Kodi mukudziwa za matenda a Silanzaization a HPLC Vial?

Silnuzation ya HPLC MbaleNdi njira yovuta yopangidwira magwiridwe antchito a chromatographic pochepetsa kutsatira zomata za ziwonetsero zagalasi. Izi zimapindulitsa kwambiri pakuwunika kochuluka, makamaka pochita ndi zitsanzo zambiri, monga momwe zimatsalira zilizonse zomwe zingakhudze kwambiri zotsatira zake. Nkhaniyi ikupereka mwachidule za kusawala, zake, magwiridwe ake, magwiridwe antchito, ndikuganizira kuti mugwiritse ntchito moyenera kwambiri ma chromatography.

Kodi Silalembeti ndi Chiyani?

Kusanja kumafuna kugwiritsa ntchito silane yofiyira pamtunda wagalasi lagalasi. Izi zimathandizanso ma hydroxyl aulere (Silnol) pagalasi, yomwe imadziwika kuti imalumikizana ndi mapengwe osiyanasiyana, makamaka polarku. Mwa kusilira masamba awa, kusindikizidwa kwambiri kumachepetsa adsororption ya owunikira ku makoma a vial, ndikuwonetsetsa kuti kubwezeretsedwa kwake ndi zotsatira zowona. Mu HPLC Mbale, mankhwalawa amapeza magulu a Silanol (SI-O) pamkati mwa galasi la ma borosilte. Magulu awa hydroxyl amatha kuyanjana ndi polar, akutsogolera kutsanzira ndi kutaya pakuwunika.

Njira ya Silanurization

Njira ya Silanurization imaphatikizapo izi:

Kuyeretsa: Choyamba, chotsani galasi pogwiritsa ntchito zosungunulira monga acetone kuti muchotse zodetsa zilizonse zomwe zingasokoneze kusokonekera.

Kugwiritsa ntchito silane: Wothandizila milungu ya methy adayambitsidwa pamtunda wa vial ya Vapor. Wothandizirayo amakhudzana ndi magulu a hydroxyl kuti apange molunjika mwamphamvu zomwe sizimagwira komanso zosasangalatsa.

Kulumikizana kwa Coovale: Silane amachita ndi magulu a hydroxyl pagalasi kuti apange chipongwe cha hydrophobic, kuchepetsa nkhawa ndikuletsa ziyeso kuti zisatsatire makoma a vial.

Kuchirikiza: Mabotolo omwe amachitiridwatsidwa amachiritsidwa kuti atsimikizire kuti silane amapanga chomangira chagalasi pachimake, potero chikuwonjezeka kukhala ndi mikhalidwe ina yomwe mwakumana ndi ma chrototography.

Zotsatira zake ndi vial okhala ndi malo osinthika omwe amachepetsa kulumikizana ndi mankhwala a polar, kukonza chiwonetsero chachitsanzo ndi kuwunikira.

Ubwino wa Silnuzation

Kugwiritsa Ntchito Mbale Za Silancyc HPLC imapereka zabwino zambiri:

1. Sinthani chiani kuchira

Mbale za ku Silanchired zitha kuchepetsa kwambiri kutsatira kwa zionetsero za makoma a vial. Izi ndizofunikira kwambiri kuti pasanthulidwe pang'ono, pomwe kuchepa kwa zitsanzo zochepa kungakhudze zotsatira za zotsatira. Poletsa adsorption, mipanda ya Silanchired Press Press Kubwezeretsa Kwambiri.

2. Konzani kulondola

Izi zimatsimikizira kuti zotsatira sizikhudzidwa ndi zotsalira zamiyo, poyankha zolondola za kuchulukana zomwe ndizofunikira kwambiri pakuwunika komwe kumayambitsa kafukufuku.

3. Kugwirizana ndi Kusanthula Kwambiri

Mbale za Silanted ndioyenera kugwiritsa ntchito mitundu yosiyanasiyana, kuphatikizapo zomwe zimakhudzana ndi polar ndi hydrophobic mankhwala. Amakhala oyenera makamaka kwa mankhwalawa, ma biopolymers ndi zinthu zina zokhazikika zomwe zitha kulumikizana ndi zigawenga zagalasi.

4. Moyo wautali

Njira yokhotakhota ya Vaporotion Staturization imatulutsa zokutira zokhazikika zomwe zimasungidwa pakapita nthawi. Maonekedwe a Silaniated amakhalabe okhazikika kwa nthawi yayitali ngakhale atakhala ndi ma sol sol sol ndipo ndi oyenera kusungidwa kwa nthawi yayitali.

