Kukonzekera zitsanzo & kusefa: kuwonetsetsa mtundu wa chromatography

Zotengera zoyenera zoyera, chotsani zida zaluso, ndipo, ngati zingafunike, zosemphana - zimachepetsa phokoso loyambira komanso kuyikapo ma bakol. Kulephera pano kumapangitsa nsonga yolumikizira, ikuyendetsa Baselinenes, kapena kutchinjiriza.

1. Zowonongeka & matrix

Vuto

• Phokoso loyambira ndikukwera phokoso lowoneka bwino.

• Mitengo yosayembekezereka imawoneka kuchokera ku zosayera.

• Kubala Zosavomerezeka Pakati pa jakisoni.
  

  

Pangitsa

• Zotsalira mugalasi: osakwanira osakwanira masikono osatsutsika; pulasitiki amatha kupanga mavidiyo.

• Zoyeretsa: Tsatirani zolengedwa za mu Rinse solsorbed.

• Zilengedwe Zapakatikati: fumbi la lab mpweya ndi vocs Adsorb pa zitsanzo zotseguka.
  

  

Kankho

1. Kuyeretsa kokhazikika: Acid Plaeak → Akupanga madzi oyera amatsuka → nayitrogeni yowuma yagalasi; zowonjezera zoperewera za pulasitiki.

2. Kufalikira: Centrifuge pa 3000rpm kwa 10min kapena gwiritsani ntchito prefilter 5μm isanachitike filfilter Final 0,22μm.

3. Malo olamulidwa: Chitani zokongoletsera za laminar kuti muchepetse kuipitsidwa kwa mpweya.
   
   

2. Zosefera zovala kuchokera ku zosefera zosagwirizana

Vuto

• Autosampler Spams ndi singano wamba.

• Kutsika kutentha, madera osagwirizana.

• Okwezeka obisika komanso Column Tsamba la Tsamba.
  

  

Pangitsa

• Membrane Pommatch: nayiloni imatupa mu ortic sol; PVDF imakwapula pamtunda wokwera.

• Kukula kolakwika: 0.22μm zosefera Tizilombo tating'onoting'ono tokha komanso kuchepetsa kuyenda; Zosefera 0,45μm zitha kuvala ndi ma microcrystals.

Kankho

1. Membrane-sonvent matrix: Gwiritsani ntchito ma hydrophilic pes \ / MCE kuti mudzichepetse (ph4-8), hydrophobic ptfe \ / pvdf magawo a organic; Pewani ma cellulose acetate mu acid amphamvu.

2. Sankhani kukula koyeneraGwiritsani ntchito 0.45μmμm for routine HPLC, 0.22μm ya Uhplc kapena ntchito; Zitsanzo zapamwamba kwambiri za kutchuka kwa 5μm.

3. Woyang'anira ΔP: Kupanikizika kwa Track; Sinthani zosefera pomwe backpressure zimakwera> 15%.
   
   

   

3..

Vuto

• Samwali yosefedwa imawoneka mitanda ya mitambo kapena imanyamula tinthu tating'onoting'ono.

• New Couse nose kapena Ghost Peaks positi.

• Kuchira kuchepetsedwa.
  

  

Pangitsa

• Kutupa: Thf imatupa potfe, kusintha mawonekedwe a pore ndi kuyenda.

• Membrane Wopezedwa: Nylon mu mawonekedwe acidic amatulutsa oligomer omwe amamwa pa 214nm.

Kankho

1. Mayeso ogwirizanaSoust Yosaloŵero Yosoweka pa 24h pa 25 ° C; Yenderani zotupa kapena zolemera.

2. Yemwe Ayese Mayeso a LC-MS: Yerekezerani zitsulo zokwanira za vs osefera.

3. Gwiritsani ntchito zosefera zochepa: Akupanga-welded Ptfe kapena ma pes osamatira.
   
   

Njira Yotsogola: Kuyang'anira Kuyang'anira

Chitetezo

Sinthanitsani ma sps oyeretsa, kusefa, ndi kusankha kwa membrane kuti mupewe zolakwa.

Kuwunikira

Kujambulitsa Kuthamanga, Kubweza, ndi zitsanzo zopanda kanthu ku Balselnes kuti muzindikire.

Kukhoza

Khalani ndi kusanthula kwa mtengo wolakwika kuti mupange kuzindikirika ndi kukonza.

"Kusanthunza kumayamba ndi kugwirira ntchito tinthu tating'onoting'ono."