Sep. 14th, 2024
Gasi Chromatography-Mass SpectRogry (GC-MS) uye Liquid chromatography-ms Nzira mbiri idzi dzakagadzirirwa kupatsanura uye kuona makomputa mumisanganiswa yakaoma, asi nheyo dzadzo dzekubata, zvikumbiro, uye zvakanakira zvakasiyana. Chinyorwa ichi chinoongorora misiyano pakati peGC-ms uye LC-MS kubatsira vaongorori vanoona kuti ndeipi nzira yakanakira kune zvavanoda chaizvo.
GC-MS uye LC-MS Overview
Gasi chromatography-misa spectrometry (GC-MS)
GC-MS inosanganisa gasi chromatography uye misa misa yekuongorora kuongorora zvisikwa uye semipolatile componeunds. Mune iyi nzira, sampuli inovharirwa uye inotakurwa kuburikidza ne chromatographic column neine syrer gasi, kazhinji helium. Iyo gasi chromatography component inopatsanurana makomputa kubva pane yavo kujairika uye kudyidzana necolumn stationary phase. Mushure mekuparadzaniswa, ma compounds anounzwa mumasere ekutarisa idzo dzavari iionised uye yavo yakawanda-ku-kubhadharisa chiyero chiyero chinoyerwa. Maitiro aya anogona kuziva uye kujekesa makomputa aripo mumuenzaniso.
Zvikumbiro zveGC-MS:
Kuongorora Kwekufunga: GC-MS ndiyo goridhe madhiragi ekuzivisa zvinodhaka uye zvigunwe muzvikamu zvebhaibheri.
Kuongorora zvakatipoteredza: Inoshandiswa kuona zvisina kunaka uye zvinhu zvine ngozi mumhepo, mvura, uye ivhu.
Kuchengetedza Kwekudya: GC-MS inogona kuziva zvipembenene zvakasara uye zvimwe zvinosvibisa mune zvigadzirwa zvekudya.
Mvura chromatography-misa spectrometry (lc-ms)
LC-MS inobatanidza Liquid chromatography ine misa spectrometry, ichiita kuti ive yakakodzera kuongorora huwandu hwakawanda hwemakomputa, kusanganisira izvo izvo zvinokanganisa kana zvisina kujairika. MuLC-MS, sampuli inovharwa muchikamu chemvura chinwiwa, icho chinoputirwa kuburikidza nekhoramu yakarongedzwa nechikamu chenzvimbo. Iwo ma compounds akapatsanurwa zvichienderana nezvivakwa zvavo zvemakemikari, uye mushure mekuparadzaniswa, ivo vane ionised uye kuongororwa neyekuongorora mashure.
Zvikumbiro zveLC-ms:
Kutsvaga kweMishonga: LC-MS INONYANYA KUTI URI KUTI URI KUTI URI KUTI URI KUTI URI KUTI URI KUTI URI KUTI URI KUTI URI KUTI URI KUTI URI KUTI URI KUTI URI KUDZIDZA.
Biotechnology: Izvo zvakakosha kuti uongorore mapuroteni, mapepeti, uye acclic acids.
Clinical diagnostics: LC-ms inoshandirwa mukuongororwa kwevakadzi vebhaibheri uye zvinodhaka zvinodhaka mumirauro yemiramu.
Kuongororwa kwezvakatipoteredza: Zvakafanana neGC-MS, LC-ms inoshandiswa kuona zvinosvibisa mumatanho akasiyana-siyana, kusanganisira mvura uye ivhu.
Unoda kuziva zvakawanda nezvezvinoshandiswa zve chromatography vials, ndapota tarisa chinyorwa ichi: 15 zvikumbiro zve chromatography vials muminda dzakasiyana
Misoro yakakosha pakati peGC-MS uye LC-MS
1. Nhare
Musiyano wakanyanya kukosha pakati peGC-ms uye LC-MS ndiyo nhare chikamu chinoshandiswa kuparadzaniswa. GC-MS Inoshandisa iyo Gasi Dhifeni chikamu, ichiita kuti ive yakakodzera kune isina kujairika uye hafu-isina mishonga. Mukupesana, LC-MS inoshandisa iyo Liquid Mobile chikamu, ichibvumira kuongororwa kwemhando dzakasiyana-siyana dzemakomputa, kusanganisira izvo zvisingagone kupererwa pasina kudzvanywa.
