Ungasarudza sei iyo yakakodzera HPLC VID septa yeSolvent yako?

Yepamusoro performance yemvura chromatography (HPLC) ndiyo yakakosha imbudzi nzira inoshandiswa muminda dzakasiyana-siyana senge mishonga, nekuchengetedzwa kwezvakatipoteredza, uye kuchengetedzwa kwekudya. Chikamu chakakosha cheHPLC system ndeye vial septum, iyo inoshanda sekuvhara kweiyo vial. Chidimbu cheSeptum ndeyevhavha inosimbisa iyo vial kuvhura, kubvumira sindonji yekupeni uku uchiramba uine chisimbiso chakachengeteka kudzivirira kusvibiswa uye kubuda kwemvura. Ivo vanofanirwa kuwirirana neiyo solvent kuongororwa uye inofanira kumira muHPLC maitiro emamiriro ezvinhu.

Kukosha kwekusarudza iyo ScASt septa

Kusarudza septum chaiyo kwakakosha ku:

Kudzivirira Kusvibiswa: Zvinhu zvisingawirirane zvinogona kuona mumuenzaniso kana kuita neiyo solvent.

Kuverengera chisimbiso chakakodzera: Chisimbiso chakanaka chinodzivirira kurasikirwa kwemuenzaniso uye kuchengeta kutendeseka.

Kufambisa majekiseni mazhinji: Zvimwe Septa zvakagadzirirwa kushandisazve pasina kudzvanywa.

Unoda kuziva ruzivo rwakazara nezvePTFE \ / Silicone Septa, ndapota tarisa chinyorwa ichi: Zvese zvaunoda kuziva: 137 Pre-Slit PTFE \ / Silicone Septa facs

HPLC VID septa zvinhu mhando

Zvishandiso zvakasiyana zvine zvivakwa zvakasiyana zvinokanganisa kuita kwavo muHPLC application. Izvi zvinotevera zvinhu zvinowanzoshandiswa muHPLC VIDS septa:

1. PTFE (PolytetraFluoroetheryle)

Zvimiro: PTFE ine yakanaka yemakemikari kuramba, iner inert uye inogona kutsungirira zvine simba sol.

Inoshandisa: isina kukodzera kujekesa kwakawanda kana kureba-kwenguva yakareba nekuda kwekutadza kwayo mushure mekutakurwa, zvichiita kuti ive yakakodzera kushandiswa kweimwe-kushandisa.

Yakanyanya kupisa kudzikisira: Mazano ekupisa anosvika 260 ° C.

Mutengo: Kazhinji hupfumi hunofananidzwa nezvimwe zvinhu.

2. PTFE \ / Silicone Septa

Zvimiro: Inosanganisa kumakemikari kurwisa PTFE nekutsvagirwa kweSilicone.

Inoshandisa: Yakakurudzirwa majekiseni akawanda uye sampula yekuchengetedza nekuda kwekutsvagirwa kwayo kwakanaka.

Tembiricha yeTembiricha: inoshanda kubva -40 ° C kusvika 200 ° C.

Autoclavable: Inokodzera maitiro ekushungurudza.

3. Pre-Cheka PTFE \ / Silicone Septa

Zvimiro: Yakagadzirirwa kuderedza kuora kana uchishandisa masango akajeka-akaiswa pasi; inoderedza macuum fomu mumakamuri.

Inoshandisa: Inokodzera kuti application ipo nguva dzose sindonle yekuonana inodikanwa pasina kukanganisa kweSepum Sept.

Tembiricha Range: Zvakafanana nePTFE \ / Silicone, -40 ° C kusvika 200 ° C.

4. Butyl Rubber

Zvivakwa: Zvakanaka kuisimbisa zvivakwa, asi kushoma kwemakemikari kuramba zvichienzaniswa nePTFE.

Inoshandisa: Inokodzera mhinduro dzemvura uye kushoma solhives; inogona kushandiswa mune zvekushandisa inoda chisimbiso chemahara.

Kukwikwidza kwakasimba kwekushisa: zvakanaka pamadzinza ekudziya, asi zvinogona kusvibisa pakupisa kwakanyanya kana nehasha sol.

5. Viton

Zvivakwa: Kudzivirira zvakanyanya kumakemikari ane hutsinye uye anochengetedza mamwe kugona kusevha mushure mekuputika.

Inoshandisa: inotariswa kwekupedzisira kusimudzira nekuda kwemutengo wakanyanya; Inokodzera kune zvinoshandiswa kuti zvimwe zvinhu zvakundikana.

Kuwedzera kupisa kwekushomeka: zvinobudirira pakudziya kwakanyanya, asi zvinoda kubata nekuchenjera nekuda kwekuomarara kwayo.

