Hupplc yakasiyana sei neGC Column?

HPLC (Yakakwira Performance Liquid Chromatography) uye GC (Gasi Chromatography) Ndege dziri mbiri dzekuongorora dzinoshandiswa kuparadzanisa, zivisa, uye dzinokosha makomputa mumhando dzakasiyana siyana. Nekudaro, ivo vanosiyana zvakanyanya maererano nekuvhiya, zvishandiso, uye zvinoshandiswa. Chinyorwa ichi chinoburitsa mutsauko wakakosha pakati peHPLC uye GC makoramu, kutarisa pane yavo dhizaini, kushanda, uye kukodzera kwemhando dzakasiyana dzekuongorora.

Column dhizaini

HPLC makoramu

HPLC makoramu anowanzo kupfupika uye akafara kupfuura gc columns. Ivo vanowanzoita kusvika pamakumi matatu emamita pakureba uye vane madhiri emukati kubva ku2,1 mm kusvika 8 mm. Kurongedza mukati meHPLC makoramu kune zvidiki zvidiki zvimedu (zvinowanzoita zvisingasviki mashanu microns mune diameter) iyo inopa nzvimbo yakakura yekubatanidza nezvikamu zve samples. Iko kurongedza zvivakwa zvemakoramu aya zvinovabvumidza kuti zviite zvakanaka kuzvitsaurira makomputa anoenderana nezvivakwa zvavo zvemakemikari.

Main Main:

Kureba: kusvika pamakumi matatu cm

DIAMETER: Kazhinji pakati pe2,1 mm uye 8 mm

Packagation midziyo: zvidiki zvidimbu

GC makoramu

GC Mbiru, nekusiyana, ndezvekusiyana uye marefu uye madiki, kazhinji anosvika zana emamita uye ana dhivha yemukati kubva ku0.1 mm kusvika 1 mm. Ivo vanogona kukamurwa kuva marudzi maviri makuru: akazara makoramu uye capillary makoramu. Akarongedzwa makoramu ane chikamu chakasimba chechikamu kana kuti mvura yakabatanidzwa nerutsigiro rwakasimba, nepo capillary makoramu ane firimu rakatetepa rekiredhiti yemukati yakaunganidzwa pamadziro emukati.

Main Main:

Kureba: kusvika zana m

DIAMETER: Kazhinji pakati pe1.1 mm uye 1 mm

Mhando: Yakarongedzwa makoramu (yakasimba kana yemvura station

Nhare mbozha

Yakakwira performance liquid chromatography

MuHPLC, iyo nhare chikamu inowanzo solvent solvent kana musanganiswa we polar kana isiri-polar sol. Solid solses inosanganisira mvura, methanol, acetonitrile, uye akasiyana-siyana buffers. Sarudzo yefoni nhare inokoshesa nekuti inokanganisa kudyidzana pakati peiyo ana ana analyte uye chikamu chechiteshi mukati mekamuri.

Gasi chromatography

GC inoshandisa gakisi yegirazi nhare, kazhinji inert gasi senge helium kana nitrogen. Muenzaniso unofanira kuve wakaoma zvakakwana kuti udzokere kana ukaziviswa mukoramu. Ichi chinodiwa chinoreva kuti GC inonyanya kuongorora kuongorora mishonga inosiririsa, nepo Hplc inogona kubata huwandu hwakakura hwezvinhu, kusanganisira zvisingaiti macomputer.

Kuparadzanisa Mechanism

Yakakwira performance liquid chromatography

HPLL inoparadzanisa makomputa zvichibva pakubatana kwavo kweiyo nzvimbo yekutandarira yekisimusi yedanho refoni. Maitiro akasiyana-siyana e chromatography anogona kushandiswa:

Yakatenderwa Phase Chromatography: Nonpolar Stateary chikamu chine polar mobile chikamu.

Zvakajairika chikamu chromatography: polar stationary chikamu chine nonpotlar mobile chikamu.

Ion exchangeraphy: inoparadzanisa yakarongedzwa mhando zvichienderana nekudyidzana kwavo neiyo yakabhadharwa stationary chikamu.

Saizi yekubatanidza chromatography: inoparadzanisa mamorekuru zvichienderana nehukuru.

Gasi chromatography

Mune gasi chromatography, kuparadzaniswa inonyanya kuwanikwa nemisiyano mukushamisika uye inovhara pfungwa dzekuongorora. Compound inobuda nyore nyore kuda kubva kuColumn kutanga, nepo isinganyanye mishonga isina kujairika ichatora nguva yakareba ichapfuura. Kubatanidzwa pakati pemufundisi uye chikamu chechiteshi chinogona zvakare kukanganisa nguva yekuchengeta nguva.

Kunzwa uye kugadzirisa

HPLC Sensitivity

HPLC kazhinji ine yakakwirira kunzwa kune isiri-isina-isina kujairika makomputa nekuti inokwanisa kuongorora zvakadzika makomendi emasampuli asina kubuda. Uchishandisa zvidiki zvidiki zvechikamu muHPLC makoramu anopa nzvimbo yakakura yekudyidzana, iyo inovandudza resolution.

GC Sensitivity

Sezvo gasi chromatography inokwanisa kuisa pfungwa yekuongorora kuburikidza nekuvapura, inokwanisa kuwana yakanyanya kunzwa kune mishonga isina kujairika. Capillary makoramu anowanzo kuve nekugadziriswa kuri nani pane yakarongedzwa columns nekuda kwekureba kwavo kwenguva refu uye diki diki.

Zvikumbiro zveHPLC uye GC

HPLC zvikumbiro

HPLC inoshandiswa mune dzakasiyana siyana minda nekuda kwekushandiswa kwayo.

Kuongororwa kwechechi: inoshandiswa kune zvinodhaka kuongororwa uye kudzora kwemhando.

Kuedzwa kwezvakatipoteredza: Ongorora zvinosvibisa mumvura uye ivhu samples.

Chikafu Chekuchengetedza Kwekudzokorora: ona zvinosvibisa uye simbisa hunhu.

Biotechnology: chinatsa mapuroteni uye nucleic acids.

Gasi chromatography application

GC inonyanya kushandiswa kuongorora zvisingaiti organic makomputa:

Kuongorora kwezvakatipoteredza: Kuyera Vakaenzana Organic Compounds muMhepo Pollutants uye Mvura.

Maonero esainzi: kuongorora zvinhu pazvinhu zvematsotsi.

Indasitiri yePetrochemical: inoratidza hydrocarbons mufuti.

Flavour uye Anonhuhwirira kuongorora: Kuzivisa zvinhu zvakaoma mune zvekudya.

Mukupfupikisa, HPLC uye GC dzakasiyana chromatographic marudzi akasiyana emhando dzakasiyana dzekuongororwa zvichibva pane yavo yekugadzira, chikamu chekuparadzanisa, chikumbiro, kunzwa, uye kushushikana kwekugadzirisa. HPLL yakakodzera isina-kusiririsa kana iyo lible Kunzwisisa misiyano iyi inobvumira vaongorori kuti vasarudze nzira yakakodzera kune yavo chaiyo yekuongorora zvinodiwa, ichiita kuti migumisiro yakaringana muminda yakasiyana siyana yesainzi.

Iwe unoziva mutsauko pakati peHPLC vials uye gc vials? Tarisa chinyorwa ichi: Ndeupi musiyano uripo pakati peHPLC vials uye gc vials?