I-HPLC ingenye yezindlela ezivame kakhulu zokuhlaziya okuphezulu. Injongo yayo eyinhloko: Thumela imiphumela yokuzalela futhi ethile. Isampula idinga ukulungele kahle ukujova ngqo kukholamu ye-HPLC.

Ukufeza lokhu, isampula yakho ayidingi kuphela ukuchithwa kwi-solvent efanelekile. Okubaluleke kakhulu, kumele futhi kube mahhala yezinhlayiya zokulawula ukuphazamiseka ngendlela engcono kakhulu ngesikhathi sokutholwa nokuvimbela ukuqhekeka kwekholomu yakho. Le nsangu yesampula esetshenziswayo imvamisa iyindlela eqinile edla isikhathi esidla isikhathi.

Ikhambi lesixazululo se-HPLC sesampula

Ukulungiswa kwesampula kuyingxenye ebalulekile yokuhlaziya kwe-chromatomagraphic kanye ne-spectroscopic. Le nqubo ihloselwe ukuhlinzeka isixazululo esimele, esivelisayo, kanye ne-homogenous esilungele ikholomu yokuhlaziywa kwe-chromatographic, ibe ngumthombo we-atomic adsorption, noma i-cuvete noma i-nmr tube ukuze uqhubeke. Inhloso yokulungiselela isampula ukuhlinzeka nge-aliquot eyisampula ukuthi (a) ayinakuphazanyiswa, (b) ngeke kulimaze ikholomu noma ithuluzi, futhi (c) kuyahambelana nendlela ehlosiwe yokuhlaziya.

Qhathanisa nesampula yesampula futhi engalungiselelwe

Isampula engalungiswa kahle

I-Chromatogram enomsindo wangemuva oshiwo kanye nosimende
Ikhambi lesixazululo se-HPLC sesampula

Ekuhlaziyweni kwe-HPLC, izinkinga ezivela ekulungiselelweni kwesampula zingenzeka.

1. Ukuqhekeka okungenzeka kwekholomu yakho ye-HPLC

2. Ukuzwela okuphansi kwekholomu yakho ye-HPLC

3. Peak Taling

I-5. Umsindo wangemuva ophakeme

I-6. Ama-leaable

Isampula yesampula eyenziwe kahle

I-Chromatograph enezisekelo ezizinzile neziqongo ezilinganayo

Ikhambi lesixazululo se-HPLC sesampula

Usebenzisa imikhiqizo yeSartorius ukulungiselela amasampula nge-HPLC ivimbela izinkinga ezijwayelekile ukuthi zingaveli futhi zivumela ukunemba okuphezulu kokuhlaziya.

1.NO Blockage yekholomu yakho ye-HPLC

2. Ukuzwela okuphezulu kwekholomu yakho ye-HPLC

3. Ukunemba okuphezulu

4. Bambalwa kakhulu

5. Umsindo omncane wangemuva

I-6.ANO LEACHAB

Izinyathelo ezine zokulungiselela isampula ye-HPLC

Ake sikukholise ukuthi ungasindisa kanjani isikhathi ngezixazululo zethu futhi ngasikhathi sinye ukhuphule kabusha ukuvela kwemiphumela yakho:

Isinyathelo 1. Ukuhlaziywa kwezisombululo ezinamanzi

Ikhwalithi yamanzi ampofu ingenye yezinto ezinkulu ezinikela ngezinkinga ngesikhathi se-HPLC Run. Ukungcola kwamanzi esigabeni esiseduze kungasebenzisana nekholomu futhi kuholele ekuxazululeni kabi kanye nokuzwela noma ukuzwela noma iziqongo ezingalindelekile ku-chromatograph, okuvame ukubizwa ngokuthi iziqongo zesipoki. Kusetshenziswa amanzi e-ultrapure kunciphisa kakhulu ubungozi bokungcoliswa kwezimoto eziphilayo, ama-microorganisms, endotoxins, ama-rnasus, nama-dnases. Amanzi e-Ultrapure ayatholakala ukuze athengwe, kepha, usebenzisa uhlelo lokuhlanza amanzi elebhu yakho yindlela elula yokuqinisekisa amanzi asezingeni eliphakeme. Thayipha amanzi angu-1 webanga elihlangabezana ne-ASTM, ama-NCCLS, i-ISO, kanye namazinga e-USP yizindlela ezinhle kakhulu zezinhlelo zokusebenza ze-HPLC.