Zvinhu zvekufunga kana uchisarudza HPLC VIDS seka

1. Kemical kuwirirana

Chinhu chekutanga kufunga nezvemakemikari kuwirirana kweSeptum zvinhu pamwe nemari yaunoronga kushandisa. Zvishandiso zvakasiyana zvine zvirevo zvakasiyana kumakemikari:

PTFE (PolytetraFluoroHeryle): PTFE inozivikanwa nekuda kwekuramba kwayo kwemakemikari uye yakakodzera kune yakasarudzika mhando shoma, kusanganisira makemikari anodzora. Inowanzoshandiswa mukubatanidzwa nezvimwe zvinhu.

Silicone: Silicone Septa inoshanduka uye inogona kuwedzerwa shure mushure mekupinzwa, kuita kuti vakwanise kuwanika majekiseni. Nekudaro, ivo vanogona kunge vasingaenderane nemasimba ese.

Butyl Rubber: Izvi zvinyorwa zvine yakanaka kuiswa midziyo yezvivakwa, asi ine mashoma emakemikari kuramba kuenzaniswa nePTFE.

PTFE \ / SILICONE Composite: Inosanganisa zvakanakira zvinhu zvese zviri zviviri, zvine makemikari akanaka emakemikari uye atsvage.

2. Solvent mhando

Rudzi rweSolvent yakashandiswa zvakare ichapesvedzera Septum kusarudzwa:

Mhinduro dzemvura: Kune mvura-yakavakirwa sol sol, silicone kana ptfe \ / silicone secsa inowanzo kukurudzirwa nekuda kwekuenderana kwavo kwakanyanya.

Organic solventes: ye organsic solhanol senge methanol kana acetonitrile, ptfe kana ptfe \ / / sticone makompositendi inodiwa nekuda kwekurwisa kwemakemikari avo.

Corrosive makemikari: Kune inokatyamadza sol solies yakadai sosi yakasimba kana zvigadziko, ptfe kazhinji ndiyo sarudzo yakanyanya kunaka.

3. Kuita basa

Kugona kweSeptum kuti uwedzere mushure meganda retsono kwakakosha kuchengetedza kutendeka kwemuenzaniso:

Slit vs. Yakasarudzika Dhizaini: Zvimwe Septa zvine masheya kuti afambe nehosha, asi izvi zvinogona kukanganisa chisimbiso nekufamba kwenguva. Isiri-slit dhizaini inopa chisimbiso chakasimba asi chingangoda simba rakawanda kana kujekesa.

Shongwe: Tarisa seSchca inogona kuwanzokwana kubereka mushure mekuwanda kwetsika dzakawanda kuti udzivise kusvibiswa uye kubuda kwemvura.

Unoda kuziva kuti kusarudza Septa muSt-Slit kana Kwete? Ndokumbira utarise chinyorwa ichi: Ungasarudza sei Septa pre-slit kana kwete?

4. Kunobviswa uye mabwe

Paunenge uchishanda nema samples inomarara, zvakakosha kufunga nezve zvinowanikwa uye zvinoputika muSeptum zvinhu:

Yakadzika inogadziriswa: Sarudza Septa ine yakaderera inobvisa kuderedza njodzi yekusvibiswa. PTFE ine yakaderera yekubvisa ichienzaniswa nemidziyo yerabha-yakavakirwa.

Kuedza Zviyero: Vamwe vagadziri vanopa data pane zvinobviswa; Wongorora izvi zvirevo kana uchisarudza septum.

5. Kupisa kwakanyanya kupisa

Funga nezve tembiricha tembiricha umo yako HPLC system ichashanda:

Tembiricha yakakwira: Kana chikumbiro chako chichibatanidza tembiricha yakakwira, ita chokwadi chekuti septum zvinhu zvinogona kutsungirira mamiriro aya pasina kudzvanywa.

Cryogenic Zvikumbiro: Nekuti Cryogenic Zvikumbiro, sarudza zvinhu zvinochengetedza kutendeseka kune yakadzika tembiricha.

6. Zvivakwa zvemuviri

Maitiro epanyama eSeptum anogona zvakare kukanganisa kuita:

Bickness: Thickker Septa inogona kupa chisimbiso chiri nani asi chingangoda simba rakawanda panguva dzenjere.

Kuomesa: SFFTER ZVINHU ZVINOGONESA ZVINOGONA KUTI VATSVIRE BUDALBILUGLUBLE BUTE KUTI UVHURE KUTI URI KUTI URI KUTI URI KUTI URI KUTI URI KUDZIDZA KUSHANDISA KUSHANDISA.

Kusarudza kodzero yeHPLC vial vial inoda kunyatsofungisisa kweiyo solvent kuwirirana, kusimbisa kugona, kusimba kwakanyanya kuramba, uye zvido chaizvo zvinodiwa. Nokunzwisisa zvivakwa zvezvinhu zvakasiyana uye zvavanoshandisa, vatsvagiri zvinogona kuvandudza zvakanyanya kukosha uye kuvimbika kweHplc kuongorora. Chisarudzo chakaringana chisingapizve zvinoitwa, asi zvakare chinoderedza njodzi dzinoenderana nekurovedza muviri uye kusvibiswa.