Chromatography inzira yakakosha mune ongororo yekuongorora iyo inoshandiswa kupatsanura nekuchenesa makomputa kubva musanganiswa. Pakati penzira dzakasiyana siyana dziripo, flash chromatography uye hombe performance liquid chromatography (HPLC) nzira mbiri dzakakurumbira, imwe neimwe nezvibatsiro zvakasiyana uye zvinoshandiswa. Chinyorwa ichi chinotora zvakadzika mumitemo, zvakanakira, zvakashata, zvinoshandiswa, uye zvakazara kushanda kweFlash chromatography vs. Hplc.

Unoda kuziva zvakawanda nezveHPLC Sampula, ndapota tarisa chinyorwa ichi:HPLC yemuenzaniso yekugadzirira mhinduro dzemhedzisiro yakanaka

1. Nheyo yekushanda

Flash chromatography maitiro ekugadzirira anoshandisa makatsikiswa solinen kupfuudza sampuro kuburikidza nekhimara yakazadzwa nechikamu chamasita, kazhinji silika gel. Muenzaniso wakaremerwa mukoramu, uye sezvo iyo solvent inoyerera kuburikidza, izvo zvakasiyana zvemusanganiswa zvinoparadzaniswa zvichienderana nekudyidzana kwavo neiyo nzvimbo yekutandarira. Maitiro acho anokurumidza kutsanya, aine makomisheni achinatswa mumaminitsi mashoma.

HPLC, kune rumwe rutivi, inzira yakaoma inosanganisirawo kupfuudza sampuli yemvura kuburikidza nekhoramu yakazadzwa neyechikamu chechitima. Nekudaro, HPLC inoshanda kune yakakwirira dzvinyiriro (kusvika 4000 PSI kana more) uye kazhinji inoshandisa zvidiki zvidiki zvimiti zvikamu (3-5 μM). Izvi zvinobvumidza kupatsanurwa kwepamusoro uye kugadzirisa pane kupenya chromatography. Nekuti Hplc inogona kunyatso kudzora tembiricha, kumanikidzwa, uye mareti ekuyerera, inogona kuwana zvakanyatso kuparadzaniswa.

2. Zvishandiso uye Setup

Midziyo yehunyanzvi mbiri dzakasiyana kwazvo:

Flash chromatography inoda iyo yakapfava setup inosanganisira chipfuyo chechikamu uye pombi yekuendesa iyo nhare chikamu. Iyo inowanzowedzera mutengo-inoshanda uye nyore nyore kushanda pane iyo HPLC system.

HPLC Systems yakawedzera kuoma, ine mapapipi akasarudzika, anoongorora (seUV-vis-vis), uye nedatha data yekuwana data. Nekuti Hplc zvishandiso zvinonongedzowo tekinoroji zvakanyorwa, yekutanga investment inowanzo kukwirira.

3. Kumhanya uye kushanda zvakanaka

Imwe yemisiyano yakakosha pakati peiyo flash chromatography uye Hplc ndiyo kumhanya kwavo:

Flash chromatography inogona kuchenesa makomputa nekukurumidza, kazhinji kazhinji kupedzisa kupatsanurana mukati memaminitsi makumi matatu. Uku kumhanya kwakanyatsoshanda kune organic makemikist anofanirwa kukurumidza kupatsanura maitiro ezvigadzirwa.

Nepo Hplc yakanyatsobudirira maererano nehunhu hwekuparadzanisa, zvinowanzotora nguva yakareba kuti upedze mabasa akafanana neakasarudzika uye Simba rezvemhando yepamusoro - imwe neimwe yemuenzaniso inomhanya inowanzo kutora anopfuura awa.

4. Kuchena uye kugadzirisa

Flash chromatography inoshanda kwazvo kune yepakati yakanatswa kucheneswa, asi inogona kusapa huwandu hwakanyanya hwekuchena hunodiwa pakupedzisira kuongororwa kwechigadzirwa. Zvinogona kazhinji kuwana zviyero zvakanaka zvekuchena, asi zvinogona kunge zvisina kukodzera kusanganisa kwakaoma kwazvo uko kusarudzika kwekugadziriswa kwakakosha.

Hplc inodarika mukupa yakanyanya kuchena makomputa nekuda kwekugona kwayo kune zvakaringana tune kupatsanurana mamiriro ezvinhu. Iyo kazhinji ndiyo nzira yesarudzo kana uchibata nemusanganiswa wakaomarara kana kana pakakwirira yekuparadzanisa yekuparadzanisa inodiwa.

