Na nkwadebe nlele nke HPLC, ndị na-eme nzacha na-arụ ọrụ dị oke egwu. Na-agwakọ na nnọkọ nwere ike pịa kọlụm, mkpụmkpụ kọlụm, na-egbute ihe mgbaàmà, ma kwenye ịmụpụta ya. Pre-feltration na-ewepu adịghị mma ndị a, na-eme ka ikwugharị na nkwụghachi ụgwọ na nkwụsi ike. Synters na-eme nzacha na-enye ngwọta otu agịga, na-eme ka ihe nlele dị mma dị ọcha tupu ntụtụ ahụ, na-echebe ma kọlụm na ngwa ọrụ

Njirimara isi ihe dị iche iche

PTFE (polytetraFoorooroorooroo) membranes

Pritasilitencobility:Na-enweghị nchegharị ma na-eguzogide ihe fọrọ nke nta ka ọ bụrụ ihe niile soloction, acid, na ntọala, mana hydrophophobic-ekwesighi maka nzacha apịtị apịtị. Ezigbo maka samplents dị elu (e.G., acetonitrile, dmbu), dị elu), na gburugburu ya. Ndị wepụrụ n'efu ọ bụla enweghị wepụta.
Ọnụego agbago:Ihe owuwu na-achọ iji sottent sochiri emechi, na-ebute ọnụego dị ala.
Protein jikọtara: GwDrophobic elu na-amịpụta obere protein protein prodoorption, na-eme ka ọ dị mma maka nyocha nke trace.Jiri ikpe & mgbochi:Kacha mma maka ndị ọrụ ahịhịa dị mma ma ọ bụ nke dị oke egwu (Digest Liquids, extracts recreats). Ọ bụghị maka mmiri na-enweghị paịlị; Zere ihicha akpụkpọ mmiri iji gbochie anya mmiri.

Nylon membranes
Pritasilitencobility:Hydrophilic; dakọtara na mmiri na ọtụtụ ihe mgbaze. Sincents na-emekarị dị ka acetonitrile na DMSo na-akwado nke ọma, ebe ọ na-akwado Methanol na nke dị elu na-adabere. Steal na-agbada na acid \ / ntọala.Ọnụego agbago: Cwets moderate na nzacha ngwa ngwa na flester ngwa ngwa karịa PTFE, mana ọ na-adị nwayọ nwayọ karịa Pvdf.Protein jikọtara:Mgbasa ozi dị elu - ekwesighi maka protein-richasị ihe nlele kachasị. Jiri ikpe & mgbochi: Prosatail maka ihe mgbaze ma ọ bụ ihe nlele dị iche iche ma ọ bụ ihe nlele dị mma (E.G., acetonitrile, methanol). Ragged na autoclavidble, kama zere protein ma ọ bụ sie ike - acid \ / ntụpọ.

PVDF (Polyvinylidlidene fluoride) membranes
Pritasilitencobility:Hydrophilic; Na mmiri, mmanya, na Ketones n'okpuru nnọpụiche acidic \ / dị mkpa. Nnọọ ala UV dị ala na-anabata ndọpụ ndabere.Ọnụego agbago: Wetsdị igwe kwụ ọtọ, na-enye ihe nzacha ngwa ngwa karịa ptfe n'otu paịlịProtein jikọtara:Dị ala nke nwere ihe nlere, protein, na ọdịbendị mgbasa ozi.
Jiri ikpe & mgbochi:Ezigbo maka protein \ / peptoration ma ọ bụ ihe mkpuchi mmanya na-egbu egbu. Na-emetụ aka na ntọala siri ike, nke nwere ike wedara akpụkpọ ahụ.

Pc (polycarbonate) membranes
Pritasilitencobility: Ezigbo, juputara dum kwekọrọ na otutu acids, nnu, mmanụ, na hydrocarbbons. Ọ bụghị na Methanol, Ketones, eyers, ma ọ bụ aromatics.Ọnụego agbago:Dị oke elu nkesa na-enye nnukwu azụ azụ na mmiri ngwa ngwa.Protein jikọtara:Obere iko dị elu na-egbochi mgbasa protein na nnukwu ọtụtụ ụmụ irighiri mmiri.
Jiri ikpe & mgbochi:N'iru ya maka urughuru ọgụgụ, nyocha microscopic, na agụmakwụkwọ chọrọ njigide. Adịghị ejikarị ngwa agha ahịhịa na-eme ka ọ dị mma.

Ntụnyere arụmọrụ arụmọrụ

Enyombone	Mmetụta kemịkalụ	Ọnụego asọmpi	Protein mgbochi	Ngwa & oke
Potfe	Na-eguzogide ihe fọrọ nke nta ka ọ bụrụ ihe mgbaze niile; hydrophobic	Kediala	Dị ala	Firshers hears ofsh; ọ bụghị maka ọdịmma nke mmiri
Nilhon	Hydrophilic; Ezi na acn, dmso; Idogbu na THF, Meh	Imeihenokara	Keelu	Organislọ Ọrụ Ngalaba \ / Buffers; Zere protein & siri ike acids
Puvdf	Hydrophilic; Gburugburu mmiri, mmanya, Ketones	Keelu	Kediala	Ọmụmụ ihe omimi Zere ntọala siri ike
Plus	Ire ụtọ; Good na acid, nnu, mmanụ; Ọ bụghị maka Meh, Ketones	Dị elu	Dị ala	Urughuru \ / microscopy; Ọ bụghị usoro ọmụmụ HPLC

Na-ahọpụta akpụkpọ ahụ aka nri maka nlele gị

Swssh ma ọ bụ nnukwu mmachi + na-achọpụta nyocha: MwerePotfeN'ihi na-egbochi ọgwụ mgbochi kemịkalụ na enweghị oghere.

Ihe nlele ndu (protein) + mmanya \ / MwerePuvdfN'ihi na obere protein protein na ndabara ga-agbagha.
Ihe mgbazinye ihe omumu ihe omumu:MwereNilhonMaka nkwekọ ya niile na ịdịte aka - zere protein ma ọ bụ PH dị oke oke.
Pructughtslọ urughuru \ / microscopy: MwerePlusmaka zuru oke na ntụpọ mgbasa ozi.

Mara:Na-ewere ihe nlere anya mgbe niile, ọnụ ahịa, na akụrụngwa mgbe ị na-eme nhọrọ ikpeazụ gị.

Aijen na-enye Anijers na PTFE na PTFE, Nylon, Pvdf, na PC membranes iji mezuo mkpa nzacha ngwa ngwa HPLC gị niile. Kpọtụrụ anyị maka nlele ma ọ bụ nyocha ọrụ, ka anyị nyere gị aka ịhọrọ usoro kachasị mma.

Nke gara aga:

vials dị ala, ọgwụgwọ na-agbanwe agbanwe, vials elized, vials dị elu, pfdcs mkpuchi

Osote:

Oke dị elu \ / obere okpomọkụ na ngosipụta ọkụ na nkwụsị ọkụ na nkwụsi ike: usoro ọmụmụ