Chromatographragraphramita, njira yofunikira mu sayansi ya mankhwala ndi zowunikira, imagwiritsidwa ntchito kwambiri polekanitsa komanso kusanthula zosakaniza zovuta. Imapeza mapulogalamu m'magulu a mafakitale, kuchokera ku mankhwala owunikira zachilengedwe. Mbale ndi Maulendo Awiri Amasewera Panjira Yofunika mu Njirayi - Zingwe zagalasi zakhala zotchuka chifukwa cha zabwino zake; Tiona chifukwa chake kutchuka kwawo kumapitilira m'nkhaniyi.

Kumvetsetsa chromatography

Musanatsuke mwakuya mu magalasi ophatikizira, tiyeni tiwone mwachidule zoyambira za chromatography. Chromatography ndi njira yolekanitsa zosasinthika m'magulu awo pogwiritsa ntchito zigawo zawo zokhala ndi magawo awiri - nthawi zambiri zimakhala zamadzimadzi kapena madzi awiri - nthawi zambiri. Zigawo zazikuluzikulu za chromatographic dongosolo lililonse ndi mzatiyo, makanda, ndi kulowetsa.

Gawo la magalasi ophatikizira

Magalasi Okhazikikandi machubu ang'onoang'ono agalasi opangidwa makamaka kuti agwirizane mkati mwa makosi omwe amagwiritsidwa ntchito chromatotography. Ngakhale pali mitundu yosiyanasiyana ndi mawonekedwe a zigawo zolumikizira, mawonekedwe awo okhazikika amakhalabe imodzi mwazoyesedwa kwambiri chifukwa cha zabwino zake zambiri.

Ubwino wa Magalasi Okhazikika mu chromatography

Magalasi a Magalasi Amapereka Kuwongolera Konzani:

Chimodzi mwazabwino kwambiri zamagalasi zigawo zamagalasi ndi kuthekera kwawo kowongolera kusinthasintha. Mapangidwe awo omwe amaphatikizidwa pansi omwe amachepetsa malo omwe ali ndi mpweya - izi ndi zopindulitsa kwambiri pogwira ntchito ndi zitsanzo zazing'ono kapena zosinthika chifukwa zimathandiza kuwunika kwawo konse.

Kubwezeretsa Chikondwerero:

Izi zophatikizira zimathandizira mwachangu komanso moyenera pakuwunika pambuyo pakuwunika. Pipette kapena kuyika zitsanzo zanu molunjika kuchokera pansi pa chonyowa chochepetsera pochita ndi zitsanzo zamtengo wapatali kapena zamtengo wapatali. Izi ndizothandiza kwambiri pogwira ntchito ndi zitsanzo zochepa zomwe sizingawonongeke.

Kuchepetsa kwa voliyumu yakufa:

Magalasi amagalasi amathandizira kuchepetsa kuchuluka kwa mabizinesi - kapena gawo la zitsanzo zomwe zatsala pambuyo pa kusanthula - pokonzanso chikondwerero chokwanira cha chromatotography. Mapangidwe awo amakulitsa chitsikitso cha zitsanzo zolondola.

Kodi mukufuna kuphunzira zambiri za zokambirana za HPLC? Lowani m'nkhani yodziwitsa iyi kuti mumve bwino kwambiri:Kuyika kwa HPLC: Kulimbika Kuwongolera ndi Kukhulupirika

Kugwirizana:

Makina ogwiritsira ntchito magalasi amagwirizana ndi makina ambiri a Autosamamblers ndi jakisoni omwe amagwiritsidwa ntchito mu chromatography, akutsimikizira ntchito yodalirika yamalamulo ndi opanga. Mapangidwe awo okhazikika amatsimikizira zosankha zosasangalatsa kuti mukwaniritse kusintha kwa zida zosiyanasiyana.

Chitetezo chachitsanzo:

Kuphatikiza pa kupewa zitsanzo za EXPOTION, magalasi olumikizirana amapereka zitsanzo ndi chitetezo chowonjezera, kusunga malo otetezeka ndikukhala oyera nthawi yosungirako ndi kusanthula.

Mapulogalamu osiyanasiyana:

Magalasi OkhazikikaItha kupezeka mitundu yosiyanasiyana ya cromatography, monga chromatography (GC), madzi a chromatography (LC) ndi Hermatography (HPLC), ndikuwapangitsa kukhala oyenera kuthana ndi zovuta zingapo zowunikira.

Kuwongolera kutentha:

Kusanthula kwa chromatograph nthawi zambiri kumadalira zitsanzo zozama, kupanga galasi kumayambitsa njira yabwino kwambiri yowongolera kutentha kwawo pakuwunika. Yogwirizana ndi njira zowongolera, makina agalasi omangira amalola kuti kutentha kwa kutentha kwa kutentha panthawi yowunikira.

Kugwira Mosavuta:

Magalasi ophatikizika ndi ogwiritsa ntchito, monga mawonekedwe awo osavuta amawapangitsa kuti azichita bwino kutsatsa ndi kukweza mumiyendo. Kugwiritsa ntchito kwawo kumachepetsa chiopsezo pakukonzekera njira zotsatsira kubereka poyesa.

Mtengo wokwera mtengo:

Magalasi ophatikizira amapereka mapindu ambiri osagwiritsa ntchito ndalama zosafunikira, kupereka ofufuzawo kuti agwiritse ntchito makina osinthika komanso magwiridwe owunikira popanda kuwononga ndalama zambiri.

Zosankha Zamitundu:

Opanga ambiri opanga magile amapereka njira zosinthira zomwe zimapangitsa ofufuza kuti asankhe zoyikamo, monga zigawo za septa kapena zokuthandizani, zomwe zimakwaniritsa zofuna za chromatographic.

Maganizo a Zachilengedwe:

Galasi ndi nkhani yosavuta yobwezeretsa mosavuta, motero kusankha makalasi ogwiritsira ntchito ngati gawo la machitidwe a eco-rocectory amamveka kuchokera ku chilengedwe. Kuchepetsa zinyalala za pulasitiki zolumikizidwa ndi zoyika zotayika ndi gawo linanso loti mugwiritse ntchito labotale.

Kukonzanso umphumphu:

Magalasi olumikizirana amayika akuwonetsetsa kuti akuphatikizidwa ndi katundu wa zitsanzo zosasunthika nthawi yosungirako ndikuwunika zotsatira zolondola, zodalirika. Izi ndizofunikira kuti mukwaniritse kusanthula kolondola komanso kwapadera.

Mapeto

Mwa chromatography.Magalasi Okhazikikaadzitsimikizira kuti anali ndi zida zolimbikitsira chifukwa cha kuthekera kwawo kukonza kuwongolera, kuchira ndi chitetezo. Ofufuzawo ndi ofufuza ochokera m'makampani osiyanasiyana amathamangira chifukwa cha kusiyanasiyana kwawo, kugwirizana ndi kugwiritsidwa ntchito mosavuta; kuwapangitsa kukhala katundu wofunikira mu njira zamakono. Monga zovuta zowunikira zimapitilira kukula mu zovuta, makalasi magalasi okhalamo omwe amatsimikizira zotsatira zasayansi.

Mukuyang'ana mayankho okwanira okhudza HPLC Vials? Onaninso - Onani nkhani iyi pazinthu zonse zomwe mukufuna:50 mafunso ambiri omwe amafunsidwa kawiri pa HPLC Mbale

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