Kodi pali kusiyana kotani pakati pa gc-ms ndi gc-ms \ / ms?

Gasi chromatography-misa sportmetry (GC-MS) ndi mpweya chrometography-tandem spectometry (GC-MS Sportmicals (GC-/ A MS) ali ndi luso lothana ndi zamankhwala osiyanasiyana, sayansi yachilengedwe, komanso chitetezo cha chakudya. Ngakhale njira zonse ziwiri zimagwiritsa ntchito chromatography (GC) yolekanitsa ndi misa spectrometry (ms) kuti muzindikire, zimasiyana kwambiri pamachitidwe awo, kuthekera, ndi ntchito. Nkhaniyi ikuwunikira mwatsatanetsatane.

Kodi GC-MS ndi chiyani?

Kukonzekera zitsanzo

Gawo lolimba m'zigawo (Spe) kapena madzi-madzi kusefukira (LLE) nthawi zambiri imagwiritsidwa ntchito kuchotsa zosokoneza matrix ndikuwonjezera chidwi.

Derivatation (E.g., methylation, trimethylkylky) imatha kukonza zotupa za polar kapena mafuta a Labile.

Momwe Zimagwirira Ntchito

GC-MS kuphatikiza gasi chromatography ndi spectrometry yowunikira zosakaniza zovuta. Panthawi imeneyi, chitsanzo chimasinthidwa ndikutumizidwa kudzera pa chromatographic column pogwiritsa ntchito gasi wa bart ngati gawo lam'manja. Pamene mankhwalawa amalekanitsidwa kutengera kusasinthika kwawo ndi gawo la masirisi, amayambitsidwa kukhala wowoneka bwino.

Zigawo za GC-MS

Chromatography: imalekanitsa anthu osakanizika mosiyanasiyana malinga ndi malo awo otentha komanso omwe amawagwiritsa ntchito.

Misa Spectrometer: Amazindikira ndikuzindikira mankhwala olekanitsidwa pofuna kuchuluka kwa kuchuluka kwa maumboni aikulu (m \ / z). Zotsatira zambiri zimapereka chidziwitso chokhudza kuchuluka kwa zochulukitsa komanso kapangidwe kake.

Magwero a Ionization

Njira zofewa zamankhwala (E.g., APCI, dart) amachepetsa kugawanika ndikuwonjezera ma ineracular.
Makina a GC-MS tsopano amagwiritsidwa ntchito pa tsamba loopsa kupezeka ndi kuwunika zachilengedwe.

Ntchito za GC-MS

GC-MS ili ndi ntchito zosiyanasiyana, kuphatikiza:

Kusanthula Komwe: Kuzindikira mankhwala, poizoni, ndi zinthu zina zomwe zitsanzo za zitsanzozo.

Kuyang'anira zachilengedwe: kusanthula kodetsa nkhawa mumlengalenga, madzi, ndi dothi.

Mankhwala ogulitsa: Kuwongolera kwapadera komanso njira yopangira mankhwala.

Chitetezo Chakudya: Kufufuza zodetsa ndi kutsimikizira za chakudya.

Makampani opanga ma petroleum: Kusanthula kwa kapangidwe kake kosweka ndi mafuta osungunuka, kuchuluka kwa zigawo za gasi.
Metabolomics: Kupenda ndi kuchuluka kwa ma metabolites ang'onoang'ono, kugwiritsa ntchito ziwerengero zambiri kuti mupeze biomari.

Kodi GC-MS \ / ms?

Momwe Zimagwirira Ntchito

GC-MS \ / MS imawonjezera kuthekera kwachikhalidwe cha GC-MS mwa kuphatikiza tandem misa spectrometry. Izi zikutanthauza kuti pambuyo pa kusanthula koyambirira kwa Spectrometry (ms), ma ions osankhidwa amaphatikizidwanso mu gawo lachiwiri la mawonekedwe a misa spectrometry (MS / MS). Njira ziwirizi zitha kufotokozera mwatsatanetsatane zokhudzana ndi kusanthula.

Zigawo za GC-MS \ / ms

Q1 yoyamba (Q1): Ntchito ngati mawonekedwe a misa wamba, kusankha ma ions potengera kuchuluka kwawo kwa m \ / z.