5. SAMPLE SANGANO

Flash chromatography inowanzotendera mhando yakakura yemuenzaniso kupfuura HPLC. Kugona uku kunoita kuti zviite kuti zvinyatso kuvezwa huwandu hwakawanda hwemakomputa, izvo zvinobatsira panguva yekutanga synthesis matanho.

HPLC, apo inokwanisa kubata huwandu hwakawanda hwemhando yemuenzaniso, kazhinji ine yakaderera mutoro masangano nekuda kweiyo diki diki yekisimusi chikamu uye yakakwirira kumanikidza zvinodiwa.

6. Kufunga nezve

Mutengo chinhu chakakosha pavanosarudza pakati peFlash chromatography uye Hplc:

Flash chromatography haina kudhura, zvese maererano nemidziyo uye mari inoshanda. Zviri nyore kumisikidza uye inoda kunyatsogadziriswa, ichiita kuti iite kuti iite marebarati ane mabhajeti mashoma.

Kunyangwe HPLC ine sarudzo yakanaka uye yekuchena, ine mutengo wepamusoro wekushanda nekuda kwekunetseka kwehurongwa uye kukosha kwekudhura kunodhura kwakadaro semakoramu uye sol sol.

7. Zvikumbiro

Matanho ese ari maviri ane zvakasiyana kushandiswa muminda dzakasiyana-siyana:

Flash chromatography inoshandiswa zvakanyanya mu organic synthesis yekukurumidza kunatswa kwezvigadzirwa zvekuita. Izvo zvinonyanya kufarirwa munzvimbo dzedzidzo uko kukurumidza kupatsanurana kwemakomputa kunodiwa mumabasa ekutsvagisa.

HPLC inoshandiswa zvakanyanya mumunda wemishonga yekuongorora kwezvinodhiriso, kuwanikwa kwekuedza kwezvakatipoteredza, zvinosvibisa mukuchengetedzeka kwekuchengetedza, uye biochemical zvidzidzo zvinoongororwa zvakaringana zvekuongorora zvinodiwa.

Zvakanakira uye Zvakashata

Flash chromatography

Zvakanakira: Nguva yekukurumidza kunatswa. Kuderera zvishandiso uye mari yekushandisa. Simpler setup uye kushoma hunyanzvi hunyanzvi hunodiwa. Sampu yepamusoro inotakura mukana.

Zvinokuvadza: Desiran Resolution tichienzaniswa neHPLC. Inogona kunge isiri kuzadzisa iyo yakanyanya yekuchena mazinga anodikanwa ekupedzisira chigadzirwa kuongororwa. Zvishoma kushanda kwemasanganiswa akaoma mashoma ayo anoda kupatsanurwa kwakanaka.

Yakakwira performance liquid chromatography

Zvakanakira: Kugadziriswa kwakakwirira uye huwandu hwekuchena. Kukwanisa kunyatsoongorora kusagadzikana kusanganiswa. Chaizvo kutonga kwemamiriro ekushanda anovandudza kubereka.

Zvinokuvadza: zvishoma nezvishoma nguva yekugadzirisa. Yakakwirira yekutanga investment uye mari inoshanda. Midziyo yakaoma kunzwisisa inoda kudzidziswa kwakasarudzika.

Mune pfupiso, Flash chromatography uye HPLC imwe neimwe ine zvakanakira uye zvakashata uye zvakakodzera kuti zvibodzwa zvakasiyana mukuongororwa kweMuongorori. Flash chromatography nekukurumidza uye yakaderera-mutengo, ichiita kuti ive yakakodzera yekukurumidza kunatswa kwebasa mu orghic synthesis. Kusiyana neizvi

Unoda kuziva mhinduro makumi mashanu nezve HPLC vials, ndapota tarisa chinyorwa ichi:50 Mibvunzo inowanzo bvunzwa paHPLC vials

Pakupedzisira, sarudzo pakati peFlash chromatography uye HPLC inoda pazvinhu zvakananga zvekutarha Kunzwisisa misiyano iyi inobvumira vaongorori kuti vasarudze tekinoroji yakakodzera kwazvo kune avo zvavanoda pachavo, vachisimbisa zvakanakira mhedzisiro ye chromatographic ongorora.

Yapfuura:

Ungasarudza sei iyo yakakodzera HPLC VID septa yeSolvent yako?

Hupplc yakasiyana sei neGC Column?