Isinyathelo 2. Ukulungiselela amazinga

Ukulungiselela amazinga wokukhonjwa okuyikho kanye nokuqamba kungaba inqubo eqinile futhi edla isikhathi. Ukuhlobisa ngokunembile ukuze kusetshenziswe ivolumu elichazwe ngaphambilini linzima, futhi imfucuza ngenxa yephutha ingabiza kakhulu - ikakhulukazi lapho usebenza ngezemithi. Ezinye izinto ezinjengezinga lokushisa ziqhubeka nokulungiselela okujwayelekile. Ukushintshashintsha kwezinga lokushisa kushintsha ubunzima be-solvent futhi kufake isandla ekuhluphekeni okumangazayo kokuhlushwa.

Ikhambi lesixazululo se-HPLC sesampula

Usebenzisa ibhalansi evumela ukunemba okulinganiselwe ezindaweni eziningana zedesimali nokushintsha inqubo ngokuxhumanisa ibhalansi kwi-solvent dispenser kuqinisekisa ukucaciswa okuphezulu kakhulu. Usebenzisa isoftware efana ne-YApp16 dosing Q-App inika amandla okubalwa okuzenzakalelayo kanye nokuqapha amanani aphansi amanani alinganisiwe. Ukubhala izinga lokushisa nobukhulu be-solvent kuqinisekisa ukugxila okunembe kakhulu, ikakhulukazi lapho ulungiselela amazinga ezinsukwini ezilandelayo. Ukudluliswa kwedatha okuqondile kusuka kuhlelo lokusebenza lwe-YApp16 olunesisindo kwi-Science Science Science Science ™ Chromeleon ™ 7.2 Isoftware ye-Chromatography (CDS) I-Option (CDS) I-System (CDS) I-System (CDS) Software iyindlela yokuphatha idatha engenamaphepha enobuqotho bedatha ephelele.

Isinyathelo 3. Buyela emuva kwizisekelo nge-Pipetting enhle

Ukubaluleka kwephayiphu eqondile neqondile kuvame ukunganakwa futhi kuthathwa kalula. Izinga lekhono lomsebenzisi kanye nesipiliyoni kungaba nemiphumela ebalulekile yokuhlaziya okubucayi njenge-HPLC. Ukukhetha i-pipette efanele kunganciphisa iphutha lomuntu kanye nezindleko ezihambisana nezixazululo ezichithiwe.

Ikhambi lesixazululo se-HPLC sesampula

Ama-Pipette Menzi anokhiye wevolumu anikezela ngesisombululo esilula sokuvimbela ushintsho lwevolumu ngengozi, ngenkathi ama-Pipette e-elekthronikhi engaphezulu aqeda ukuguquguquka komsebenzisi ukuze anikeze amavolumu aqondile. Ithiphu ye-Pipette nayo iyimininingwane ebalulekile okufanele icatshangelwe yokuhlaziya i-HPLC. Amathiphu wokugcina okuphansi ahamba phambili lapho usebenza ngama-sol of viscosities ahlukene, njengoba andisa ukululama kwesampula. Ukungcoliswa kwesampula kungenzeka futhi ngenxa yokuvutha kusuka ku-pipette tip. Kanjalo, amathiphu anokuphikiswa okuphezulu kwamakhemikhali agcina ubuqotho besampula.

