Ekuhlaziyweni kwe-chromatographic,Izihlungi zesirinjiKhonza njengethuluzi elibalulekile ekulungiselweni kwesampula, uqinisekise ukuthi i-tampled ifakwe ohlelweni lweChromatographic mahhala lokungcola kanye nodaba lokubandakanya. Lezi zihlungi ngokuvamile ziqukethe izindlu ngesihlungi se-membrane futhi zikhethwe ngokususelwa ezintweni ezifana nosayizi we-pore nokufaneleka kwezinto ezibonakalayo.

Kuyini isihlungi sesirinji?

Izihlungi zesirinji zingamadivayisi amancane alahlwayo asetshenziselwa ukuhlunga izixazululo ngaphambi kokuthi zifakwe ohlelweni lwe-Chromatography. Lezi zihlungi ngokuvamile zihlanganisa izindlu ngesihlungi se-membrane ukuze zihlukanise izinhlayiya nokungcola kusuka kusampula. Ziyatholakala ngosayizi ahlukahlukene, izinto zokwakha, kanye nosayizi we-pore ukuhambisana nezicelo ezihlukile.

Izimbangela zokugcinwa kwesampula

Ukuqonda izimbangela zokugcinwa kwesampula ekuhlunga okuyisirinji kubalulekile ukubhekana nalolu daba ngempumelelo.

Usayizi weMembrane Pore

Le khasiusayizi we-pore of the membrane fingeryinto ebalulekile. Kuncane kakhulu usayizi we-pore ungaholela ekugcinweni kwe-clogging kanye nokugcinwa kwesampula. Ngakolunye uhlangothi, uma ubukhulu be-pore bukhulu kakhulu, izinhlayiya ezingafuneki zingadlula futhi zithinte ukuhlaziywa. Kubalulekile ukukhetha usayizi ofanele we-pore ngokuya ngezimpawu zesampula.

Isampula Yesampula

Ukwakheka kwesampula uqobo kungathinta kakhulu izindaba zokugcinwa. Amasampula as viscous kakhulu, izinhlambaza, noma aqukethe ama-matric ayinkimbinkimbi maningi amathuba okuba abambe ukugcinwa. Ukuqonda ukwakheka kwesampula kuzokusiza ukuthi ukhethe izindlela ezifanele ze-FILTRATION kanye namasu wokuhlekisa wokunciphisa ukugcinwa.

Ufuna imininingwane ephelele ekuhlunga kwesirinji? Ngena kule ndatshana ukuze uthole ukuqonda okuphelele nokuqonda okubalulekile:Umhlahlandlela ophelele wokuhlunga kwesirinji: Izici, Ukukhetha, Intengo, kanye nokusebenzisa

Okokuhlunga okubonakalayo

Izinto ezisetshenziselwa ukuhlunga kwesirinji nazo zithinta ukugcinwa kwesampula. Izinto ezahlukahlukene zinamazinga ahlukahlukene okuhambisana nezinhlobo ezihlukile zesampula. Isibonelo, izihlungi ze-hydrophobic zilungele amasampula angewona ama-polar ngenkathi izihlungi ze-hydrophilic zilungele amasampula wePolar. Ukukhetha okokuhlunga okufanelekile ngokusekelwe kwizici zesampula kunganciphisa ukugcinwa.

Isihlungi esimanzi

Ukuqina okufanele kweHlunga ulwelwemu lokuhlungakubalulekile ukuvikela ukunamathela kwesampula nokugcinwa. Ukumanzi okunganele kungadala ukuthi isampula linamathele endaweni yokuhlunga futhi linciphise ukusebenza kahle kwe-filltration. Usebenzisa i-solvent ehambisanayo noma isisombululo sokumanzisa isihlungi ngaphambi kokuhlunga kuzoqinisekisa ukusebenza kahle nokugcinwa okuncane.

Umthelela ekuhlaziyweni kwe-chromatographic

Ukugcinwa kwesampula kokuhlunga ngezimpawu kungaba nemiphumela emibi emincane ekuhlaziyweni kwe-chromatogagraph

Ukulahleka kokuhlaziya

Lapho amasampula egcinwe kuhlunga lwesirinji, ukuhlaziya kungalahleka, kuholele ekunciphiseni okungalungile nekhwalithi yedatha engeyinhle. Lokhu kuyinkinga ikakhulukazi lapho kuhlaziya amasampula aphansi noma umkhondo.

Thola ukuthi ukuhlunga kwesirinji kungasetshenziswa yini kule ndaba efundwayo! Thola amaqiniso ngokuphinda usebenzise izihlungi zesirinji futhi wenze izinqumo ezinolwazi:Ukuhlunga ngezimpawu uzokusebenzisa futhi?

