Oct. 16th, 2024
Chromatography ndi njira yogwiritsiridwa ntchito kwambiri pamankhwala owunikira, biochemistry ndi njira yodziwika bwino, biocheminry yogwiritsidwa ntchito, komanso minda yosiyanasiyana yolekanitsa ndi kusanthula zinthu mu osakaniza. Mbali yofunika kwambiri ya cromamography yomwe nthawi zambiri imanyalanyazidwa ndi kukula kwa vial yomwe imagwiritsidwa ntchito kuti igwire zitsanzo. Kusankha kwa vial kukula kungakhudze momveka bwino, kubereka, komanso mtundu wonse wa zotsatira za chromatographic. Nkhaniyi ikuwunikira zosiyanasiyana za kukula kwa chromatography, zomwe zimayang'anitsitsa zitsanzo, zotsatira zoyipa, chiopsezo choipitsidwa, komanso kulingana ndi maluso owunikira.
Kuganizira Kwambiri
Kuchuluka kwa zitsanzo zomwe zimasandulika ndi imodzi mwazinthu zazikulu zomwe zimapangitsa kusankha kwa mitundu yosiyanasiyana. Mitengo ya chromatography imapezeka mumitundu yosiyanasiyana, imayamba kuchokera ku micro Mbale (0.3 ml) ku miyeso yokhazikika (1.5 ml ndi 2 ml).
Miphika ya micro: Awa ndi abwino kwa ma voliyumu ang'onoang'ono ndipo nthawi zambiri amagwiritsidwa ntchito kwambiri pogwiritsira ntchito chidwi kwambiri monga ma chplutography ndi gasi. Kukula kwake kakang'ono kakang'ono kumachepetsa zinyalala ndikuloleza kusanthula kolondola pamene kupezeka kwachitsanzo ndi kochepa.
Mbale Zosiyanasiyana: Mbalezikulu kwambiri amakhala ndi mayunitsi okulirapo koma amatha kubweretsa mavuto ngati silingafanane ndi zitsanzo. Matumbo ochulukirapo amatha kusintha kapena kuwonongeka kwa mankhwala osasunthika, omwe amatsogolera ku zolakwika.
Kusankha Vial yemwe amafanana ndi buku lomwe likufunika chiwonetsero chimatsimikizira kuti njira yachilengedwe imagwirizani ntchito mokwanira mukamasungabe chidwi.
Ndikufuna kudziwa chifukwa chake mipata ya cromography ndiyabwino kuposa Mbale zapulasi, chonde onani nkhaniyi: Zifukwa zitatu zapamwamba zomwe zimapangidwira miyala chromatography ndiyabwino kuposa Mbale zapulasi
Zotsatira Zam'mutu
Pamutu chimatanthawuza malo omwe ali ndi vial pamwamba pa makina amadzimadzi. Matumbo oyenera ndiofunikira kuti asanthule mosalekeza panthawi yowunikira.
Ziwopsezo za Evaptoation: Ngati vial ili ndi mutu wambiri wogwirizana ndi kuchuluka kwa zitsanzo, zomwe sizingasinthe zitha kusintha, kusintha magawo andende komanso kuphwanya magawo. Izi ndizofunikira makamaka mu ntchito ya GC komwe kukhazikika kwamphamvu kumasanthula.
Kusasinthika kwa jekeseni: Kusakhazikika kwa jakisoni kumatha kubuka mogwirizana ndi kuwongolera kosayenera. Kuonetsetsa kuti kukula kwa vial ndikoyenera kuphindutsira zitsanzo zitsanzo kumathandizanso kukhalabe okhazikika mkati mwake, zomwe zimapangitsa jakisoni wobereka.
Zoopsa zodetsa nkhawa
Zinthu ndi kukula kwaMbale za chromatographyamathandiziranso zoopsa zodetsa nkhawa. Zipangizo zosiyanasiyana zimawonetsa magawo osiyanasiyana ogwirizana ndi zitsanzo.
Kusankha Zinthu: Mbale zamagalasi nthawi zambiri zimakonda chifukwa cha omwe amadwala matendawa, omwe amachepetsa zoopsa zochokera kuzinthu zoyambira. Komabe, Mbale zapulasitiki zitha kukhala zoyenera mapulogalamu enaake, monga kuyesa kwa PFAS. Kusankhidwa kwa zinthu kuyenera kugwirizanitsa ndi mtundu wa mapangidwe oyesedwa.