Isinyathelo 4. Hlunga ngokunemba nokuphila isikhathi eside

Ukuthatha ukunakekelwa okwengeziwe kokuhlunga amasampula wokuhlunga ngaphambi kokuhlaziywa kuthuthukisa ukwethenjwa futhi kunganweba impilo yekholomu ye-HPLC ngokuvimbela imiphumela yokuvala. Izihlungi zesirinji ziyikhambi elilula, elisheshayo lamasampula wokuhlunga ngaphambi kwekholomu ye-chromatography. Njengamathiphu we-Pipette, ama-solvents asetshenziswa ku-HPLC adinga ukuhlunga okuthize ukuvikela ukuguquguquka nokungcoliswa kwesampula.

Ikhambi lesixazululo se-HPLC sesampula

I-cellulose membranes iyamelana ne-dimethyl sulfoxide (DMSO), i-ANdede, ama-ketones, ester, kanye namakhompiyutha ether. Ukuze uthole izixazululo ezinzima kakhulu, i-polyethersulfone engaqinisekisiwe (PTFO) membranes ivimbela ukuguquguquka nokungcoliswa kwesampula. Ukuhlunga isampula kudla isikhathi esidla isikhathi lapho inombolo yesampula inyuka. Amayunithi we-filtration we-multi-samp, njengohlelo lwe-Claristep® Filtration, angacubungula amasampula ngaphandle kwesirinji kanye nokulungiswa kwesampula yesampula esheshayo ngaphandle kwekhwalithi enikele.

Izinkinga ezinhlanu kufanele unake!

1

Ama-laboratories angaphansi kwengcindezi eyandayo ngokwesikhathi nezindleko, imvamisa engekho ngamanani aphezulu amasampula kodwa futhi namasampula ezinvelo ezahlukahlukene. Ukulungiswa kwesampuli yindawo lapho kungafani ukuhlukahluka phakathi kwezinhlobo zezinsampula noma izindlela zokulungiselela isampula nezinto zokwakha ezisetshenzisiwe kungathinta imiphumela etholakele. Ukuqinisekisa ukuhambisana phakathi kwezinto zokwakha kudivayisi ye-filtration kanye ne-solvent kunganciphisa izingqinamba; Isibonelo, amazinga amakhompiyutha akhishwe angaphazamisa ukutholwa kokuhlaziya.

Ikhambi lesixazululo se-HPLC sesampula

2. Kwenzekani lapho i-Prep yesampula ingahambi kahle?

Ngaphambi kokuhlaziya okuphezulu kwe-chromatography chromatography (HPLC) Izicelo, amasampula alungiswa ngezindlela ezahlukahlukene, konke okunamandla okuthonya imiphumela yokuhlaziya ngokuthinta isampula elifakwe ohlelweni. Ukulungiswa kwesampula okusebenzayo kungaholela ekwehlisweni kwempilo yekholomu, izidingo ezikhulayo zensiza yezakhi, isikhathi esingaba khona sokusebenza kwezinsimbi, ukuncishiswa kokusebenza kahle kwe-chromatogagraphic kanye nokuzala, nezinye izinkinga eziningi.

3. Ekubukeni kokuqala, ukugcwala kubonakala njengenqubo elula ...

Yize i-FILTRATION iyingxenye ebonakala incane yokuchitheka komsebenzi omkhulu, umthelela wokukhetha isihlungi esifanele ukuze isicelo esinikezwe singaba sikhulu. Isibonelo, izindlela ezingasusi udaba olwanele olwenziwayo zingaholela ekuvinjelweni kwekholomu. Ukukhetha okungafanele kwamadivayisi wokulungiselela amasampula, ngaphansi kwezimo ezithile, kukhishwe amakhompiyutha athile kwisampula, kuhlanganisa amaphutha okuhlaziya noma ukwethula amaphutha ngokulahleka kwesampula - konke kuthonya imiphumela ebonakalayo. Uma uqiniseka ukuthi okudla kwakho kwe-filltration akunamthelela kumaphutha adinga umsebenzi noma ukuphenya ngezimpaphe, uzokonga imali futhi uzinikeze ukuthula kwengqondo.

