Gasi chromatography (GC)Njira yofunika yowunikira yomwe imagwiritsidwa ntchito kupatula popanga mankhwala osakaniza. Chimodzi mwazomwe mumayeza mu GC ndi gawo la nsonga, zomwe zimakhudzana ndi kuchuluka kwa kusanthula. Kuzindikira zinthu zomwe zimakhudza dera la Pek ndikofunikira kuti zitheke komanso zotsatira zobereka. Nkhaniyi imayang'ana zinthu zosiyanasiyana zomwe zimakhudza malo a GC Peak, kuphatikiza njira ya jakisoni, zojambula, zodzitchinjiriza, ndi zikhalidwe.

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1. Njira Yopanga jakisoni

Njira ya jakisoni ya zitsanzo imagwira gawo lofunikira posankha dera la Peak:

Voliyumu ya jakisoni: kuchuluka kwa zitsanzo zomwe zakanidwa ziyenera kukhala zolondola. Kuchulukitsa mzati kungayambitse nsonga zosokoneza monga zopepuka kapena zomangirira, zomwe zingakhudze malo oyenerera. Mosiyana, kuchuluka kwa jakisoni wokwanira kumatha kubweretsa mphamvu yotsika komanso malo ochepetsedwa.

Kuthamanga kwa jekeseni: Kuthamanga komwe sayansi imalowetsedwa imathanso kukhudza mawonekedwe a Peak ndi nsonga. Ma jakisoni achangu amatha kuyambitsa mavuto, zomwe zimapangitsa kusakanikirana kosakanikirana ndi gulu lotopetsa, pamapeto pake zimachepetsa dera.

Makina a jakisoni: jakisoni osasiyana nthawi zambiri amagwiritsidwa ntchito pofufuza mwachidziwitso chifukwa amalola kusanthula kwakukulu kuti alowe mzere, zomwe zingakuwonjezere bwino. Mosiyana ndi zimenezo, kudula majekisiwo kuchepetsa zitsanzozo, zomwe zimapangitsa madera ang'onoang'ono azitsanzo zambiri.

2. Mikhalidwe yazikhalidwe

Makhalidwe a mzati ali ndi vuto lalikulu pa dera la Peak:

Mtundu wa Column ndi kukula: Kusankha kwa mzere (E.g., Capillary VS. Yonyamula) imakhudza kutanthauzira ndi kuchita bwino. Mitundu ya capillary nthawi zambiri imakhala ndi mainchesi ochepa komanso malo akuluakulu, zomwe zimapangitsa kuti kupatulize bwino komanso kupindika kwamphamvu.

Kutentha kwamulu: Kuwongolera kutentha ndikofunikira ku GC. Kutentha kwambiri kumatha kupititsa patsogolo kusalation ndikusintha, koma kumathanso kumapangitsa nsonga zazingwe ngati sikuyendetsedwa bwino. Mofananamo, kutentha kochepa kumatha kutengera nthawi yoyaka kwambiri ndikuwonjezera madera ochulukirapo chifukwa chopatukana.

Kuyenda kwamagesi otsika: kuchuluka kwa mpweya wonyamula kumakhudza momwe kafukufuku amasunthira mwachangu kudutsa mzere. Kuchuluka kwa mtengo wowoneka bwino kumayambitsa nsonga zakuthwa; Kuchuluka kwambiri koyenda kumachepetsa kulumikizana pakati pa kafukufukuyu komanso gawo lakale, chifukwa cha madera ang'onoang'ono.

Kodi mukudziwa kusiyana pakati pa VPLC Vials ndi Mbale za GC? Onani nkhaniyi:Kodi pali kusiyana kotani pakati pa mbale ya HPLC ndi Mbale za GC?

3.. Zolemba

Magwiridwe antchito amakhudza mwachindunji ndi kulondola kwa mlingo wa Peak:

Kuzindikira kwa zowona: Zowunikira zosiyanasiyana zimakhala ndi zodetsa zosiyanasiyana kusanthula. Mwachitsanzo, chojambula chamoto chamoto (chod) ndi chovuta kwambiri, koma mwina sichingayankhe chimodzimodzi ku mankhwala onse, zomwe zikukhudza dera loyezedwa.

