Ndi zinthu zosasunthika zomwe sizikusanthula ndi GC-MS?

Ma chromatography-Mass Spectry (GC-MS) ndi njira yamphamvu yowunikira yomwe imagwiritsidwa ntchito posanthula kwambiri kusanthula votile ndi semivolatile. Komabe, zitha kugwiritsidwanso ntchito kusanthula mitundu yosagwirizana ndi njira zosiyanasiyana, kuphatikizapo kutaya mtima. Nkhaniyi ikuwunikira mitundu ya mankhwala osagwirizana ndi GC-MS, kufunikira kwake, komanso njira zomwe zimagwiritsidwa ntchito kuzizindikira.

Mukufuna kudziwa zambiri za kusiyana pakati pa LC-MS ndi GC-MS, chonde onani nkhaniyi:Kodi pali kusiyana kotani pakati pa LC-MS ndi GC-MS?

Kodi osagwirizana ndi chiyani?

Magawo omwe sakhalapo ndi zinthu zomwe sizimasintha mosavuta kutentha. Amakhala olemera kwambiri komanso ochulukirapo, kuwapangitsa kukhala oyenera pang'ono kuwunika mwachindunji ndi GC-Ms Popanda kusintha. Zitsanzo zodziwika bwino zimaphatikizapo:

Ma polima ndi owonjezera: zinthu zomwe zimagwiritsidwa ntchito mu mapulaji ndi zida zopangira.

Biosolecles: monga amino acid, mapuloteni, ndi lipids ena.

Mankhwala ogulitsa: Zosakaniza zamankhwala (apis) ndi metabolites yawo.

Zoyipitsa zachilengedwe: zolimbitsa thupi zolimba (pops) ndi zitsulo zolemera.

Njira Zosanza

Kusanthula zinthu zomwe sizikugwirizana ndi GC-MS, zosemphana nthawi zambiri zimafunikira. Izi zimakhudza kusintha mwanjira yopuma kuti iwonjezere ma volatility kapena kukhazikika. Njira zodziwika bwino za Derivatation zimaphatikizapo:

Silnurization: Kusintha maatomu a hydrogen mu gulu logwira ntchito ndi gulu la silicon (E.g., Trimethylyl). Njirayi imathandizanso kumwa mowa, Amines, ndi Carboxylic acid.

Macylation: Njira iyi imayambitsa magulu a acyl kuti ipititse patsogolo kusukololo ndipo nthawi zambiri amagwiritsidwa ntchito mafuta acids ndi amino acid.

Methylation: Njira iyi imawonjezera magulu a methyl kuti aphatikize kuwononga mavu osazindikira komanso osazindikira.

Njira zosiyidwa izi zimatha kusintha zinthu zosasunthika kukhala mawonekedwe omwe amatha kusanthula bwino ndi GC-MS.

Kuti mumve zambiri pa Autosambir Mbale za Chromatography, fotokozerani nkhaniyi:2 ml Autosambir Mbale za Chrometography

Kodi ndi zinthu zosafunikira zomwe sizingagwiritsidwe ntchito?

1. Zangozi Zachilengedwe

GC-MS imagwiritsidwa ntchito kwambiri kusanthula zinthu zosasunthika zowopsa zomwe zimalembedwa ndi mabungwe azachilengedwe. Mwachitsanzo, bungwe loteteza chilengedwe (EPA) lasankha njira zopenda zoipitsa monga:

Mapulogalamu a Polychlornated (PCBS): mankhwala odziwika bwino chifukwa cha kulimbikira.

Mankhwala ophera tizilombo: zotsalira zochokera ku ulimiro zomwe zimayipitsa nthaka ndi madzi.

Makhalidwe ozindikira izi ndi pakati pa 1 ndi 28 ppb, akuwonetsa chidwi chachikulu cha gc-m mas akaphatikizidwa ndi maluso oyenera monga gawo lolimba monga gawo lolimba lotere.