Ikhambi lesixazululo se-HPLC sesampula

4

Ukukhetha okungalungile kwe-membrane kuyinkinga eyodwa esiyibona kakhulu. Izingqinamba zingavela njengeziqongo zangaphandle ezivela kuma-extractables, angaphazamisa ukutholwa kwezintshisekelo zentshisekelo. Ukungahambelani kwabezindaba zokuhlunga ngesampula noma i-solvent ngokuya ngokumelana kwamakhemikhali akugcini nje ngokunciphiswa emiphumeleni yokuchithwa kwimidiya yokuhlunga. I-Solvents ingadala ukuvuvukala okuncane kwe-polymeric membrane izinto lapho kungahambelani ngokuphelele, nakho kungathinta ukusebenza kwe-filltration, okuholela ekuhlanzeni okungasebenzi kahle kwamasampula. Enye indlela yokubhekana nalo magazini ukuthi ngokukhetha ulwelwesi oluhambisana kabanzi nge-solvents enamanzi kanye ne-organic. Ukulungiswa kwesampula yesampula ye-HPLC ejwayelekile, izihlungi zesirinji kanye nama-syringeless aqukethe ama-mempeliose avuselelekile kuyindlela enhle yokukhetha ngenxa yokuhambisana kwabo okubanzi kwe-solvent.

Ukusetshenziswa kwedivaysi yokuhlunga enkulu kunalokho okudingekayo ukuze ivolumu elilungiselelwe kungenye indawo evamile. Ukusetshenziswa kwamadivayisi amakhulu okuhlunga kusho ukuthi amanani amancane wesampula azohlala kusihlungi, noma ngabe izinyathelo zokuphepha zithathwa ukuze zixoshwe ngangokunokwenzeka.

Ikhambi lesixazululo se-HPLC sesampula

5. Yiziphi izinzuzo zokuthatha isinyathelo sokuhlunga ngokungathi sína?

Inhloso ebalulekile ye-laboratories eminingi ebandakanyeke ekuhlolweni kokuhlaziya ukuthuthukisa izinqubo ezingaguquki ezenzekela imiphumela engaguquki - futhi edinga ukuhlunga okungaguquki. Ukukhethwa kwedivaysi ye-FILTRATION ngosayizi we-pore usayizi we-pore kanye nokugcinwa kwezimpawu kusiza ekuqinisekiseni ukususwa okusebenzayo, ngenkathi ukukhethwa kosayizi ofanele kanye nokwakhiwa kweyunithi yokuhlunga kungasiza ekunciphiseni ukulahleka kwesampula. Ukuhlunga okuhle kuyasiza ukuvikela ikholamu futhi kungandisa impilo yonke yesijolo nekholamu.

Ukugcina

I-HPLC iyindlela ebalulekile yesayensi yesayensi kanye nocwaningo lwe-biopharmaceutical njengoba ihambisa imiphumela enembile, ephindeka. Lokhu kunemba kuncike ekuthembekeni kwesampula ye-HPLC, kanye nendlela engcono kakhulu yokuqinisekisa amasampula asezingeni eliphakeme ukuqala ngamathuluzi amahle kakhulu. Ngezixazululo zamanzi e-Ultrapure, ukulungiswa okujwayelekile, ama-Pipette, amathiphu, nezihlungi, i-HPLC isampula yokulungiselela umugqa akhawunti yonke imininingwane. Izisombululo ezenzakalelayo kanye nesoftware nazo zisiza ekuhleleni ukulungiswa kwesampula ukuletha amasampula ahlanzekile emiphumeleni ehlanzekile.

Izingxoxo eziningi nge-HPLC isampula izixazululo zokulungiselela zemukelwa!

Okwedlule:

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Olandelayo:

Ukuhlunga ngezimpawu uzokusebenzisa futhi?