Mapulogalamu a Silanized HPLC Mbale

Mitengo ya Silanc HPLC itha kugwiritsidwa ntchito m'minda yosiyanasiyana:

1. Kusanthula Mankhwala

M'malonda opangira mankhwala, Mbale Za Silanchired amagwiritsidwa ntchito:

Kuyeserera kwatsimikizika: onetsetsani kuti mankhwalawa amakhazikika panthawi yosungirako komanso kusanthula.

Kupenda Kwambiri: Zimathandizira kuyeza molondola mankhwalawo mankhwalawa.

2. Kuyesa kwachilengedwe

Mbale izi ndizofunikira pakuwunika kwachilengedwe:

Kuyesa kwamadzi: Kanisani zitsanzo zamadzi pazinthu zodetsa pomwe mukuchepetsa zotayika.

Kusanthula kwa dothi: Konzani zowonjezera za dothi kuti zisanthulidwe kena popanda kutaya mayeso.

3. Kuyesa kwa Chitetezo cha Zakudya

Muzosankha za chakudya:

Kusanthula kwa mankhwala osokoneza bongo: onetsetsani kuti mwapeza kuchuluka kwa mankhwala osokoneza bongo popewa kutaya kwake panjira.

Kuyeserera kwa Micriright: Pendani zitsanzo za zakudya zokhala ndi michere popanda kusokonezedwa ndi zinthu zoyipa.

4. Kufufuza ndi Kukula

Mu kafukufuku:

Kukonzekera zitsanzo: kumapereka njira zodalirika zosungira zitsanzo zingapo zisanachitike.

Ntchito zoyeserera: zimathandizira kuyesa komwe kumafunikira zinthu zomwe zimafunikira mikhalidwe.

Kusamala mukamagwiritsa ntchito Mbale Za Silanized

Ngakhale Mbale Zopusa Amapereka Zabwino Zambiri, Pali zinthu zofunika kuzidziwitsa:

1. Sankhani mtundu woyenera wa botolo

Ndizofunikira kusankha mtundu wolondola wa Vial Vial pazosowa zanu. Zinthu monga kuchuluka kwa zitsanzo, kuyerekezera kwa mankhwala, komanso chidwi chomwe mukufuna kumayenera kuwongolera.

2. Kusamala kuti mugwiritse ntchito

Mankhwala oyenera amachititsanso kukhulupirika pamwamba pa zilankhulo:

Pewani kulumikizana mwachindunji ndi malo amkati kuti musapidwe.

Valani magolovesi oyera mukamagwiritsa ntchito Mbale kuti muchepetse kusintha kwatsalira.

3. Kugwirizana ndi ma sodi

Ngakhale malo oyimilira omwe amakhala osagwirizana nawo, ndikofunikira kutsimikizira kuyerekezera ndi mankhwala omwe amagwiritsidwa ntchito popenda kusanthula kapena kuwonongeka kwa magwiridwe antchito.

Njira Zina

Ngakhale Silanrization ndiwothandiza, pali zosankha zina monga zochepetsera zoyambira (RSA). Mitengo ya RSA imapangidwa kuti ikhale yotsika kwambiri ndipo musadalire zofunda za Silane. Njira imeneyi imatha kupereka zotsatira zokhalapo mosasinthasintha ntchito zina pomwe zilankhulo zikuluzikulu zitha kulephera chifukwa chosakwanira kapena kuchepa kwa nthawi.

Ngati mukufuna kudziwa zambiri za Siconid HPLC Mbale, chonde dinani nkhaniyi:"Kuwonetsa kwa Siliconated HPLC Vials"

Vilunza Mbale za HPLC ndi njira yofunika yomwe imathandizira kuyeserera kowunikira mwakuwunikira kusanthula ndikulimbana ndi zitsanzo. Posintha mipata yamkati yamagalasi yamagalimoto, mankhwalawa amatsimikizira zolondola, zodalirika zoyeserera zosiyanasiyana m'magulu ogulitsa, chitetezo cha chakudya, komanso zofuna kufufuza. Mwa kumvetsetsa zabwino komanso kugwiritsidwa ntchito moyenera komwe kumalumikizidwa ndi Mbale Zosagwiritsa Ntchito, ma laboratoes amatha kuonetse bwino makongoletsedwe awo pokhalabe abwino komanso olondola chifukwa cha zotsatirapo. Monga ukadaulo wa Smilation akupitilizabe, ofufuza angayembekezere kupitiriza kupitiriza ntchito mwanzeru ndi ntchito zosiyanasiyana kudutsa maphunziro a sayansi.