2. Kuenzanisa Kugadzirira uye Kuenderana
GC-MS inoda samples kuve mune isiri-polar solvent uye inofanira kuve yakarongedzwa pamberi pekuongororwa. Ichi chinodiwa chinokonzerwa nekushanda kwayo kune makomputa neakadzika mapoinzi ekudzikira uye kugadzikana kwekutonga. Kukurukurirana, LC-ms inogona kuongorora samples muPolar soles uye inoenderana neyakaomarara biology matrices, ichiita kuti iite yakakodzera huwandu hwekuongorora, kusanganisira biobollecules.
3. Kunzwa uye kuona miganhu
Matipi ese ari maviri anopa zvakanyanya kunzwa, asi kuita kwavo kunogona kusiyana zvichienderana neyekuongorora. GC-ms inowanzove inotarisisa mishonga isina kujairika, nepo LC-ms ine sensitivity yekunzwisisa isiri-isina kujairika uye inopisa LC-ms inogona zvakare kuwana yakaderera kutarisa miganhu yemamwe makirasi emakomputa, senge mishonga yemishonga uye biobolecules.
4. Mari inoshanda uye kuoma
GC -MS System inowanzo kuve isina kuoma uye inoda kushoma nyanzvi yekudzidziswa kupfuura LC-ms masisitimu. Semhedzisiro, GC-ms inogona kuve yakawanda mutengo-inoshanda kune marebarendi ne bhajeti yekumanikidza. LC-ms, ichiratidze simba rekushandisa, inosanganisira zvimwe zvikamu uye kugadzirisa, izvo zvinogona kuwedzera mari yekushanda.
Kusarudza nzira yekurudyi yekutsvaga kwako
Kana uchisarudza pakati peGC-ms uye LC-MS, vatsvagiri vanofanirwa kufunga nezvezvikamu zvakati:
Chimiro chemaongorori: Kana iyo target compounds is islashile uye inotsvedza yakagadzikana, GC-MS inogona kunge iri sarudzo yaunoda. Zvekukura, zvisiri-zvisina simba, kana kuti zvinokatyamadza zvisingaenzaniswi, LC-MS zvine kukodzera.
SAMPLE MATRIX: kuoma kweiyo sampuli matrix kunogona kukanganisa sarudzo yehunyanzvi. LC-ms inowanzo kuve nani kune biological samples, nepo GC-ms inodarika mukushandisa kwezvakatipoteredza uye maficha.
Sensitivity Zvinodiwa: Kana iyo yekutsvaga ichida kuwonekwa kwepasi pekuvhara kweasina-kusiririsa makomputa, LC-MS inogona kupa iyo inodiwa kunzwa kwekunzwa.
Budget uye zviwanikwa: Funga nezve mari yekushanda, kusanganisira kuchengetedza uye kudzidziswa, kana uchisarudza maitiro. GC-MS inogona kuve yakajeka zvakanyanya kune madiki madiki madiki ane mashoma zviwanikwa.
Unoda kuziva mhinduro makumi mashanu nezve HPLC vials, ndapota tarisa chinyorwa ichi: 50 Mibvunzo inowanzo bvunzwa paHPLC vials
Mhedziso
Zvese gc-ms uye lc-ms ine hunyanzvi hwekuongorora maitiro nezvakanaka zvakasiyana uye zvinoshandiswa. GC-MS yakanakira kuongorora mishonga inoshandiswa uye inoshandiswa zvakanyanya mukusurindira uye neyakatipoteredza kuongorora. Mukupesana, LC-MS ine yakasarudzika yekushanda uye inovhara mishonga uye inokonzeresa mishonga, ichiita kuti ive yakakosha mumishonga yemishonga uye yekutsvaga kweiotechnology. Pakupedzisira, sarudzo pakati peGC-ms uye LC-MS-MS inofanirwa kuve yakavakirwa pane izvo zvinodiwa zvekudzidza, kusanganisira chimiro chemaongorori, kuomarara kwemhando, uye zviwanikwa zviripo. Nekunyatsofunga nezvezvinhu izvi, vaongorori vanogona kusarudza nzira dzakakodzera kwazvo kuti dziwane zvakaringana uye zvakavimbika mhedzisiro mukuongororwa kwavo.
Chirungu
ChiChinese
