Kodi mukudziwa za Thromatography?

Pepala chromatography ndi njira yogwiritsidwa ntchito kwambiri yomwe imalekanitsa ndikuyizindikiritsa zinthu zosakanikirana za zinthu zina zochokera pagawo lawo la gawo (pepala) ndi gawo lam'manja (zosungunulira). Ndizothandiza kwambiri mu chemistry ndi biocheminry yowunika zinthu ngati utoto, ma amino acid, ndi zina zopangidwa zina.

Kodi Thromography ndi chiyani?

Pepala chromatography ndi mtundu wamadzimadzi a chromatograph komwe gawo la station ndi pepala la zosefera ndi gawo la foni ndi zosungunulira zomwe zimatuluka kudzera pa pepala la Fsefesa. Njirayi imadaliranso gawo lina la zinthu pakati pa gawo la station (pepala losefera) ndi gawo lam'manja (losungunulira). Monga zosungunulira zimasunthira pepala la zosefera ndi capillary, limanyamula zigawo za osakaniza mosiyanasiyana mosiyanasiyana malinga ndi zomwe akuwagwiritsa ntchito.

Mfundo zoyambirira za ma chromatographzographraphraphraphramography imatha kumveka bwino.

Gawo chromatography: Mu mtundu uwu, zinthu zimagawidwa pakati pa magawo awiri amadzimadzi. Gawo la station lili ndi madzi m'mapapo pepala lazosefera, pomwe gawo lam'manja ndi losungunulira lomwe limayenda kudutsa m'ma pores. Kulekanitsidwa kumachitika chifukwa mankhwala osiyanasiyana amakhala ndi maudindo osiyanasiyana kwa magawo oyimilira ndi mafoni.

ADSORARDEPARTOGOGY: Pano, nthaka yolimba ya pepalali imachitika ngati gawo la siteshi ndipo madzi solive amagwira ntchito ngati gawo lam'manja. Zigawozo mu osakaniza zimalekanitsidwa kutengera adsorption yawo yolimba ndi kusungunuka kwawo mu gawo lamadzi.

Zida

Gawo la Statiary: Pepala la chromatographic, lomwe limapangidwa ndi cellose. Imapereka maziko a zinthu zofunika kuti azitsatira.

Gawo lam'manja: zosungunulira kapena kusakaniza kwa ma soldents omwe amasuntha papepala ndi capillary. Kusankha zosulira kumatha kukhudza kwambiri kudzipatulira.

Sampy: Kusakaniza kuwunikidwa, nthawi zambiri kumawonedwa pamalo oyambira papepala.

Ndikufuna kudziwa zambiri za kukonzekera kwa HPLC, chonde onani nkhaniyi: Mayankho a HPLC Kukonzekera kwa Zotsatira Zabwino

Mapepala a chromatography

Kukonzekera: Sankhani pepala lalikulu losefera ndi chidwi winawakenso kuti mupatse kupatukana kogwira mtima. Ikani gawo laling'ono la chisakanizo cha zilembo za zilembo za chromatography.

Kukonzekera zitsanzo: Sungunulani osakaniza ndi gawo loyenera ngati gawo la gawo lam'manja.

Kuwona: Gwiritsani ntchito capillary kapena tizilombo tating'onoting'ono kuti muchepetse njira yocheperako papepala lazosefera.

Kukula: Viyikani m'mphepete mwa pepala loyeserera kukhala lopanga (gawo lam'manja). Monga momwe ntchito yama capillary imachitika, zosungunulira zimakwera pepala loyesa, kunyamula zigawo zina ndi izi.

Kupatukana: Monga kusungunuka, zinthu zosiyanasiyana za zitsanzozi zimasunthira pamitengo yosiyanasiyana chifukwa chogwirizana ndi gawo la station ndi kusungunuka mosiyanasiyana mu gawo la foni.

Kuwona: Pambuyo pa solvent kutsogolo kwasunthira mtunda wokwanira, pepalalo limachotsedwa kuchokera ku zosungunulira ndikusiyidwa kuti ziume. Zigawozi zimatha kuwona pogwiritsa ntchito kuwala kwa UV, ma reagents mankhwala, kapena poyang'ana kusintha kwamtundu.

Kusanthula: mtunda wodutsa mdera lililonse ndi zosungunulira kutsogolo kwake amayesedwa kuti awerengere mtengo wa RF (chisoti) chozindikiritsa.

Mapulogalamu a Thromatography

Pepala chromatography ili ndi ntchito zingapo m'magawo osiyanasiyana:

Biochemistry: Kusanthula kwa amino acid, shuga, ndi biocolecles ena.

Mankhwala ogulitsa: Kuzindikiritsa zosakaniza ndi zodetsa zopangidwa ndi mankhwala osokoneza bongo. Amagwiritsidwa ntchito kuzindikira mankhwala ogwiritsira ntchito ndi zodetsa mu mankhwala osokoneza bongo pakukula ndi kuwongolera kwapadera.

Sayansi Yachilengedwe: Pepala chromatography imagwiritsidwa ntchito kuzindikira zodetsa m'matumba amadzi ndikusanthula zodetsa mu nthaka.

Makampani Ogulitsa Chakudya: Njirayo imathandizira pakusanthula zowonjezera, zodetsedwa, ndi colorants yopeza kuti zitsimikizire kutsatira malamulo otetezeka.

Zabwino zamapepala chromatography

Kugwira Ntchito Kwakukulu: Zimafunikira zida zochepa komanso zida zochepera poyerekeza ndi njira zina za chplc (zolimbitsa thupi zamadzimadzi). Kuphweka: Njirayi ndiyosavuta ndipo imatha kuchitidwa ndi zida zoyambira labotale, kotero zitha kugwiritsidwa ntchito pophunzitsira.

Njira Yotsika Yotsika: Chitsanzo chochepa chabe chomwe chikufunika kuti musanthule, ndikupangitsa kukhala bwino pa zitsanzo zamtengo wapatali kapena zochepa.

Kusiyanitsa: Itha kulekanitsa zinthu zosiyanasiyana kutengera mankhwala, kuphatikizapo polar ndi colar

Zoperewera za Thromatography

Ngakhale ndizabwino zake, mapepala a chromatotoragraphy ali ndi malire ena:

Kutha Kochepa: Nthawi zambiri oyenera mavidiyo ang'onoang'ono.

Kusintha: Kuthetsa kusanduka sikungakhale kwakukulu monga momwe zimakhalira ndi njira zapamwamba kwambiri monga HPLC kapena gasi chromatography (GC).

Kuwononga Nthawi: Chifukwa cha kusamukira pang'onopang'ono, njirazi zingatenge nthawi yayitali poyerekeza ndi njira zina za chromatographic.

Kusanthula Kusanthula Kwambiri: Ngakhale zotsatira zabwino zimatha kupezeka mosavuta, njira zowonjezera zodziwika bwino zingafunike kuti muthe kuwerengera.

Mapepala a chromatotoragraphy amakhalabe njira yofunika kwambiri yolekanitsa ndi kusanthula zozimitsa paminda yosiyanasiyana ya sayansi. Kuphweka kwake, kugwiritsa ntchito bwino mtengo, komanso kuthekera kopatsa zinthu zofunikira kumapangitsa chida chofunikira cha akatswiri azamiliyoni ndi ofufuza. Ngakhale kuti anali ndi malire ake poyerekeza ndi njira zapamwamba kwambiri, zimagwiritsidwabe ntchito kwambiri pazophunzitsira komanso kusanthula koyambirira kwa labotaries.

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