Smagesfọdụ nlele ka enweghị ike ịgbanye ya mgbe ịmechara ya. A chọrọ derivatization na-eme ihe na-enweghị nchịkọta uv ma ọ bụ fluorancence na-achọpụta ndị nchọpụta UV ma ọ bụ Flu fluorance.

Na-eme ka ezi uche dịkwuo mma
Na-eme ka nkewa (mgbanwe dị mma)

Okwu enwere ike ịgụnye mfu, mmeghachi omume, mmeghachi omume na-ezughị ezu, nke nwere ike imetụta ezi ma ọ bụ iwebata njehie.

Ịlele ihe nzacha

LC sample ga-abụ uwe na urughuru; irighiri ihe nwere ike imebi injector ma ọ bụ gbochie kọlụm. Nzọụkwụ gụnyere:

Ikwanye nlele tupu imegbu ma lelee maka irighiri ihe, tachady, ma ọ bụ emulsions
Ọ bụrụ na a na-ahụta irighiri ahụ, kpoo ihe atụ ahụ site na iji akpụkpọ ahụ nwere ike ịnwe

Okwu nwere ike: Ngwunye nhichapu, onye mgbasa ozi na-ebelata iwelata ihe ịta, na ndọtị na-akpata njehie.

Ụzọ mmịpụta

Mwepu kewapụ nyochara site na ntinye aka ma ọ bụ na-ewepu ihe mgbochi (dịka ọmụmaatụ, protein). Ihe ndozi ndi ozo kwesiri ibu ihe ojoo, na-agbanwe, di ala na adighi adi nma, ma igbari salele ahu nke oma.

Ngwọta: dithyl ether: etly acetate, dichloromhane, chloroform, benzene, ma ọ bụ ngwakọta.

Mgbe mmịpụta, enwere ike itinye ya ozugbo ma ọ bụ lekwasịrị anya iji nwekwuo uche nyocha.

Mwepu mmiri nke mmiri

Ihe mejupụtara acidic na salts: wepụ adịghị ọcha na solvac solvent, mezie acidic, mgbe ahụ wepụ ma ọ bụ gbanye; ma ọ bụ kpọnwụọ n'okpuru NT ma gbazee na ihe dị mma tupu ntụtụ.
Ihe mejupụtara ya na nnu: Wepụ adịghị ọcha na solvation solvent, mezie ya na nke nkiti, mgbe ahụ wepụ ma ọ bụ gbanye; ma ọ bụ kpọnwụọ n'okpuru NT ma gbazee na ihe dị mma tupu ntụtụ.
Ihe mgbochi na-anọpụ iche: Wepụta adịghị ọcha na solvation solvent, wee nyochaa ozugbo site na resers na-atụgharị.

Mwepu nke na-eme ka mmiri

Wepụ na ahịhịa ndụ ma ọ bụ gbanye kpọmkwem; ma ọ bụ kpọnwụọ n'okpuru NT ma gbazee na ihe dị mma tupu ntụtụ.

Mgbanwe Fit

Ebe enwere ike imerụ mmadụ na nyocha:

Ụdị mmechu	Nsiputa	Usoro mgbochi
Gburugburu ebe obibi	Na-emebi emebi, uzuzu, aerosols	Debe ụlọ dịpụrụ adịpụ, dị ọcha; Wụnye Ac; na-ejikwa iru mmiri <70%; Gbochie mmiri, corrosion, mkpọtụ
Akpa	Iko, seramic, quartz, ihe rọba plastic	Họrọ eriri dabara adaba na ụdị nlele; ọcha nke ọma
Ndeghata	Ndị na-abụghị Chromatography-klas klas	Jiri chromatography, ịdị ọcha, ma ọ bụ ihe ndị na-agụ akwụkwọ ozi

Nkwadebe ọkọlọtọ

Kwadebe usoro ọkọlọtọ nke ime; Jiri otu batch na ụkpụrụ na ihe nlere iji belata njehie
Ihe ndị mmadụ ga-abụrịrị chromatography-klas na kwụsie ike
Chekwaa ihe ngwọta ọkọlọtọ na brown karama na obere okpomọkụ
Maka vitamin, jiri Volumetric na-acha aja aja ma ọ bụ chebe ya

Nsogbu ndị a na-ahụkarị na nlele protinment na ngwọta

Nsogbu	Mkpatara	Osisa
A na-ejighị ọnụ na mbara igwe na Chromatogram	Ndi mmeru	Sook nzacha na nlele na ntụtụ nwale Gbanwee ụdị nzacha Jiri usoro nhicha ndị ọzọ
Nzaghachi dị ọnụ ala (ọkachasị nlele dị ala)	Mgbasa ozi na elu nza	Gbanwee ụdị nzacha Usoro niile nlele n'okpuru otu ọnọdụ Jiri usoro nhicha ndị ọzọ
Iweghachite	Mịrị amị	Mụbaa oge mbata, jiri ihe mgbatị ọkụ Mepee usoro nhicha
Uwe dị elu, nke na-adịte aka	Nlegharị anya na mmetọ	Meziwanye usoro nhicha
Nkenke ezighi ezi	Iweghachite zuru ezu	Bulie ma ọ bụ dochie derivatization, nkewa, mmịpụta, ma ọ bụ ọnọdụ ndị ọzọ Jiri njikwa akpaghị aka iji meziwanye

Nke gara aga:

Oke dị elu \ / obere okpomọkụ na ngosipụta ọkụ na nkwụsị ọkụ na nkwụsi ike: usoro ọmụmụ

Osote:

Laghachi na ndepụta