2. Kusanthula kwa Chitetezo cha Zakudya

M'dera la chitetezo cha chakudya, GC-ms limagwiritsidwa ntchito kuzindikira zodetsa zosasunthika zomwe zingasamuke pazida zopangira chakudya. Izi zimaphatikizapo:

Mapulasitiki: Mankhwala owonjezeredwa kwa mapulaneti kuti azitha kusinthasinthasintha; Zitsanzo zimaphatikizapo Phtates.

Mwachitsanzo: Mwachitsanzo, antioxidants kapena oteteza zinthu zomwe zingalitse chakudya.

Kutha kusanthula zinthu izi ndikofunikira kuti muwonetsetse chitetezo cha ogula komanso kutsatira malamulo oyang'anira.

3. Mankhwala opanga mankhwala

Kusanthula kwa mankhwala nthawi zambiri kumafuna kuzindikiritsa kwa zinthu zosasunthika ndi ma metabolites. Zitsanzo Zimaphatikizapo:

Yogwira mankhwala osokoneza bongo (API): Zoyenera zoyambirira zomwe zimapangitsa kuchiritsa.

Metabolites: Zopangidwa zopangidwa mu kagayidwe ka mankhwala mkati mwa njira yachilengedwe.

GC-MS imalola kusanthula mwatsatanetsatane kwa mankhwala awa, pothandizira mu maphunziro a pharmacokinetic ndi mankhwala omwe akupanga mankhwala.

4. Zitsanzo zazomwe

Mu metabolomics, GC-ms imagwiritsidwa ntchito posanthula metabolile metabolites m'malo ovuta azomwe ali ndi mkodzo kapena magazi. Mafuta omwe anthu ambiri amasanthula ndi:

Amacino acid: kumanga mapuloteni a mapuloteni, omwe amatha kuwonetsa mawonekedwe azakudya kapena kusokonezeka kagayidwe kachakudya.

Macitic acids: metabolites omwe amakhudzidwa ndi njira zosiyanasiyana zam'madzi.

Kugwiritsa ntchito ndikofunikira kuti timvetsetse zikwangwani za metabolic munthawi yathanzi komanso matenda.

Njira za GC-MS

Kukonzekera zitsanzo

Mukamasanthula zinthu zosasunthika pogwiritsa ntchito GC-ms, kukonzekera kwakukulu ndikofunikira. Njira zingaphatikizepo:

Madzimadzi amadzimadzi (le): amalekanitsa mosasintha kuchokera matrict am'magulu am'madzi.

Gawo lolimba m'zigawo (SPE): Pursents amaganiza zosinthana ndi zosanthu zambiri asanasanthule.

Chipangizo

Kukhazikitsa kwa GC-MS kumaphatikizapo:

Chromatograph: imalekanitsa zosasunthika zochokera pogawana nawo pakati pa statery ndi ma gasi yamagesi.

Misa Spectrometer: Zizindikiritsa mankhwala kutengera kuchuluka kwa magalimoto akuluakulu (m \ / z), popereka chidziwitso.

Kusanthula kwa data

Mass spectrum akapezeka, kusanthula kwa deta kumaphatikizapo kufananiza misa yodziwika ku laibulale kapena database kuzindikiritsa. Madaki otsogola akhama amathandizira kuyerekezera uku, kenako kulimbikitsa chizindikiritso.

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Mapeto

Ma chromatography - misa spectometry imakhalabeukadaulo wofunikira pamankhwala owunikira chifukwa cha mankhwala omwe sayansi, chitetezo cha chakudya, komanso mermabolimics, komanso merbolimics. Kusanthula mwachindunji kwa mankhwalawa ndikovuta chifukwa cha kuchuluka kwa zinthu zomwe zimachitika, njira zosiyidwa zatha kwambiri ndikugwiritsa ntchito mapulogalamu a GC-MS. Monga njira zowunikira mosakhalitsa zikupitiliza kusinthika, GC-MS ikuyenera kuchita gawo lofunikira kwambiri pakuwonetsetsa chitetezo ndikutsatira mafakitale akupita patsogolo pa kafukufuku wa sayansi.