Padziko Lonse: Mateleni ndi ma acid a acid amatha ADSORB patali kwambiri, zomwe zimapangitsa kuti zichepetse mitengo yobwezeretsa ndi njira zochepetsera. Mwachitsanzo, mapuloteni a hydrophobic amatha kupanga mchere ndi galasi pakapita nthawi, kusanthula kosalekeza. Kuti muchepetse mavuto awa, apadera a Adsorption Mbale za Adsorption kapena polypropylene Mbale zopangidwa kuti amveke bwino biomoleclules akulimbikitsidwa.
Kugwirizana ndi Njira Zowunikira
Maluso osiyanasiyana a cromatography angafunike kumafunikira kukula kwa vial ndi mitundu kuti awonetsetse bwino.
Malangizo a HPLC ndi GC: Zotsekereza val styles (screw caps vs. nsonga za romp) zimatha kukhudza kwambiri pakusanthula pakuwunika.Chipewamnthawi zambiri amagwiritsidwa ntchito mu Mbale za GC ku chisindikizo mosabisa, pomwescrew capsZitha kukhala zoyenera kugwiritsa ntchito ma HPLC pomwe nthawi yofikira ndiyofunikira.
Mapulogalamu apadera: Kuwunika kwa biokharmaceutical, Mbale zina zimapangidwa kuti zichepetse adsorption ndikuteteza ofufuza mwaluso ngati mapuloteni ndi oligontuotides. Mwachitsanzo, thermo sayansi ya sayansi ya golide imakhala ndi galasi lotsika la Adsorption lomwe limathandizira kuti abwezeretse mitengo yoyambira.
Mukufuna kudziwa momwe mungasankhire kachilombo ka HICP VS. SNAP VS. Conct Cap Vial? Onani nkhaniyi: CR. CRAIL VS. SNAP VS. Screct Cap Vial, momwe mungasankhire? \ / ndikufuna kudziwa momwe angasinthire mbale za HPLC?
Kusintha kwa zotsatira zowunikira
Zotsatira zokulira kukula kwa vial pa kusanthula kwa chromatographic kumatha kuyambitsa kusintha kwakukulu pamachitidwe owunikira.
Kulondola: Kugwiritsa ntchito Mbale zoyenerera moyenerera kumachepetsa chiopsezo cha kuipitsidwa ndikuwonetsetsa kuti zotsatira zake zimakhala zosasintha. Mbale zapamwamba kwambiri zimachepetsa kusiyanasiyana chifukwa cha zinthu zakunja, potengera kulondola.
Kuletsa: Kuwongolera mosasintha mosasintha komanso kusankha koyenera kumathandiza kukwaniritsa zotsatira zoberekera. Kusintha kwa jakisoni chifukwa cha kukula kosayenera kumatha kutanthauzira mosagwirizana.
Kukhuta: Mbale zazing'ono zomwe zidapangidwa kuti zisakhale ndi kuchuluka kwa voliyumu yotsika kumathandizanso kumverera bwino pokulitsa kuchira ndikuchepetsa. Izi ndizofunikira kwambiri pofufuza zitsanzo zamtengo wapatali kapena zochepa.
Mapeto
Mphamvu ya kukula kwa vial pa crobomagraphygzographygraphy imapangitsa kuti pasamizimini muyezo komanso wovuta kupeza zotsatira zolondola komanso zodalirika. MukamasankhaMbale za chromatography, kulingalira mozama kuyenera kuperekedwa kwa zofunikira za bukuli, kuyendetsa bwino mawu, zoopsa zodetsedwa zokhudzana ndi zida zosiyanasiyana, komanso kugwirizana ndi njira zingapo zowunikira. Mwa kumvetsetsa zinthu izi ndikusankha vial yoyenera pakufunsira kwinakwake, ofufuzawo amatha kukonza mawonekedwe awo a cromatotography.
Pomaliza, ngakhale pogwiritsa ntchito HPLC kapena GC Systems, kapena kusanthula zovuta za Biomuleculec mu kafukufuku, kusankha mawonekedwe oyenera a chromatography ndikofunikira kuti muwonetsetse bwino ntchito komanso kupeza zotsatira zabwino kwambiri.
Ndikufuna kudziwa zambiri za momwe angayeretsere ma chromatography y, chonde onani nkhaniyi: Zothandiza! Njira 5 zoyeretsa ma chromatography
Achizungu
Zauchina
