.

Amasampula athambekele ekuhamgisweni, khetha okufakwayo okuncane okuphakathi okuphakathi njengoba lokhu kunciphisa ukuvezwa komhlaba wesampula yakho futhi kunciphise noma iyiphi ingozi yokulahleka kwesampula kusuka ekuhambeni. Ukufakwa okukhethekile okulinganiselwe kubhekelela izinhlelo ezithile zemiphumela eqondile nethembekile kusuka kuhlolo lwakho lwe-Chromatographic.

Ukuhlolwa ukuze kuhambisane

Ngaphambi kokwenza isinqumo sakho sokugcina, kuwukuhlakanipha ukwenza izivivinyo zokuhambisana kunoma yikuphiUkufakwa okuncaneUcabanga ukuthi usebenzisa ohlelweni lwakho lwe-Chromatographic. Lokhu kufaka ukuzihlola ngokumelene namasampula akho kanye nama-sol yakho ukuze uqinisekise ukuthi awahlangani kabi noma alethe noma yikuphi ukungcola kwinqubo yakho yokuhlaziya.

Ukuhlolwa kokuhambisana kuyingxenye ehlanganisiwe yokuhlola okuphumelelayo futhi ikuvumela ukuthi ukhombe noma yiziphi izingqinamba ngaphambi kokuba ziphazamise izivivinyo zangempela. Ngokuhlola ukufakwa kwe-micro-ukufakwa ngokumelene nesicelo sakho esithile futhi uqinisekisile ukuthi ngeke kwenziwe ukuyekethisa ikhwalithi noma ukunemba kokuhlaziywa kwe-chromatographic, ukuhlolwa kokuhambisana kuyasiza ukubona noma yiziphi izingozi ezingaba khona ngaphambi kokuba zihlaziye ngokwazo.

Khetha ukufakwa kwevolumu ephansi

Lapho usebenza ngamanani wesampula alinganiselwe, ukufakwa kwe-micro-ukufakwa okuncishisiwe kwevolumu efile kungakusiza ekuhlinzekeni isithonjana sesampula esinokwethenjelwa - kuwusizo ikakhulukazi lapho uphatha amasampula ayigugu noma anqabile lapho wonke ama-cong.

Ukufakwa okuncane okuphakathizilungele izinhlelo zokusebenza lapho inani lesampula lilinganiselwe, njengalapho lisebenzelana namakhompiyutha angavamile noma abizayo. Ngokunciphisa ivolumu efile, lokhu kufakwa kwandisa ukuvuselelwa kwamasampula ngenkathi kutholakala okuningi ezinsizakalweni ezikhawulelwe.

Izinketho zokwenza ngokwezifiso

Izidingo zakho zokucwaninga ngazinye kungenzeka zingahlangane nezinketho ezijwayelekile zokufaka ezincane ezincane, futhi abanye abakhiqizi bahlinzeka ngezinsizakalo zokwenza ngokwezifiso ukuze ukufakwa okuncane kuvumelaniswe ngokuqondile ukuze uhlangane nabo.

Ukufakwa okwenziwe ngezifiso ezincane kungagxila lapho usebenza ngamasampula anzima noma izimo eziyinselele ze-chromatographic. Ukwenza ngokwezifiso kuqinisekisa ukuthi ukufakwa kwakho okuncane okuhlangabezana nakho konke ukucaciswa kwakho ngokunembile, okwenza uhlolo lwakho.

Izindleko zebhalansi nekhwalithi

Ngenkathi ikhwalithi kufanele ifike njalo kuqala lapho ukhetha ukufakwa okuncane, ukucatshangelwa kwezindleko nakho kudlala ingxenye. Hlola isabelomali sakho kanye nokusetshenziswa okulindelekile ngaphambi kokukhetha ukufakwa okuncane; Kwesinye isikhathi ukutshala imali ngokufakwa kwekhwalithi ephezulu kungahle kube nomqondo ngenkathi kutholwa amamodeli ashibhile emsebenzini wansuku zonke.

Ukulinganisa izindleko kanye nekhwalithi elabhoratri lakho kudinga ukwenza isinqumo esinolwazi ngokususelwa kwizidingo zayo ezithile kanye nezinkinga zezezimali. Ngokucabanga ngokucophelela isilinganiso sezindleko-inzuzo, ungenza izinqumo eziqinisekisa ubuqotho bokuhlola ngaphandle kwemingcele yesabelomali edlula.

Ukulungiswa okujwayelekile

Uma usuthenge ukufakwa okuncane, kubalulekile ukuthi babe yingxenye yohlelo lwakho lokulungisa lwe-labulathratory. Ukuhlanza njalo nokunakekela kuqinisekisa ukufisa nokusebenza okuqhubekayo kwalokhu okufakiwe - Ukusiza ukuvikela izingqinamba ezingalindelekile ngesikhathi sokuhlolwa.

Imikhuba yokulungisa ejwayelekile efana nokusebenzisa i-solvents efanelekile ukuze ihlanze futhi ihlole ukugqokwa nokuzikhipha izokwandisa isikhathi eside sokufakwa kwakho okuncane futhi iqinisekise ukusebenza okungaguquki ngesikhathi sokuhlaziya kwakho kwe-Chromatographic. Ngokufaka imikhuba enjalo kwimigomo yakho yelabhoratri, unganweba isikhathi sazo sokuphila ngenkathi uqinisekisa ukusebenza okungaguquki ngokuhamba kwesikhathi.

Ukugcina

Ukukhetha i-Ukufakwa okuhle okuncaneOkwakhoAma-chromatography vialskubalulekile ekuqinisekiseni ukuhlaziya okunembile nangokwethembekile. Cabanga ngezinto ezinjengomthamo wesampula, ukuhambisana kwezinto ezibonakalayo kanye nezinketho zokwenza ngokwezifiso lapho wenza lesi sinqumo sokukhetha. Ukulungiswa njalo kanye nezindlela zokulawula ezisezingeni eliphakeme zinomthelela ekuhlolweni okuphumelelayo, kusiza ukuthola imiphumela eqondile nevumayo ezindaweni zomsebenzi wesayensi noma zezimboni. Ngokwenza izinqumo ezinolwazi futhi unakisise imininingwane yokwamukela imininingwane ongayenza ukuze uthuthukise ukusetha kwakho ukuze usebenze kahle futhi uzuze imiphumela engcono kakhulu evela kuyo.

Ufuna ukuqonda okujulile ku-HPLC Vial Exernts? Hlola le ndatshana ephelele ukuthola eminye imininingwane: I-HPLC VIAL INSEPS:Ukuthuthukisa ukucacisa nobuqotho besampula

Na-

Vula izimpendulo zemibuzo evame ukubuzwa kaningi mayelana ne-HPLC VIALS kule ndaba ephelele nefundisayo: Imibuzo evame ukubuzwa kaningi ku-HPLC VIALS

Okwedlule:

Ukubuka konke kokukhethwa kwe-vial kokulungiselela amasampula amaphuzu ayi-11

Olandelayo:

Ama-Chromatogragraphy Vavials wokuhlaziywa kwekhwalithi yamanzi