Zvakajairika sampu yekugadzirira maitiro eGC-MS

Gasi chromatography-misa spectrometry (GC-MS) ndeyekuongororwa kwehunyanzvi hwekuongorora kwaishandiswa kuongorora mashimoti ehupfu uye semivolatile. Zvichienderana nemhando yemuenzaniso uye chinangwa chekuongorora, maitiro akasiyana siyana anogona kushandirwa kuti agadzirire muenzaniso. Izvi zvinotevera nzira dzinozivikanwa dzinoshandiswa kugadzirira samples forGC-ms Analysis:

Unoda kuziva zvakawanda nezve mutsauko pakati peLC-ms uye GC-MS, ndapota tarisa chinyorwa ichi:Ndeupi musiyano uripo pakati peLC-ms uye GC-MS?

1. Kugadziriswa kwemvura kugadzirira

DILULItion: Liquid samples inowanzoiswa mune yakaderera yekubika point sol sol, acetone, kana dichlomethane yekuwana kusunganidzwa kweinenge 0.1 kusvika 1 mg \ / ml. Izvi zvinovimbisa kuti sampuli inoenderana neiyo GC system uye inoderedza njodzi yekuvhara iyo inlet.

Filtration: Asati ongororo, sampuli inofanira kutemwa kuti ibvise chero zvimedu zvinogona kukanganisa kuongororwa. A0.22 μm firitainowanzo shandiswa.

Centrifugation: yemasampuli inogona kunge iine solids, centrifugation inogona kubatsira kupatsanura mvura kubva kune chero zvinhu zvisina kunaka usati waendesa kune vial.

2. Solid Solid System

Dissolution: Sampuli Solid inofanira kunyungudika mune yakakodzera yakaderera inovhara point solvent. Wedzera chidiki chidiki (zvimedu zvishoma) zveiyo yakasimba kune vial ye solvent uye inverver kakawanda kuti uve nechokwadi chekuparadza kwakazara.

Derivatization: YeSeMi-Yakaomeswa kana Polar Compounds, dentivatization inogona kunge ichidikanwa kusimudzira kusanyara uye kugadzirisa kusaona kwekunzwa. Izvi zvinosanganisira kumisikidza nemamisikidzo yekugadzirisa iyo ana ana ana kuti ive yakaomesesa kuongororwa kweGC.

3. Musoro kuongorora

Static Musoro: Mune iyi nzira, vial ine chisimbiso ine iyo sampu inoitwa pakugara uchiita tembiricha kuti ibvumidze mishonga isina kujairika kuti ishandise mumusoro pamusoro pemuenzaniso. Kamwe kuenzana kunosvikwa, uyu musoro unogona kuenzaniswa nekuongororwa uchishandisa gasi-rakasimba syringe.

Musoro weDynamic Iyi nzira inowedzera zvakanyanya kunzwa kwekunzwa nekuisa pfungwa dzinoenderana nekuongororwa.

Unoda kuziva zvakawanda nezve nei mhirizi dzakashandiswa mu chromatography? Nei mishoms yemusoro inoshandiswa mu chromatography? 12 angles

4. Kubvisa maitiro

Solid phase nhumbi (Spme): Spme inoshandisa fiber yakafukidzwa nechikamu chekutsamwa kuti utore ana kana yegasi chikamu. Iyi nzira inobvumira kutungamira sampling isina kudiwa kwemitambo uye inonyanya kubatsira kune madhiri echokwadi.

Liquid-mvura yekubvisa

5. Matipi ekutarisa

Nitrogen Inchazunza: Iyi nzira inoshandiswa kuisa samples nekubuda sol sol sol soloms yepasi re nitrogen, iyo inobatsira kuderedza sampule vhoriyamu apo kuchengetedza бxer.

Muenzaniso Kugadzirira Kufungisisa

Ita shuwa kuti midziyo yese inoshandiswa ndeye inoenderana uye yakakodzera GC-ms; mvura uye nonvolilile soles inofanira kudzivirirwa.

Sampuli haifanire kunge iine chero yakasimba acids, zvigadziko, mapundu, kana zvimwe zvinosvibisa izvo zvinogona kukuvadza iyo gc mutsara kana kukanganisa nekuongororwa.

Final samples dzinofanira kunge dzakasununguka dzezvikamu uye zviri pachena zvakagadzirirwa mukatiGirazi Vals kudzivirira kusimuka kwezvinhu kubva papurasitiki.

Kuti uwane rumwe ruzivo nezve autosamupler vials yegesi chromatography, kureva chinyorwa ichi: 2 ml autosamppler vials yegesi chromatography

Mhedziso

Kubudirira Kwekutanga Kugadzirira Maitiro akakosha kuti akabudirira GC-ms ongororo. Imwe neimwe nzira ine zvakanakira uye zvine zviyo zvekushandisa zvichienderana nechimiro chemuenzaniso uye chinangwa chakanangana. Nekushandisa idzi nzira idzi, vaongorori vanogona kuvandudza kurongeka, kunzwa, uye kubereka kuwana data ravo rakavimbika munzvimbo dzakasiyana-siyana dzekutarisa, kuchengetedzeka kwechikafu, uye mishonga yekudya.