Hauv HPLC qauv npaj ua ntej npaj, cov hmoov nplej lim dej ua lub luag haujlwm tseem ceeb. Particulates hauv cov qauv muaj peev xwm txhaws, cov plaub lub neej luv, txoj sia hauv cov cim taw qhia, thiab kev sib txawv. Kev ua kom pom kev ua ntej tshem tawm cov impurities no, txhim kho cov rov ua kom rov muaj dua thiab cov hauv paus ruaj khov. Koob tawv nqaij cov kua txiv kab ntxwv muab cov tshuaj uas yooj yim siv, kom muaj kev txhaj tshuaj, tiv thaiv ob qho ntsuas, thiab txhim kho kev tiv thaiv kev tiv thaiv kev ntseeg tau

Cov yam ntxwv tseem ceeb ntawm cov khoom lim sib txawv

PTFE (polytetrafluoroethylene) cov cai

Tshuaj sib xyaw ua ke:Tshwj xeeb inert thiab tiv taus yuav luag txhua organic cov kuab tshuaj, acids, thiab cov hauv paus, tab sis hydrophobic-tsis haum rau kev ua raws li cov a aqueasy. Qhov zoo tagnrho rau cov kuab-polarity (piv txwv li, acetoniturile, dmo), siab-kub, thiab kev xeb ib puag ncig. Tso tawm zoo rau tsis muaj Extractables.
Txaus tus nqi:Tus qauv tuab yuav tsum tau ua ntej ntub nrog organic kuab tshuaj, ua rau cov nqi qis dua.
Cov khoom muaj protein: Hydrophobic saum yields minimal tsis yog-tshwj xeeb protein adsorption, ua rau nws zoo tagnrho rau kev ntsuas qib theem.Siv cov xwm txheej & txwv:Qhov zoo tshaj plaws rau kev ua phem lossis ua kom zoo nkauj polar (acid digests, reagent extracts). Tsis yog rau dej yam tsis muaj ua ntej ntub; Zam kev ua yeeb yaj kiab ziab kom tiv thaiv lub kua muag.

Nylon wembranes
Tshuaj sib xyaw ua ke:Hydrophilic; Tau tshaj cov dej thiab feem ntau cov kuab tshuaj organic. Cov kuab tshuaj uas zoo li acetoniturile thiab DMOPS tau txais kev txhawb nqa tag nrho, thaum thf thiab siab-siab Methanol yuav ceeb toom kom ceev faj. Stability tsis lees nyob rau hauv muaj zog acids \ / bases.Txaus tus nqi: Nruab Nrab-Wets sai thiab lim ceev dua li PTFE, tab sis feem ntau qeeb dua PVDF.Cov khoom muaj protein:Cov siab tsis zoo-adsorption-tsis haum rau cov tshuaj tiv thaiv protein-nplua nuj xav tau qhov siab tshaj plaws. Siv cov xwm txheej & txwv: Versatile rau kev niaj hnub organic kuab tshuaj lossis ntas cov qauv aqueous (piv txwv li, acetonitrile, methanol). Rugged thiab autoclavable, tab sis zam cov protein lossis muaj zog-acid \ / paus pib kuaj.

PVDF (polyvinylidene fluoride)
Tshuaj sib xyaw ua ke:Hydrophilic; ruaj khov nrog dej, cawv, thiab ketones nyob rau hauv nruab nrab kom me acidic \ id acidic \ / yooj yim tej yam kev mob. Heev UV tshem tawm tsawg zaus ntawm kev cuam tshuam ntawm yav dhau los.Txaus tus nqi: High-wets yooj yim, muab zais kom nrawm dua li PTFE ntawm tib lub pore lojCov khoom muaj protein:Tsawg-zoo heev rau cov piv txwv biological, cov protein, thiab kev coj ua xov tooj ntawm tes.
Siv cov xwm txheej & txwv:Qhov zoo tagnrho rau cov protein \ / peptide lossis dej cawv ua piv txwv. Nkag siab txog cov hauv paus muaj zog, uas tuaj yeem ua rau cov mem caj qaum.

PC (Polycarbonate) WhitBranes
Tshuaj sib xyaw ua ke: Du, radust nto tau tshaj nrog ntau cov acids, ntsev, roj, thiab cov roj khov. Tsis tiv taus methanol, ketones, neeg phem, lossis aromatics.Txaus tus nqi:High-uniform high-uniform pore faib yields qis rapperture thiab ntws sai.Cov khoom muaj protein:Tsis tshua muaj-iav-zoo li nto txwv tsis pub tshaj tawm cov protein thiab molecules loj.
Siv cov xwm txheej & txwv:Haum rau particle suav, microschopic partic particate, thiab cell kev tshawb fawb xav tau kom ceev. Tsis tshua muaj siv nyob rau hauv kev niaj hnub organic HPLC vim yog cov kuab tshuaj txwv.

Kev sib piv sib piv

Daim nyias nyias	Tshuaj Muaj Tshuaj Ntau	Txaus tus nqi	Cov tshuaj tiv thaiv muaj protein	Cov Ntawv Thov & Txwv
Ptfe	Tiv taus yuav luag txhua cov kuab tshuaj xeb; hydrophobic	Qi	Tsawg heev	Hnyav ua theem theem; Tsis yog rau aqueous yam tsis muaj ua ntej ntub
Nylon	Hydrophilic; zoo nrog ACN, DMO; Ceev faj nrog thf, meoh	Tswj kav	Siab	Cov organics niaj hnub \ / buffers; Zam kev tawm dag zog & cov kua qaub muaj zog
Pvdf	Hydrophilic; ruaj khov nrog dej, cawv, ketones	Siab	Qi	Kev faib tawm muaj kev haus roj ntsha \ /; Zam kev ua haujlwm ruaj khov
PC	Du; zoo nrog kua qaub, ntsev, roj; tsis rau meoh, ketones	Siab heev	Tsawg heev	Particle \ / microscopy; Tsis tas li cov organic HPLC

Xaiv tus cwj pwm zoo rau koj cov qauv

Hnyav los yog qib siab polvents + trace kev tsom xam: Kev sivPtfeRau nws cov tshuaj tsis muaj kev tsis txheeb thiab cov extractables tsawg.

Kev ntsuas kev ua piv txwv (cov protein) + cawv \ / dej: Kev sivPvdfRau qhov tsawg kawg protein khi thiab daws cov daws teeb meem sib xyaw.
Niaj hnub cov organic kuab tshuaj:Kev sivNylonRau nws txoj kev sib haum xeeb thiab kev siv zog tsis tu ncua-zam kev ua protein lossis ntau.
Particle suav \ / microscopy: Kev sivPCTxog kho pore loj thiab negrigible adsorption.

Nco tseg:Nco ntsoov xav txog cov qauv chemistry, tus nqi, thiab cov cai ua kom cov kev xav tau thaum ua koj txoj kev xaiv zaum kawg.

Aijiren muaj tag nrho ntawm cov hmoov nplej zoo siab nrog PTFE, nylon, PVDF, thiab PC Whemranes kom tau raws li koj cov HPLC cov organic-theem xav tau. Tiv tauj peb rau cov qauv lossis kev sab laj kev tswv tsev, thiab peb pab peb xaiv qhov kev daws kev lim lim zoo.

Yav dhau los:

Cov tshuaj uas tsis muaj txiaj ntsig zoo, vial tej kev kho, cov vially vials, peg coated vials, pfdcs txheej

Tom ntej:

Tsis tshua muaj siab heev \ / tsawg kub thiab lub teeb kis cuam tshuam rau cov qauv ntawm cov qauv ruaj khov: Theory & Methodolog