Chromatography ndi njira yofunika kwambiri yopezeredwa yomwe imagwiritsidwa ntchito m'magulu osiyanasiyana sayansi monga chemistry, biocheminry ndi njira yamankhwala, biochemistry ndi njira yasayansi, komanso sayansi yachilengedwe. Imakhala ndi gawo lofunikira pofuna kupatutsa, kuzindikira, komanso kuchuluka kwa zigawo zingapo. Chinthu chimodzi chomwe chimakhudza zotsatira zake zowunikira chikhoza kukhala voliyumu ya vial kale kugwiritsa ntchito zitsanzo; M'nkhaniyi, tifufuza gawo lotsutsali lofunikira kwambiri.

Kumvetsetsa chromatography

Chromatography ndi njira ya labotale yolekanitsa ndi kusanthula zosakaniza. Imagwiritsa ntchito magawo awiri, gawo lam'manja (lomwe lingakhale madzi kapena mpweya) ndi gawo lokhazikika (nthawi zambiri limakhazikika kapena madzi), kuti mufufuze zosakanikirana zilizonse. Akasakanizidwa m'dongosolo, magawo osiyanasiyana amakhudzana ndi gawo lokhazikika ndi gawo lokhazikika ndikuyenda mosiyanasiyana ndikuyenda mosiyanasiyana.

Kusanthula Kwambiri ndi Kusanthula kwa chromatography

Mbale zomwe zimagwiritsidwa ntchito mu chromatographic kusanthula kumatha kukhala ndi chisonkhezero chachikulu pazotsatira. Gawo limodzi lofunikira kuti muiwale poyang'ana zitsanzo za zitsanzovoliyumu ya vial. Umu ndi momwe zingakhudzire kusanthula kwachitsanzo:

Direshi:Kuwonjezeka kwa Vali kuli kumayambitsa zitsanzo zofananira kukhazikika pang'onopang'ono, zomwe zimapangitsa kuti azithana ndi zitsanzo ngati kuchuluka kwa vial. Ngati magawo ogwirizana ndi ogwirizana ndi ofunikira kuti muwunikidwe, kusanthula kumeneku sikungapange zotsatira zotsatila komanso kusintha.

Maonera Owona:Kusanthula kwa chromatograph kumafunanso malire enieni mukamachita ndi kusanthula kwa trace kapena zitsanzo zotsika kwambiri, monga tracer yestres kapena zitsanzo zotsika kwambiri. Voliozere kakang'ono kwambiri kumatha kuthandiza kukwaniritsa malire ocheperako monga kuchuluka kwa zitsanzo zokulirapo zikakhala m'malo ang'onoang'ono.

Chidwi:Njira za chromatographic nthawi zambiri zimawerengera chidwi chawo ndikusanthula; Magetsi ang'onoang'ono amatha kuwonjezera chidwi ichi, ndikupangitsa kukhala kosavuta kudziwa komanso kuchuluka kwambiri.

Zosungunulira:Kugwiritsa ntchito mawu ang'onoang'ono ang'ono kumafunikira kuwunika pang'ono pofufuza, zomwe zitha kusunga ndalama ndikukhala bwino chilengedwe.

Zotsatira za Matrix:Pa zitsanzo zovuta, pogwiritsa ntchito mitundu yaying'ono ya vial imatha kuthandiza matrix matrix. Zotsatira za matrix zimachitika pamene mankhwala ena mu sampu ya matrix amasokoneza kusanthula kosakonzekera; Mwakuchepetsa mphamvu iyi imachepetsedwa pazomwe zimachitika.

Mukufunitsitsa kupeza mayankho okwanira okhudza HPLC Vials? Lowani munkhaniyi kuti mudziwe bwino:50 mafunso ambiri omwe amafunsidwa kawiri pa HPLC Mbale

Zotsatira za Peak mawonekedwe ndi lingaliro

Kuyanjana pakati pa zopereka ndi mafoni kusewera gawo lofunikira posankha mawonekedwe a Peak. Mukamagwiritsa ntchito zosungunulira ndi mphamvu yayikulu kuposa gawo lam'manja, nsonga zimatha kuwoneka zowonjezereka chifukwa cha zomwe zimachitika mwachangu kwambiri kuposa osankhidwa. Izi zitha kuipira kwambiri voliyumu, zomwe zimapangitsa kuti zisatheke bwino komanso zowoneka bwino.

Komanso, pogwiritsa ntchito zitsanzo zokhala ndi mphamvu zotsika zitha kusintha mawonekedwe a peak polemetsa pakati pa mzati, zomwe zimayambitsa kupatukana bwino ndi osankha. Izi zikugogomezera kufunikira kopanda phindu komanso chifukwa chosankha zosungunulira zomwe zimagwiritsidwa ntchito ndi izi.

Kulingalira

Yaying'onovoliyumu ya vialzitha kupereka chidwi chachikulu ndikusautsika; Komabe, malingaliro othandiza ayenera kuwerengedwanso. Vesi la zitsanzo kuyenera kukwaniritsa zofunikira zowunikira; Makina ang'onoang'ono sangakhale oyenera nthawi zonse.

Nthawi zina ofufuza amasankha kusanthula zitsanzo zawo asanasanthula, kuwathandiza kugwiritsa ntchito mavolinamiyu ocheperako osawononga zitsanzo za kukhulupirika.

Mapeto

Voliyumu ya VIA ndikuganizira kwambiri kusanthula kwa chromatography ndipo iyenera kusankhidwa mosamala kuti mukwaniritse zotsatira zolondola komanso zodalirika. Ofufuzawo ndi ofufuza ayenera kusankha mosamala voliyumu yabwino ya vial yomwe ikukwaniritsa zosowa zawo - zomwe zimapangitsa kuti zikhale zowoneka bwino, matrix zotsatira, ndi zinthu zopindulitsa kwambiri.

Mukuyang'ana kuti mufufuze? Lowani munkhaniyi pamapulogalamu 15 a Mbale Za Chromatograph:Mapulogalamu 15 a Chromatography Vials m'minda yosiyanasiyana

M'mbuyomu:

Mapulogalamu a Mzere wa chromatograph m'matumba ndi kusanthula kwa mpweya

Ena:

Kodi ndingasankhe bwanji kuyika kumanja kwa HPLC yanga?