Ukuzwela okunciphisiwe

Ukugcinwa kunganciphisa futhi ukuzwela kokuhlaziywa. Njengoba ukuhlaziya okungaphansi kufinyelela kwikholamu ye-chromatographic, amandla okuthola nokuthola amazinga aphansi aphansi ancishisiwe. Lokhu kungathinta umkhawulo wokutholwa kanye nokuzwela okuphelele kwendlela yokuhlaziya.

Ukungcoliswa Kokholamu

Izinhlayiya kanye nokungcola okugcinwe kusihlungi se-syringe kungagcina ukufudukela kwikholamu ye-chromatographic. Lokhu kungangcolisa ikholamu futhi kuthinte ukusebenza kwekholomu, ukwakheka okuphezulu, nokuqina kwesisekelo. Amakholomu angcolisiwe adinga ukulungiswa okuvame kakhulu futhi kungahle kube nomphumela wokuhlaziya okude kokuhlaziya.

Amasu wokunciphisa

Amasu amaningana angaqashwa ukubhekana nokugcinwa kwesampula ekuhlunga kwesirinji.

Lungiselela usayizi we-pore

Kubalulekile ukukhetha aisihlungi sesirinjingosayizi ofanele we-pore. Kufanele ivumele ukugcwaliswa okusebenzayo ngaphandle kokubamba ngokweqile. Ukuqonda izici zesampula kanye nokungcola okungenzeka kuzosiza ekukhetheni usayizi omuhle kakhulu we-pore for Filtration.

Ufuna ukwazi ngokubaluleka kwezihlungi ze-micron? Hlola le ndatshana efundisayo ngemininingwane ephelele nokuqonda:Igayidi ephelele yokuhlunga okungu-0.22 micron: konke okudingeka ukwazi

Ukuhlunga kwangaphambili

Kumasampula anokuqukethwe okuphezulu kwendaba noma ama-matric ayinkimbinkimbi, ukugcwala kwangaphambilini kusetshenziswa izihlungi zikasayizi ezinkulu ze-pore kunganciphisa ukugcinwa kusihlungi sesirinji sokugcina. Le nqubo yokuhlunga okunezinyathelo ezimbili ithuthukisa ukusebenza kahle kwe-fieltration ngoba izinhlayiya ezinkulu zisuswa ngaphambi kokuba isampula lidluliselwe ngolwelwesi oluhle.

Ukumanzisa okufanele

Ukwehla okufanele kolwelwesi lokuhlunga kubalulekile. Ukumanzisa isihlungi nge-solvent ehambisanayo noma isixazululo ngaphambi kokuhlunga kuvimbela ukunamathela kwesampula futhi kuthuthukise ukusebenza kwe-filltration. Ukumanzisa okufanele nakho kusiza ukugcina ubuqotho beHlunga ulwelwemu lokuhlungakuyo yonke inqubo yokuhlunga.

Ukulondolozwa Kwendlela

Ukuhlolwa njalo nokugcinwa kwezihlungi zesirinji kuyadingeka ukuqinisekisa ukusebenza kahle. Faka esikhundleni izihlungi ngokuya ngezincomo zomkhiqizi futhi ugweme ukusebenzisa izihlungi ezilahliwe ukuvikela ukungcoliswa kokunqamula kanye nezinkinga zokugcinwa. Ukulondolozwa okufanele nokuphathwa kokuhlunga kuyasebenza futhi ekunciphiseni ukugcinwa kwesampula.

Ekuphetheni,ushikukazichofoza isihlungiUkugcinwa kwesampula kuyinselelo ekuhlaziyweni kwe-Chromatographic, kepha kungabhekelwa ngempumelelo ngamasu afanele. Ngokuqonda izimbangela zokugcinwa, ukukhetha isihlungi esifanele, ukwenza kahle usayizi we-pore, ukuqinisekisa ukuqina okufanele, nokulungiswa okujwayelekile, abacwaningi banganciphisa imiphumela ethembekileyo nenembile ekuhambeni kwabo kokuhlaziya. Izihlungi zesirinji ziyithuluzi elibalulekile lokulungiselela amasampula futhi lifake isandla kwikhwalithi nobuqotho bokuhlaziywa kwe-chromatomagraphic ezinhlobonhlobo zezimboni.

Unentshisekelo yokufunda ngokubaluleka kwezihlungi ze-micron eziyi-0.45? Ngena kule ndatshana enokuqonda ukuthola imininingwane ebalulekile nokuqonda:Igayidi ephelele yokuhlunga okungu-0.45 micron: konke okudingeka ukwazi

Okwedlule:

Izakhiwo ze-Microscopic ku-Chromatogragraphy Vavials: Umthelela ongaba khona kwisitoreji sesampula nokuhlaziywa

Olandelayo:

Ukusebenza kokubekelwa uphawu okungalingani