Kugadzirira kweMuenzaniso inhanho inokosha mune yemvura chromatography-misa spectrometry (lc-ms) maitiro, kukurudzira chokwadi uye kuvimbika kwemhedzisiro yekuongorora. Chinangwa chikuru chekugadzirira kwekutanga ndechekubvisa, chinatsa, uye kuisa pfungwa kuongorora kubva kumatiki akaomarara, kuve nechokwadi chekuti vanoenderana neLC-ms kuongorora. Iyi nhanho ndeyekutsoropodza kubvisa zvingangoitika zvinogona kukanganisa zvinogona kukanganisa data. Kugadzirira kwemuenzaniso kwakakodzera kunovandudza pfungwa uye kuongororwa kwekuongororwa, izvo zvakakosha kuti zvibirirwe munzvimbo dzakasiyana-siyana sepurotomics, metabolomics, mishonga yekuongorora, uye kuongororwa kwezvakatipoteredza.

Zvinangwa zvakakosha zveGemu Kuenzanisira:

Kubviswa kwekupindira: Bvisa zvinhu zvinogona kukanganisa nekuonekwa kweanenge kuongorora.

Kuongorora kwekuongorora: Wedzera kusunganidzwa kwemuongorori kuti uvandudze kuongororwa miganhu.

Kuenderana: Gadzirira samples nenzira yekuti vanogona kunyatsoongororwa zvakabudirira neLC-ms masisitimu.

Unoda kuziva zvakawanda nezveHPLC Sampula, ndapota tarisa chinyorwa ichi: HPLC yemuenzaniso yekugadzirira mhinduro dzemhedzisiro yakanaka

Maitiro Akafungwa muLC-MS Sampule Kugadzirira

Maitiro akati wandei anowanzo shandiswa muLC-MS Sampule Kugadzirira. Imwe neimwe nzira ine zvayo zvakanakira uye inosarudzwa zvichibva pane izvo zvinodikanwa zvekuongorora.

1. Solid phase yekubvisa (SPE)

SPE inoshandiswa zvakanyanya nekuzvitsaura kuongorora kubva kumasampuli emvura. Iyi nzira inosanganisira kupfuudza sampuro kuburikidza nechikamu chakasimba chechikamu icho chinoshanda chengetedza iyo inoongorora iyo yekuongorora ichitendera zvimwe zvikamu kuti zvipfuure. SPE inonyanya kushanda kune yakaoma matrices yakadai seropa kana urini uye inopa mibairo yakati:

Yakakura Kudzorera mitengo: SPE kazhinji inopa yakakwira yekudzora mari inoenzaniswa nemvura-yemvura kubviswa.

Zvekuenzanisa

2. Protein Precipitation

Protein Spiritation ndiyo nzira iri nyore asi inoshanda yekubvisa mapuroteni kubva kune biological samples. Iyi nzira inonyanya kukosha kana ichiongorora samples ine yakakwirira mapuroteni zvemukati, yakadai seplasma kana serum. Nekupururudza kwepuroteni, iyi nzira inorerutsa matrix uye inoderedza kupindira mune inotevera kuongorora.

Kurumidza uye kunoshanda: Inotendera kukurumidza kugadzirisa kwemasampula uchiramba uine hutano hwakanaka.

3. Liquid-mvura yekubvisa (lle)

Lle akapatsanura analys zvichibva pane yavo solubility mune zviwi zviviri zvisingaite - kazhinji chikamu chechikamu uye organic solvent. Iyi nzira inobatsira kubvisa isiri-polar kana zvine mwero polar compones kubva kuomarara matanho emadzinza.

Kuverengera Kushandisa: LLLE inogona kugadziridzwa kune dzakasiyana siyana sampues uye mhando, zvichiita kuti zviite zvakakodzera kuti zviwedzere huwandu hwekushandisa.

