Izihlungi zesirinji zingamathuluzi ayisisekelo ezilungiselelweni ezihlukile zesikhungo se-Onetical and Research Center. Babalulekile ekuhleleni izivivinyo zamasu achazayo. Lezi zinqubo zifaka i-chromatography ephezulu ye-chromatography (HPLC), i-chromatography yegesi (GC), ne-spectroscopy. Lezi ziteshi zibalekela ukungcola nokunye okungcolile kusuka ekuhlolweni koketshezi. Baqinisekisa ukunemba kwemikhiqizo yakho.

Lesi sihloko esiphelele sizokuqondisa ngokusebenzisa izihlungi zesirinji. Izomboza inqubo yesinyathelo ngesinyathelo. Sizophinde sikuqoqe ukucatshangelwa okubalulekile. Gcina engqondweni lapho usebenzisa la mathuluzi asemqoka.

Kusetshenziswa kuphi izihlungi zesirinji? Funda ngezicelo zayo eziningi lapha:Ukuhlola ukusetshenziswa kwezihlungi zesirinji ze-PVDF ekuhlutjweni kwelebhu

Ungasisebenzisa kanjani izihlungi zesirinji

Izihlungi zesirinjiyakhelwe ukuthi isetshenziswe ngokubambisana nesirinji. Kusetshenziswa isihlungi esirinji kulula. Kodwa-ke, kubalulekile ukulandela izinyathelo ezifanele. Lokhu kuqinisekisa ukuhlunga okusebenzayo futhi kugweme izinkinga.

Izinyathelo zokusebenzisa izihlungi zesirinji

Isinyathelo 1: Lungiselela isampula.

Qiniseka ukuthi ixubene kahle ngaphambi kokuhlunga. Lesi sinyathelo sisiza ukuyeka izinkampani ezixazululiwe noma ezimisiwe. Babengaphazamisa ukuhlunga. Isampula elilungiselelwe kahle lizogobhoza kahle ngesihlungi se-syripha. Lokhu kunciphisa ingozi yokuvalwa kwe-clogging noma ngaphambi kwesikhathi.

Isinyathelo 2: Namathisela isihlungi esirinji.

Thatha isihlungi bese uxosha noma yiziphi izigaxa noma izigqoko. Joyina ngokucophelela isihlungi esiphethweni sesirinji, ukwenza okungaphezu kokungabaza inhlangano ephephile. Lesi sinyathelo sibucayi ukuthi silindele ukuchitheka noma ububi bokuhlola phakathi kokulungiswa kwe-filtration.

Isinyathelo 3: Dweba isampula kusirinji.

Ukudweba kancane, unakekele ukuthi ungangezi ama-bubble amoya. IVolumu yesampula kufanele ibe likhulu kunevolumu yokugcina. Lokhu kungenxa yokuthi amanye amasampula angagcinwa kusihlungi. Ukungeza ama-Bubble e-AIR kungenza amanani okugeleza akuhambisani. Kungalimaza nokuhlunga.

Yini okufanele ngiyenze uma inaliti ilimele ngesikhathi sokuhlunga kwesampula? Le ndatshana ingahle ibe lusizo:Ukubhekana nomonakalo wesirinji ngesikhathi sokusebenzisa

Isinyathelo 4: Hlunga isampula.

Bamba isirinji ngokuqondile ngesihlungi phansi. Cindezela ubumnene nge-plunger ukuze uhlunge kancane isampula ngesihlungi sesirinji. Gwema ukucindezela kanzima. Lokhu kungalimaza isihlungi futhi kulimaze ukugcwala. Ukugcina izinga lokugeleza okulawulwayo nokuqinile kubalulekile ukuze uthole ukuhlunga okusebenzayo.

Isinyathelo 5: Qoqa isampula ehlungiwe.

Beka i-vial ehlanzekile noma isitsha ngaphansi kwesihlungi esirinji. Cishe kancane i-plunger yokukhipha isampula elihlungiwe emkhunjini wokuqoqa. Lesi sinyathelo siqinisekisa ukuthi isampula elihlungiwe liqoqwe ngaphandle kokungcola okwengeziwe.

Isinyathelo 6: Lahla isihlungi.

Lapho isihlungi senziwe, susa isihlungi bese usilahla kahle. Landela imithetho yesikhungo sakho noma selebhu. Ukudluliselwa okufanelekile kwezihlungi ezisetshenziswayo kubalulekile ekuvikeleni nasekucabangeni kwemvelo.