Cell Cell: Ma ions osankhidwa amaphatikizidwa ndi kupatsidwana ndi kugundana kwa kugundana (CID), kupanga ma iyo.

Q2ERUESTROROBEL (Q2): Chidutswa cha ma nthito a ma qamegragy chimasanthuridwa kuti chikuperekedwe kowonjezera komanso chidwi.

Ion Cuck \ / Kachitatu-gawo la GC-MS \ / MS Syspys amatanthauza msampha wa ion kapena gawo lachitatu la magawo ankhondo akuzama.

Ntchito za GC-ms \ / ms

Chidwi chowonjezera komanso chizindikiritso cha GC-MS \ / ms zimapangitsa kuti ikhale yoyenera:

Kuchuluka kwa chandamale

Kusankhidwa kosakaniza: Kuzindikiritsa mankhwala osokoneza bongo omwe amasinthana nawo.

Kuyesa kwachilengedwe: Kuzindikira kuti ali ndi vuto lomwe limafunikira chidwi chachikulu.

Kuwerenga Kwambiri Kwambiri: Kugwiritsa ntchito njira za GC mwachangu ndi kuwunikira zingapo (mrm) kuti mupeze mankhwala ambiri ophera tizilombo nthawi imodzi.
Zakudya Zakudya ndi Kusanja: Kuzindikira zachiwerewere ndi zoyambira zoyambira za Geographic zodziwika bwino za ma ion.

Kusiyanitsa kwakukulu pakati pa gc-ms ndi gc-ms \ / ms

1. Malingaliro ndi mawonekedwe

GC-MS: Amapereka chizindikiritso choyambira kutengera nthawi yosungira komanso kuchuluka misa, koma mwina kuvutika ndi kusakaniza kovuta komwe kuli kolunjika.

GC-MS \ / MS: chidwi chachikulu chifukwa chokhoza kupenda ma matragragration, kulola kuzindikira koyenera ngakhale manyowa ovuta. Izi zimapangitsa kuti zizikhala zothandiza kwambiri pakuwona kuchuluka kochepa.

2. Malire

GC-MS: Malire ozindikira nthawi zambiri amakhala okwera poyerekeza ndi gc-ms \ / ms. Itha kuzindikira mankhwala, koma mwina sangakhale nawo molondola pazotsika kwambiri.

GC-MS \ / MS: Kusankha Kusankha Kwambiri Kuyang'anira (marrm) kapena kuwunika kosankhidwa (srrm)

3. Kuchita zinthu

GC-MS: Amatulutsa misa imodzi kwa gawo lililonse la anthu omwe apezeka, lomwe limatha kugwiritsa ntchito zambiri koma mwina sizingafotokozere mwatsatanetsatane.

GC-MS \ / MS: Amapanga ziwonetsero zingapo za kafukufuku aliyense potengera mawonekedwe, ndikuwonetsa kuzindikira kwakukulu kwa mawonekedwe osokoneza bongo ndikuthandizira kuwunika kwathunthu.

4. Kuchita masewera olimbitsa thupi

GC-MS: Nthawi zambiri zosavuta kugwira ntchito ndipo zimaphatikizapo zochepa; yoyenera kusanthula kwa chizolowezi chofunsira kwambiri.

GC-MS \ / MS: zovuta kwambiri chifukwa cha kuwonjezera kwa zigawo monga ma cell a Collions ndi zigawo zingapo; pamafunika maphunziro apadera kuti atanthauzidwe ndi kutanthauzira kwa deta.

5. Zovuta

GC-MS: Nthawi zambiri okwera mtengo mu ndalama zoyambirira komanso ndalama zogwirira ntchito; Oyenera ma labootories okhala ndi bajeti ochepa.

GC-MS \ / MS: ili ndi mtengo woyamba wa ukadaulo wapamwamba ndikuwonjezera kukonzanso; Komabe, limapereka mwayi wowunikira kwambiri womwe ungapangitse kuti azigulitsa mapulogalamu apadera.

FAQ

Q: Kodi pali kusiyana kotani pakati pa gc-ms ndi gc-ms \ / ms?
A: GC-MS \ / MS imalimbikitsa chidwi ndi kufikiridwa powonjezera gawo lachiwiri la spectrametry, kulola kuzindikiritsa koyenera kwa mankhwala, makamaka osakaniza osokoneza bongo.