4. Derivatization

Derivatization inosanganisira yemakemari inogadzirisa ongororo yekuwedzera yavo yekuteedzera muLC-ms kuongorora. Maitiro aya anovandudza zvivakwa zvakadai sekunyanyisa uye hunhu hwekubudirira, zvichiita kuti zvive zvinoshanda zvakanyanya kune makomputa neakadzika midziyo yekuwoneka.

Yakavandudzwa kunzwa: derivatization inogona kuwedzera zvakanyanya kunzwa uye kusarudzika kwekuongorora kwemakirasi akanyorova senge amino acids kana steroids.

Unoda kuziva zvakawanda nezvezvinoshandiswa zve chromatography vials, ndapota tarisa chinyorwa ichi: 15 zvikumbiro zve chromatography vials muminda dzakasiyana

Zvinhu zvinokanganisa muenzaniso wekugadzirira nzira yekusarudzwa

Kusarudza iyo yakakodzera sampu yekugadzirira maitiro inoda kufunga nezvezvinhu zvinoverengeka:

Ongorora Chemistry: Kunzwisisa Chirungu, mhirizhonga, uye kugadzikana kwemuongorori kwakakosha.

Sampule vhoriyamu uye kuoma: Iyo vhoriyamu inopesana uye kuoma kweiyo sampule matrix kunogona kuraira kuti ndedzipi nzira dzinogona kuitika.

Sensitivity Zvinodiwa: Kushandiswa kwakasiyana kunogona kuve nekusiyana kwekunzwa kunoda kuti sarudzo yekumanikidza.

Laboratori Zviwanikwa: Kuwanika kwemidziyo yemidziyo kana bhuku rekugadzirisa kunogona kukanganisa sarudzo yehunyanzvi.

Zviitiko Zvakanakisa Zvekugadzirira Kuenzanisa Kugadzirira

Kuti uwane mhedzisiro yakakwana muLC-ms kuongorora, kuomerera kune miitiro yakanaka kwazvo panguva yekuenzanisa kwakakosha:

Kutungamirwa: Gadzira yakaenzana protocol kuti ive nechokwadi chekuenderana nekuongorora.

Kubvumidzwa: Nguva zhinji kusimbisa nzira kuti dzisimbise kuvimbika kwavo uye chokwadi.

Regedza Sampula Kubata: Deredza huwandu hwekunyengedza kudzivirira kurasikirwa kana kusvibiswa.

Shandisa mhando reagents: shandisa marefu-mhando regents kuti urege kuunza kusvibiswa mumasampuli.

Gwaro Dzepfungwa: Chengetedza zvinyorwa zvakadzama zvemaitiro ese kufambisa dambudziko rekugadzirisa uye nzira yekufungidzira.

Mhedziso

Kugadzirira kunoshanda kwekuenzanisira kwakakosha kune zvakabudirira LC-ms kuongorora. Nekunzwisisa nzira dzakasiyana siyana senge mapfumo, protein line, lle, uye kuderera, vaongorori vanogona kusarudza nzira yakakodzera kwazvo kune yavo chaiyo. Kupfuurirazve, kutevera maitiro akanakisa kuchavandudza hunhu hwe data uye uve nechokwadi chekuvimbika mhedzisiro yezvekushandisa mukutsvaga uye maindasitiri marongero.

Mukupedzisa, hunyanzvi hwekufunga kwekugadzirira hazvigone kungogadzirisa zvekufungidzira zvekuongorora, asi zvakare zvinoita kuti mipiro inofambira mberi munzvimbo dzakadai sekiriniki diagnostics, kukura kwezvinodhaka, uye kutarisa kwezvakatemerwa.

Vhura zvakavanzika zvechromatography vial gadziriro yekugadzirira chaiyo uye yakavimbika kuongororwa mumatanho matanhatu ari nyore.Verenga pane tenzi maitiro ehunyanzvi! Matanho matanhatu kugadzirira chromatography vials yekuongorora

Yapfuura:

LCMS VS GCMS: Ndeipi nzira yakanakira kutsvagisa kwako?

Chii chinonzi toc vial uye kukosha kwayo mu chromatography?