Mafuta a chromatography - misa sportmetry (GC-MS) ndi njira ziwiri zamadzi zowunikira zomwe zimagwiritsidwa ntchito kudzipatula, zindikirani, komanso kuchuluka kwa zitsanzo. Njira iliyonse ili ndi maubwino ake apadera ndipo ndiyoyenera kuwunika mitundu. Kumvetsetsa kusiyana pakati pa GC-MS ndipo HPLC ndikofunikira posankha njira yolondola yochokera ku mtundu wa zitsanzo ndi zofunikira zina.

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Kusiyanitsa pakati pa GC-MS ndi HPLC

1. Gawo la foni

Kusiyana kwakukulu pakati pa GC-MS ndi HPLC ndi gawo lam'manja. A GC -ms amagwiritsa ntchito gawo la mafoni a GC Izi zimapangitsa GC-MS makamaka yoyenera kusanthula zinthu zosasunthika zomwe zimatuluka mosavuta pamatenthedwe kwambiri.

Mosiyana ndi izi, HPLC imagwiritsa ntchito gawo lamadzimadzi, nthawi zambiri zosungunulira zosungunulira zogwirizana ndi polarity ndi kusungunuka kwa zitsanzo. Izi zimapangitsa HPLC kusanthula mitundu yosiyanasiyana, kuphatikizapo zonse zosagwirizana komanso zosagwirizana.

2. Mtundu wachitsanzo

Mitundu ya zitsanzo zomwe zingasanthuledwe ndi luso lililonse lachitatu limasiyana kwambiri. GC-MS imayenererana ndi kusanthula kosasunthika kapena osasunthika, monga ma hydrocarbons, mafuta ofunikira, ndi zodetsa zachilengedwe. Sizothandiza kwenikweni kwa kutentha kwa kutentha kapena kusakhala kosasunthika. Komabe, kugwirizira zitsanzo zingapo, kuphatikizapo polar, biomolecules, mankhwala opangira mankhwala, komanso zosakhazikika zovuta zomwe zingakhale ndi mchere. Kuchita motsutsa kumeneku kumapangitsa kuti HPLC Sankhani yapamwamba m'minda monga wa biochemistry ndi mankhwala.

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3. Mitundu ya kutentha

Kutentha kumachita mbali yofunika kwambiri m'maluso onse, koma m'njira zosiyanasiyana. GC-MS imagwira ntchito motentha kwambiri, makamaka pakati pa 150 ° C ndi 300 ° C, kuti muwonetsetse bwino za chitsanzo. Kutentha kwambiri kotereku kumalola kusanthula mwachangu, koma kumachepetsa mitundu ya zitsanzo zomwe zingasanthulidwe, monga matenthedwe okwanira kutentha amatha kuwonongeka. Mosiyana ndi izi, HPLC imatha kutentha kwamphamvu kapena pang'onopang'ono, ndikupangitsa kuti ikhale yoyenera kupenda matenthedwe othamanga popanda chiopsezo chowonongeka.

4.. Kulekanitsa

GC-MS ndi HPLC ili ndi njira zosiyana siyana chifukwa cha magawo osiyanasiyana. Mu GC-ms, kudzipatula kumakhazikika makamaka pa kusakoloka kwa mankhwala; Zovuta zochepa zosasunthika zimalumikizana kwambiri ndi gawo la station ndikulosera pang'onopang'ono kuposa mankhwala osasunthika.

Mosiyana ndi izi, HPLC imasiyanitsa mankhwala kutengera momwe iwo amayendera ndi magawo a mafoni ndi okhazikika, omwe amatsimikiziridwa ndi zinthu monga polarity komanso kusungunuka. Makina ophatikizika nthawi zambiri amasunthira pamtunduwu mwachangu chifukwa amakopeka ndi gawo lam'manja.

5. Njira zodziwika

Njira zomwe zimagwiritsidwa ntchito pantchito yolembedwa ndi GC-MS ndipo HPLC ndizosiyananso. GC -Ms amaphatikiza gasi chromatography ndi misa yamafuta, yomwe imalola kuzindikiritsa pang'ono ndi kuzindikirika kwa mankhwala kutengera kuchuluka kwa magalimoto ambiri atalekanitsa. Kuphatikiza uku kumapereka chidziwitso mwatsatanetsatane zokhudzana ndi kusanthula. Motsutsana,HplcNthawi zambiri amagwiritsa ntchito uV-wowoneka wowoneka bwino kapena wojambula, zomwe zimayesa momwe zitsanzo zimagwirizira opepuka kapena kusinthika kukhala kopepuka momwe imakhalira kudzera mu chojambula. Ngakhale njirazi zimathandiza kugwiritsa ntchito magwiridwe ambiri, amatha kudziwa zambiri kuposa zowoneka bwino kwambiri.

6. Zida ndi mitengo

Zida zofunika za GC-MS ndi HPLC zimasiyananso malinga ndi zovuta zomwe zimachitika. Makina a GC nthawi zambiri amakhala osavuta; Amafuna kuti mafuta onyamula (onyamula mpweya) koma osati pampu yayitali kwambiri chifukwa magses ali ndi mamasukidwe otsika kuposa amamwa. Izi nthawi zambiri zimapangitsa kuti GC Sysy imatsika mtengo kuti igwire ntchito nthawi yayitali. Mosiyana ndi izi, HPLC Systems imafuna kampu yokwera kwambiri kuti ikanitse madzi osungunuka kudzera mu gawo lodzaza ndi gawo lokhazikika, ndipo ndilovuta kwambiri ndipo mtengo wake ndi wokwera kwambiri chifukwa cha kufunika kwa kufunikira kwa ma solfordenti yapadera.

Kusankha pakati pa GC-MS ndi HPLC

Mukasankha kugwiritsa ntchito GC-MS kapena HPLC, pali zinthu zingapo zomwe muyenera kuziganizira:

Chikhalidwe cha sampu yanu: Dziwani ngati zitsanzo zanu sizikusintha kapena zopanda pake.

Kukhazikika kwa matenthedwe: kuwunika ngati anzanu akhoza kupirira kutentha kwambiri popanda kuwonongeka.

Zofunika kukhuta: Ganizirani ngati mukufuna chidziwitso chatsatanetsatane (chomwe chimakonda GC-MS) kapena muyeso wongoyang'ana (zomwe zingachitike ndi HPLC).

Zopinga: Yesetsani bajeti yanu yogula zida ndi kukonza.

Mwachidule, GC-MS ndi HPLC ndi zida zofunikira kwambiri pamacheta owunikira, ndipo njira iliyonse imakhala ndi maubwino othandizira ena. Mwa kumvetsetsa zomwe akusiyana.

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M'mbuyomu:

Kodi kusintha kwa kukula kwa vial pa crobomagraphy kumabweretsa chiyani?

Ena:

Njira zodziwika bwino za GC-ms

Ma tag: Gasiskhomographraphraphle chromatography-Mbale Chipatso Mbale-chromatography Hplcanayalysis VPLC-Mbale

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