Q: Ndidzasankha liti GC-MS pa GC-MS \ / ms?
Yankho: GC-MS ndiyoyenera kusanthula kwa zinthu zosasunthika komwe chidwi chachikulu sichotsutsa. GC-MS \ / MS imakonda kuzindikira mateli otsika mu matricts.

Q: Kodi GC-MS ndi GC-MS \ / MS yoyenera pazinthu zosasunthika?
A: Njira zonsezi zimapangidwa makamaka chifukwa cha kusakhazikika komanso kokhazikika. Mankhwala osasunthika angafunikire kuchepa kapena njira zina monga LC-MS.

Q: Kodi ndalama zimafananitsa pakati pa gc-ms ndi gc-ms \ / ms?
A: Makina a GC-MS nthawi zambiri amakhala otsika mtengo ndipo ali ndi ndalama zochepa zogwirira ntchito. GC-MS \ / Makina a MS amaphatikiza ndalama zowonjezera ndi zolipiritsa chifukwa cha luso lawo lakutsogolo.

Q: Ndi mitundu yanji ya mankhwala omwe angachitike?
A: GC-MS ndiyoyenera kusinthika kapena kosasunthika monga Pahs, mankhwala ophera tizilombo, VCS, ndi mankhwala. Kusilira kumakulitsa kukula kwake ku polars ndi ma amino acid ndi shuga.

Q: Kodi zitsanzo ziyenera kukonzekera bwanji GC-MS?
Yankho: Kukonzekera zitsanzo nthawi zambiri kumafuna kusefa, dpe kapena khwangwala kuchotsa matriki. Derivatation (E.g., methylation, sychilation) ndizofunikira pa polar kapena mafuta a Labile. Pamitundu yovuta (E.g., Magazi, dothi), Kuyeretsa Kwambiri Silica gel a Gel Column Chromagraphy ikulimbikitsidwa.

Q: Kodi malire a GC-MS ndi chiyani?
A: malire a GC-MS nthawi zambiri amakhala mu mzere wa NG-PG, kutengera ndi zida ndi zitsanzo pokonzekera. Kusanthula kwa mankhwala osokoneza bongo, kumatha kufikira 1-10pg.

Q: Kodi ndi kuchuluka kwa kuchuluka kwa ma gc-m sikumapenda?
Yankho: Chifukwa sayansi iyenera kupatsana, GC-MS nthawi zambiri imasanthula mamolekyulu mpaka 800Da. Ndi mizati yamagetsi yamagetsi yotentha kwambiri komanso kunyoza, izi zitha kuwonjezera ~ 1000DA. Kwa mamolekyulu akulu, LC-MS ikulimbikitsidwa.

Q: Kodi ndimasankha bwanji pakati pa GC-MS ndi GC-MS \ / ms?
Yankho: Pakuwerengera kapena matriction matriction (E.g., zitsanzo zachilengedwe kapena zachilengedwe), GC-MS \ / MS ikulimbikitsidwa kuti ikhale yolondola.

Mukufuna kudziwa zambiri za kusiyana pakati pa LC-MS ndi GC-MS, chonde onani nkhaniyi:Kodi pali kusiyana kotani pakati pa LC-MS ndi GC-MS?

Zowoneka bwino

Tebulo ili limathandizira kumvetsetsa bwino kusiyana pakati pa njira ziwiri.

Mwachidule, GC-MS ndi GC-MS \ / MS ndi njira zamphamvu zowunikira zomwe zimatenga gawo lofunikira m'magawo osiyanasiyana asayansi. Pomwe GC-MS ndiyoyenera kusanthula kwazinthu zosungunulira, gc-ms \ / ms imapereka chidwi, mwachindunji, komanso chidziwitso kudzera mwa tandem misa. Kusankha pakati pa njira ziwirizi kumatengera zofunikira mwatsatanetsatane za kuwunika komwe kumachitidwa, kuphatikizapo chidwi, kuphatikizapo matrix kutrix, malingaliro a ndalama, ndi ntchito ya labotale. Kumvetsetsa izi kumapangitsa ofufuza kuti asankhe njira yomwe imayenereradi zosowa zawo zowunikira, kuonetsetsa kuti zomwe apeza ndizolondola.