Nthawi Yoyankha: Nthawi yoyankha yojambulidwa imakhudza momwe zimagwiritsidwira ntchito mwachangu pakusintha. Nthawi zonse kuyankha modekha kumapangitsa chidwi chowonjezera, chomwe chimachepetsa malo owerengedwa.

Kukhazikika kutentha: zojambula zambiri zimakonda kusinthasintha kutentha. Kuonetsetsa kuti kutentha kokhazikika kumathandizanso kuyankha mosasinthasintha komanso kukhazikika kolondola kwa malo a Peak.

4. Mikhalidwe Yachitsanzo

Mtundu wa temple wowunikira umachitanso ntchito yofunika:

Kuzindikira: Kusankhidwa kwa chiwerewere kumakhudza mwachindunji malowo; Kukula kwambiri, kwakukulu pachimake. Komabe, ngati kupanikizana kumapitilira mzere wa chofufuzira, kukwezedwa ndi miyezo yolondola kumabweretsa.

Zotsatira za matrix: kupezeka kwa mankhwala ena mu matrix zovuta kumatha kusokoneza kupezeka kwa kafukufuku. Zigawo za matrix zitha kuyamwa kapena kuzichita ndi chifufuzi, zomwe zimapangitsa kuti nthawi yochepetsedwa kapena nthawi yosintha.

Kutulutsa kwa ma volatili: Kusanthula kwa kafukufuku kumakhudza momwe amachitira ndi ma cerototography. Mankhwala osinthika ambiri afungusi ndipo amatha kupanga nsonga zakuthwa kuposa mitundu yocheperako kuposa mitundu yocheperako, yomwe imatha kuchititsa kuti nthawi yothetsera malo okhala ndi malo osungidwa.

5. Magwiridwe antchito

Magwiridwe antchito onse ndi ofunikira pakupanga kolondola kwa GC:

Phokoso la Baseline: phokoso lalitali limatha kubisa nsonga zazing'ono kapena kuchepetsa kukula kwake, kukhudza kulondola kophatikiza komanso kutsogolera ku zolakwika kumapiri.

Column Columncancy: Kuchepetsa Column Columncancation chifukwa chokalamba kapena kuipitsidwa kumatha kudzetsa m'matumbo a zingwe ndi madera ang'onoang'ono. Kusamalira nthawi zonse ndikuwunika kwa magwiridwe antchito ndi ochititsa chidwi kuti mukwaniritse zotsatira zosasinthasintha.

Kuphatikiza magawo: Pulogalamu yomwe imagwiritsidwa ntchito kuphatikiza nsonga imayenera kukonzedwa molondola. Zosintha zosayenera zimatha kuyambitsa kuwerengera kolakwika chifukwa cha kukhazikitsidwa kolakwika kapena kuphatikizika kophatikiza.

Mukufuna kudziwa zambiri za kusiyana pakati pa LC-MS ndi GC-MS, chonde onani nkhaniyi: Kodi pali kusiyana kotani pakati pa LC-MS ndi GC-MS?

Mapeto

Madera a GC Peak amakhudzidwa ndi zinthu zosiyanasiyana, kuphatikizapo ma jakisoni a jakisoni ndi zojambula, zodzitchinjiriza, ndi zochitika. Kuonetsetsa deta yapamwamba kwambiri, ofufuza amawongolera voliyumu mosamala, kukweza mzere monga kutentha ndi kuwotcha, ndikusungabe zowunikira kudzera mu utsogoleri wokha. Ayeneranso kuganizira za matrix zotsatira pokonzekera zitsanzo. Mwa kutchula nkhani izi, ma labotorees amatha kukonza molondola komanso kudalirika kwa chromatographic kuwunika, pamapeto pake kupeza zotsatira zodalirika kwambiri.

M'mbuyomu:

Kodi vial vial ndi chofunikira kwambiri mu chromatography ndi chiani?

Ena:

Ma Chuma cha Chuma cha COD (HG-Free): Zomwe muyenera kudziwa