Cov tshuaj uas tsis muaj txiaj ntsig zoo, vial tej kev kho, cov vially vials, peg coated vials, pfdcs txheej

Nyob rau hauv high-rhiab kev tshuaj xyuas, adsorption kev poob ntawm Vial Surfaces tuaj yeem txwv pom qhov raug kom raug. Intrinsic Silanol pab pawg (Si-Oh) thiab trace hlau sib txuas lus nrog cov qauv molecules, immobilizing polraculizing polar lossis foob ntawm lub vial phab ntsa. Tsis muaj kev tsis kho cov tshuaj feem ntau yog cov tshuaj rov qab zoo dua 80%, thiab cov qauv kev ua haujlwm tsis muaj qhov tseem ceeb ntawm kev thim rov qab. Cov neeg muag khoom pom zoo kom cov iav vials rau cov kab mob siab polar, thiab cov qauv tshawb nrhiav pom tias muaj teeb meem ppb-teeb liab tsis pub dhau lub ntsej muag tsis pub dhau lub sijhawm. Yog li ntawd, qhov chaw passive los yog txheej yog qhov tseem ceeb rau txoj kab-theem raug.

2. Khob Lub Chaw Ua Si thiab Adsorption Mechisms

a. Silanol Pawg thiab Hlau Is
  Kuv. Nto Si-Huag Pawg Bixar Ponar Analytes
  ii. Lw Hlau Ions Compressoratic Sib Tham nrog them cov qauv txiav nyiaj

b. Hnyav poob siab
  Kuv. Organic kuab tshuaj (piv txwv li, Acn, Meoh) tuaj yeem txwv passivation txheej, qhia cov chaw tshiab

c. Kev nqa khoom muaj paug
  Kuv. Residual raug them lossis hydrophilic lwg ntawm phab ntsa tsim yeeb pom dab tsi hauv cov kab tom ntej

d. Caw Kaw Lus Siv
  Kuv. Rov qab txhaj rau hauv high-topputic system nce ntaiv ntawm polar lossis lw analytes
  ii. Kev tshaj tawm teeb liab feem ntau tshaj 10% dhau sijhawm

3. Cov ntsiab lus saum npoo: Deactivation thiab Txheej

3.1 Kev Tso Cai Tso Cai

a. High-kub tua hluav taws (~ 800 ° C)
  Kuv. Cleavoles qee si-huag tab sis nplooj hlau ions lawm

b. Acid Ntxuav (E.g., 6 M Hcl)
  Kuv. Tshem tawm cov hlau ions tab sis rougens iav saum npoo

c. Puag ntxuav (piv txwv li, 1 m naoh)
  Kuv. Tsim cov si-o⁻, counterproductive

d. Kev txwv
  Kuv. Tsuas yog them ib nrab ntawm cov chaw nquag ntawm iav substrate

3.2 Silanization

a. Cov orgososilane kho nyob rau hauv lub tshuab nqus tsev
  Kuv. Organosilanes (E.G., Methylsilane) Daim Ntawv Cog Lus Si-O-Si-Si-Si-Si nrog Deg Silanols
  ii. Tsim cov thaiv kev hydrophobic uas tiv thaiv kom kub, acids, thiab cov hauv paus
  III. Lowers qis sab nro nro thiab kho kom rov zoo li polar artalyte rov qab mus rau 90%

b. Muag khoom ua piv txwv
  Kuv. "DV" cov vials silanized rau polar-compound tsom xam (dej)

3.3 Txheej Txheem Txheej Txheem

a. Perfluorodecylrichylorosilane (PFDCS)
  Kuv. Tus Kheej Ua Tus Kheej Monolayer Yield Superhydrophobic Nto
  ii. Qhov zoo tagnrho rau Nonparar Pats thiab Lipid-soluble paug

b. Polyethylene Glycol (Peg)
  Kuv. Hydrophilic saw hluav taws protein, peptides, thiab dej-soluble analytes
  ii. Muaj kev tiv thaiv zoo dua rau biomolecules