QAPHELA: Ukucatshangelwa Okubalulekile

Lapho usebenzisa izihlungi zesirinji, gcina engqondweni izinto ezimbalwa ezibalulekile. Baqinisekisa ukugcwala okusebenzayo nokuphephile.

a.Khetha isihlungi se-syrinji ngokuya ngesampula yakho nendlela yakho. Khetha usayizi we-pore ofanele kanye nezinto ezibonakalayo. Isilinganiso se-pore kufanele sibe mancane kunokulinganisa kwezinhlayiya noma ezingcolisayo ukhomba ukuphuma. Izinto zokuhlunga ezihlukile zisebenzela izinhlobo zokuhlola ezihlukile. Zinezidingo ezihlukile zokuhambisana. Kuleli cala, izinto zokwakha zifakacellulose aceticUkususelwa ku-acid,I-PTFE, futhiinjobe.

Ungayikhetha kanjani isihlungi esifanele? Thola amathiphu wokukhetha anemininingwane eminingi lapha: Ungasikhetha kanjani isihlungi esifanele sesirinji sokulungiswa kwesampula yakho?

b.Qiniseka ukuthi ukuhlunga kuyahambelana nesampula yakho. Kufanele futhi isebenze ngama-solvents noma izixazululo ezisetshenzisiwe. Ezinye izinto zokuhlunga zingaxhumana nokuhlaziywa okuthile. Bangakwazi ama-adderb, okuholela ekulahlekelweni kwesampula noma ekungcolisweni. Kufanele ukhethe isihlungi esisebenza ngesampula yakho nokuhlaziywa.

c.Ivolumu ye-filtration. Ungangeqi ivolumu ephezulu yesihlungi sesirinji. Ukulayisha ngokweqile isihlungi kungaholela ekubolekeni ngaphambi kwesikhathi nokungasebenzi kahle. Cabanga ngevolumu yesampula ngokucophelela. Hlola umthamo wesihlungi ukuze uqinisekise ukugcwala okuhle.

d.Hlunga kancane. Ukuhlunga okusheshayo kuzwakala isihlungi kungekudala. Lungisa ingcindezi ye-plunger ukuze ulondoloze izinga lokugeleza eliqinile, elawulwa. Lokhu kusiza ukukhulisa isikhathi sokuphila kwehlungi futhi kuqinisekisa ukulungiswa kwesampula okungaguquki.

e.Usayizi wesirinji. Sebenzisa usayizi wesirinji ofanele ivolumu yesampula nosayizi wokuhlunga. Ama-syringe amakhulu anikela ngokulawula okwengeziwe kanye nenqubo yokuhlunga ebushelelezi. Ama-syringe amancane angaba ngcono kumavolumu amancane wesampula. Ukukhetha usayizi olungile wesirinji kungenza ukugcwala kube lula futhi kusebenze kakhudlwana.

Kungani abantu abaningi bekhetha isihlungi se-PTFE Syringe ukulungiselela amasampula? Lo mbhalo uzophendula imibuzo yakho:Ukwenza ngcono ukuzwela kokuhlaziya ngesihlungi se-PTFE Syringe Filtration of Trace Samples

f.Sebenzisa izihlungi ezinyumba ngamasampula ayinyumba. Sebenzisa izihlungi eziqinisekiswe ukuthi inzalo. Lokhu kugcina isampula liqinile. Lezi zihlungi zinezici ezikhethekile. Zenzelwe ukususa ama-microorganisms futhi ugcine isampula ehlungiwe.

g.Kwezinye izimo, kungahle kudingekile ukwenza izinyathelo eziningi zokuhlunga. Usebenzisa izihlungi zesirinji ezinosayizi omncane futhi omncane. Senza lokhu ukufeza izinga elifunayo lokuhlanzwa kwesampula. Le ndlela ilusizo kumasampula anenkinga eningi yodaba. Kuyasiza futhi uma kunezidingo ezithile zokuhlunga.

Ngokulandela lezi zinyathelo nemibono, ungasebenzisa izihlungi zesirinji kahle. Balungiselela amasampula akho ukuhlaziya kamuva. Lokhu kuqinisekisa ukuthi imiphumela yakho ithembekile futhi inembile.

Ukuqeqeshwa okufanele futhi kulandela imithetho yezokuphepha yeLab kubalulekile. Lokhu kuyiqiniso ikakhulukazi lapho usebenza ngokuhlunga kwesirinji kanye namanye amagiya welebhu. Hlala uhlola imihlahlandlela yesikhungo sakho. Uma unemibuzo noma izinkathazo, buza ozakwabo noma abaqondisi usizo.

Awunaso isiqiniseko sokuthi yimiphi into yokuhlunga okufanele uyikhethe? Le ndatshana izokutshela umehluko phakathi kwe-PVDF ne-Nylon:Izihlungi zesirinji ze-PVDF vs.

Okwedlule:

Qonda izilinganiso zokucindezela ze-headpace vial

Olandelayo:

Umhlahlandlela wokugcina wokuhlunga okungu-0.45 micron