Chromatography ndi njira yofunika kwambiri yopezeredwa yomwe imagwiritsidwa ntchito kwa labotorees pazowunikira zingapo zowunikira, kuphatikizapo kuzindikiritsa, kulekanitsa, komanso kuchuluka kwa mankhwala a mankhwala omwe alipo mu zosakaniza zovuta. Kuonetsetsa zotsatira zolondola komanso zodalirika zolondola, kusankha mikwapulo ya 18mm - kukula kofala kudutsa ntchito zab - ndikofunikira. Apa tikuwunika atatu mwa malingaliro akuluakulu mukamasankha kukula kwa vial.

1. Zipangizo ndi kugwirizana kwa mankhwala

Kuganizira zakuthupi mukamasankha18mm crematographyndi chimodzi mwazofunikira kwambiri. Zosankha wamba zimaphatikizapo galasi ndi mapulasitikisi osiyanasiyana ngati polypropylene ndi polyethylene; Mukamasankha Mbale Zoyesa zanu ndizofunikiranso kuti azifanana ndi zitsanzo ndipo ma sol sol amagwiritsidwa ntchito.

Mbale zagalasi:Mbale zagalasindizabwino pazogwiritsa ntchito zokhudzana ndi zitsanzo zamagetsi zamagetsi kapena zisungunuke, popeza ndizopepuka ndipo zimatha kulolera kutentha komanso zachilengedwe. Akhozanso adsorb pa pulasitiki yomwe imapangitsa kuti ikhale yosavuta.

Mbale zapulasitiki:Mbale zapulasitikiPezerani zopepuka komanso zosokoneza, zimapangitsa kuti akhale njira yabwino yogwiritsira ntchito mapulogalamu ena. Komabe, ofufuza ayenera kuonetsetsa pulasitiki yawo yosankhidwa ndi ma sol soltto kapena zitsanzo zomwe zimagwiritsidwa ntchito, kuteteza zotupa kapena zodetsa za zitsanzo.

Makina osungunuka ofananira matrix

Mtundu wa polymer	mafa	Kukhoma Kulekerera	Kugwiritsa Ntchito Mwapadera
Polypropylene (pp)	1-12	600 bar	LC-MS ma molojekiti okhala ndi Ion-Bat Stogents (0.1% TFA)
Polythetheretherketrone (peek)	0-14	> 1000 bar	Makina a Uhplc (Acetunitrile <0.5% oposa 72h)
Kusinthika kosinthika	N / A	400 bar	Treptide Telekisis (20-30% Kukonzanso Kubwezeretsa VS Yopanda)

2. Kugwirizana pakati pa kapu ndi zipsera

Kapu ndi septaKusankhidwa ndikofunikira kuti ndifatse mipata ya 18mm. Amakhala ngati zotchinga zodetsa ndi kutulutsa, choncho ganizirani izi zisanachitike kusankha kwanu:

Mitundu ya Cap: Pali mitundu yosiyanasiyana ya zigamba yomwe ilipo, monga zipewa za stchp, zipewa zotsekera, zomwe zitha kukhala ndi zida zanu zapantchito. Kuyika mafakizerizidwe posankha imodzi yogwiritsira ntchito.

Mabuku a SePTA: Septa imamangidwa pogwiritsa ntchito zida ngati polytetrafluoro sollene (ptfe) kapena silika, motero onetsetsani kuti mumatha kugwiritsa ntchito zitsanzo zomwe mukufuna kuzigwiritsa ntchito powayesa. PTF imaperekanso kukana kwakukulu kwamankhwala pomwe ziphani silikazi zimapereka katundu wodalirika.

3. Ntchito ndi kugwiritsa ntchito milandu

Kugwiritsa ntchito ndi kugwiritsa ntchito ndi njira ziwiri zophunzitsira mukamasankha mipanda 18mm. Ganizirani:

Buku la zitsanzo: Mukadzaza mbale ndi zitsanzo zowunikira, dziwani kuti amasunga buku lanu lomwe mukufuna. Kuchulukitsa kapena kukwaniritsa kungasokoneze kulondola kwa zotsatira za chromatographic.

Njira Yowunikira: Njira zosiyanasiyana za chromatograph, monga chromatography (GC) ndi ma chromatography (LC), amafuna mitundu kapena zida kapena zida; Dziwani zomwe mukufuna kuchita musanachitike.

Mtundu wa zitsanzo: Mankhwala anu a zitsanzo ndi njira ya Adsororption imathandizira kuti zinthu ndi zitsamba zitheke mukamasankha zinthu zamtengo wapatali komanso zithandizo zapamwamba.

Zofunikira: Zingaganizire ngati ntchito yanu imafunikira chisindikizo cha airtight kuti mupewe kusinthasintha kapena kuipitsidwa.

Mukamasankha mipanda 18mm cromography, ofufuza ayenera kuganizira mosamala kugwirizana ndi zinthu zakuthupi, kapu ndi septa kusankha ndi kugwiritsa ntchito milandu yovomerezeka kuti mupeze ziweto zoyenera. Poganizira za zinthuzi, ofufuzawo amatha kuonetsetsa zoyesa zawo za chromatographic zimachitika molondola komanso kudalirika, ndikupanga zotsatira zodalirika za labu.

Onani maubwino a kugwiritsa ntchito Mbale Zam'mutu Pazomwene:Chifukwa chiyani mbale zamtchire ndizogwiritsidwa ntchito mu chromatography? 12

M'mbuyomu:

Mitengo yokwanira pamitengo ya chromatography ya malo ofufuza

Ena:

Mitengo ya chromatography ya kuwongolera kuwonetsera