4. Adsorption Tswj Tswj Xyuas thiab cov ntaub ntawv

a. Passivation teebmeem
  Kuv. Cov lo lo av hlau render iav hydrophobic, thaiv polar khi
  ii. Ruaj khov tom qab txuas ntxiv nkag rau hauv ACN lossis Meoh

b. Kev ua tau zoo rov qab
  Kuv. Silanized vials tswj hwm ze-100% rov qab rau 1 ppb deexepin dhau sijhawm
  ii. Peg-coated vials ua tiav 97-99% kev rov qab rau Peny Polar β-lactams tshaj 72 h versus 70-80% ntawm tsis siv khob
  III. PFDCS Vials tshaj 90% kev rov qab rau Pahs piv rau cov txiaj ntsig qis dua ntawm cov iav liab qab

c. Tus txheeb ze tshaj tawm qeb duas
  Kuv. Polar Analytes: PeG> Silanized ≈ Pfdcs> Deactivated
  ii. Nonpar Pasistes: PFDCs> Silanized> Deactivated> Peg

5. Thov xaiv thiab kev coj ua zoo tshaj plaws

a. Phim kev kho mob kom ua piv txwv chemistry
  Kuv. Cov pa luam yeeb polar (cov tshuaj, cov protein, carbohydrates): Siv cov lus luv lossis peg
  ii. Tsis yog Organcor (PAHS, Lipophilic toxins): Siv PFDCs Txheej Txheem
  III. Sib xyaw ua piv txwv: Silanization muaj kev ua tau zoo

b. Xav txog cov kuab tshuaj thiab ib puag ncig
  Kuv. Kev ua haujlwm siab ntsoo ua kom muaj kev ywj pheej ua rau PH 1-12 thiab feem ntau cov organics
  ii. Polymer txheej yuav txwv nyob rau hauv cov muaj zog oxidizers lossis cua sov; Xav txog PTFE ntxig lossis polypropylene vials rau huab cua

c. Qauv ntim thiab txhaj tshuaj tiv thaiv
  Kuv. Rau microvolumes (<100 μl) lossis rov ua piv txwv, siv cov txheej txheej ruaj khov
  ii. Saib xyuas cov txheej txheem kev sib raug zoo los ntawm lub kaum sab xis sib tham (> ± 10 ± 10 ± 10 ± 10 ± 10 ± 10 ± 10 ± 10 ± khiav ntawm m \ / z 207, 281)

d. Pob Nyiaj Siv Khoom Siv Nyiaj Txiag
  Kuv. Kev txiav tawm: Tus nqi qis tshaj, haum rau kev qhia lossis cov ntxaij vab tshaus
  ii. Silanized vials: Mid-ntau tus nqi, dav HPLC \ / LC-MS Daim Ntawv Thov
  III. Peg \ / pfdcs Txheej ci: tus nqi tshaj plaws, zoo tagnrho rau kev ntsuas bioanalyses tseem ceeb thiab txoj kab kev xeem ib puag ncig

6. Xaus: Los ntawm lub nkoj dhau los ua kom nquag plias interface

Raws li kev ntsuas kev paub txog PPB \ / PPS PPT theem, coj mus qauv vials ua cov inters uas tsis muaj zog. Kev tsom xam cov kev kho qis qis-adsorption hloov pauv tsis muaj kev tiv thaiv tsis muaj peev xwm them rau hauv cov tshuaj tsis taus. Vial xaiv thiab nplaim kev kho yog qhov tseem ceeb hauv cov qib qis npaum li cas. Los ntawm kev sib txuam txheej thev naus laus zis los ua piv txwv cov vials rau mus rau hauv precision cov cuab yeej, txhim kho kev txhim kho zoo tshaj plaws hauv kev khi.

Cov Ntsiab Lus Tseem Ceeb

1. Rau cov tshuaj ultra-rhiab, siv passivated lossis coated vials

2. Sib phim Polarity: Silanized \ / pg rau hydrophilic, PFDCS rau hydrophobic

3. Saib xyuas cov txheej: Cia cov chaw huv si, taug kev sib cuag cov ces kaum, khiav cov kab, hloov ntawm qhov tsis ua haujlwm

4. Sib npaug tus nqi vs. Cov ntaub ntawv ua tau zoo: Cov tsho tiv no saib tau txo cov reruns thiab cuav negatives