Zitsanzo zamtunduwu zimakhudza kwambiri. Zimakhudza njirayi komanso zotsatira za chromatotography. Ngati vilosiyo yatsukidwa molakwika, idzaipitsa. Izi zitha kuchititsa: Mphepo ya Ghost, kuwonongeka kwachitsanzo, kusazengereza, kusanthula kwamakina, ndipo nsonga zoyambitsidwa ndi zosungunulira ndi septum. Mbale zogwirizanitsidwa molakwika zidzakhudza zotsatira zoyeserera. Izi zimapangitsa kupatuka pazotsatira komanso kuwononga autosambler.

Chifukwa chake, kuyeretsaNdulu ya Autosambirndizofunikira kwambiri. Woyesererayo ayenera kudziwa njira yoyeretsa vial.

Ndikufuna kudziwa zambiri za HPLC Vials, chonde onani nkhaniyi: Encyclopedia ya HPLC Vials

Bwanji

Njira yotsuka ndiyosiyana malinga ndi kuchuluka kwa kuipitsidwa kwa vial. Nkhaniyi iyankha njira zambiri zoyeretsa vial. Adzakuthandizani kumaliza mayeso a chromatographic.

Njira 1

1. Thirani yankho la mayesovial ya HPLC.

2. Bwerani ndi mtengo wonse mu 95% mowa, uyeretse kawiri, kenako ndikuthira madzi owuma (monyinyirika)

3. Thirani m'madzi oyera ndikuyeretsa kawiri.

4. Thirani madzi ochapira mu vial ndi kuphika madigiri 110 Celsius kwa maola 1-2 (osaphika kutentha kwakukulu, komwe kungawononge vial).

5. Zabwino komanso sitolo.

Njira 2

1.rinese kangapo ndi madzi ampopi.

2. Muzimitse mu chomera chamadzi osungunuka ndikugwiritsa ntchito zoyeretsa zomwe akupanga kwa mphindi khumi ndi zisanu.

3. Sinthani madzi ndikuupatsa mphindi khumi ndi zisanu za akupanga.

5. Pomaliza, chotsani ndi kuzilola kuti chizikhala chouma ndi mpweya.

Njira 3

1. Imbani ku Methanol kwa mphindi makumi awiri, ndiye kuti muchotse methanol ndikuyeretsa ndi ultrasonigraphy.

2. Pambuyo kuwonjezera madzi ambiri ku sampu, akupanga amayeretsa kwa mphindi 20, kenako ndikukhetsa madzi.

3. LolanivialKuuma.

Njira 4

1. Muzimutsuka ndikuwuma ndi madzi oyera poyamba, kenako zilowetsa mu womasulira wamphamvu wozizira (potaziyamu Dichromate sulfate) kuyeretsa.

2. Zilowerere kumwa mowa kwa mankhwala kwa maola opitilira 4, ndiye kuti ma usupe aukhondo kwa theka la ora, ndiye kutsanulira mowa kwa ola limodzi, ndikugwiritsa ntchito madzi akupanga kwa theka la ola, nadzatsuka ndi madzi ndi youma.

Njira 5

1. Zilowerere mu yoyeretsa yoyeza yozizira kwa maola 24.

2. Tchulani madzi okwanira 3 ndi madzi owoneka pansi pa ma acrasonic, ndipo pamapeto pake ziyeretse kamodzi ndi methanol, ndikuwumitsa kuti mugwiritse ntchito.

Njira 6

Gwiritsani ntchito kutsuka makina. Makina ochapira botolo amatha kusintha bwino zaluso zoyeretsa ndi kupulumutsa anthu ndi nthawi.

Kodi mukudziwanso momwe mungayeretse mbale zanu za HPLC? Onani nkhaniyi: Njira zisanu zoyeretsa za HPLC Vials

Mavuto omwe mungakumane nawo

Pali zovuta zambiri ndi kuyeretsa pamanja.

1. Kuyeretsa pang'ono, kutsuka nthawi yayitali, kuwononga nthawi ndi kuyesetsa. Kuphatikiza apo, kuchuluka kwa mabuluko ndi zotsekemera ndikovuta, kovuta kusamalira, ndipo zimawononga madzi.

2. Kuyeretsa kumatha kumakona akufa, kukonzanso nkovuta kutsimikizira, kukhudzana ndi zotsatirapo zoyeserera, ndikuwonjezera mphamvu zakuthupi.

3. Pali ngozi za chitetezo poyeretsa anthu oyeretsa. Makobota a laboratori azigwiritsa ntchito makina oyeretsera akupanga. Njira yoyeretsa ili imasintha bwino kuyeretsa ndi ukhondo pamlingo wina, komanso kumabweretsa zovuta.

Makina oyeretsa ndi owopsa komanso ankhanza pogwira ntchitoyo, ndipo ndikosavuta kuthyola vial, ndipo nthawi yoyeretsa sikophweka kumvetsetsa. Chifukwa chake, tikukulimbikitsani kuti muvale magolovesi, magalasi, khutu ndi zida zina zoteteza chitetezo panthawi yotsuka.

Ngati vial ndi yokalamba kapena yowonongeka, musagwiritsenso ntchito. Kutengera nthawi yomwe mumayesa, m'malo mwakevialsmiyezi 1-3 iliyonse.

Mbale zotayidwa zimayenera kulinganizidwa mofananamo, yogwiritsidwa ntchito molingana ndi malamulo osungirako zinyalala, ndipo adaperekanso makampani oyenerera oyenerera omwe amatayidwa.

Njira iliyonse yoyeretsera yomwe ili ndi maubwino ofanana ndi zovuta. Njira yabwino kwambiri ndikusintha vuli yatsopano pambuyo poyesa chilichonse. Ndikofunikira kuti musankhe njira yoyenera yoyeretsera. Kutengera mtundu wa zitsanzo zoyeserera ndi zochitika zenizeni za labotale, kusankha kumeneku kuyenera kupangidwa. Tiyeneranso kulinganiza kutaya mayesowo. Tiyenera kukumbukira kuti kutsatira mayeso onse a crobomatraphic, septum ya vial yazomwe zimafunikira kusintha.

Mukudziwa zambiri za momwe angayeretsere ma chromatography y, chonde onani nkhaniyi: Zothandiza! Njira 5 zoyeretsa ma chromatography

M'mbuyomu:

Sankhani Mbale Zoyenera HPLC kuti mukonzetse kusanthula kwanu kwa HPLC

Ena:

Kodi mukumvetsa kuwongolera kwamiyendo ya HPLC?