Gasi Chromatography-Mass SpectRometry (GC-MS) uye Yakanyanya Kuita Liquid chromatography Imwe neimwe nzira ine zvayo zvakanakira uye zvakakodzera kune dzakasiyana mhando dzekuongorora. Kunzwisisa mutsauko wakakosha pakati peGC-MS uye HPLC kwakakosha pakusarudza maitiro ekurudyi zvichienderana nemhando yeiyo sampuli uye chaiwo anoongorora zvinodiwa.

Unoda kuziva mhinduro makumi mashanu nezve HPLC vials, ndapota tarisa chinyorwa ichi:50 Mibvunzo inowanzo bvunzwa paHPLC vials

Inosiyana misiyano pakati peGC-MS uye HPLC

1. Nhare

Musiyano mukuru pakati peGC-MS uye HPLC ndiyo nhare yenhare. GC -MS inoshandisa gase ine mbozhanje, kazhinji inert gasi senge helium kana nitrogen, kutakura iyo yakasarudzika sampuro kuburikidza ne chromatographic column. Izvi zvinoita kuti GC-MS kunyanya yakakodzera kuongorora mishonga inosiririsa inobuda pachena pakukwirira kwakanyanya.

Mukupesana, HPLC inoshandisa iyo Liquid Mobile chikamu, kazhinji musanganiswa wechigadziro akagadziridzwa kune iyo polarity uye solubility yemuenzaniso. Izvi zvinoita kuti Hplc kuongorora huwandu hwakawandisa hwemakomputa, kusanganisira zvese zvisingaenzaniswi uye zvisingaiti zvinhu zvisingaite.

2. Rudzi rweMhando

Mhando dzemasampuli dzinogona kuongororwa nehunyanzvi hwega hwega nzira dzakasiyana siyana. GC-MS inonyatsokodzera kuongorora zvisirizvo kana hafu-isina kujairika organic comparics, senge hydrocarbons, maoiri akakosha, uye zvakasvibiswa zvakatipoteredza. Izvo zvisinganyatsoita zvekupisa-libile kana isiri-isina mishonga. HPLC, kune rumwe rutivi, inogona kubata huwandu hwakawandisa hwemasampuli, kusanganisira polar compound, biomilecules, mishonga yemishonga, uye musanganiswa wakaomarara izvo zvinogona kunge zvine saltts kana mhuka dzakapihwa. Izvi zvinowananisa zvinogadzira huppl yechinyakare sarudzo muminda yakadai seBiochemistry uye mishonga yemishonga.

Wana kuziva ruzivo rwakazara nezve nzira yekuchenesa iyo chromatography sampulies, ndapota tarisa chinyorwa ichi:Inoshanda! 5 nzira dzekuchenesa chromatography sampuli

3. Tembiricha mamiriro

Tembiricha inoita basa rinokosha mumatanho ese, asi nenzira dzakasiyana. GC-MS inoshanda kune yakawanda tembiricha, kazhinji pakati pe150 ° C uye 300 ° C, kuve nechokwadi chekubereka kwemuenzaniso. Iyi yakakwira tembiricha inobvumirwa yekukurumidza kuongororwa, asi inogumisa mhando dzemasampuli dzinogona kuongororwa, sekupisa-kupisa makomputa kunogona kushatisa. Mukupesana, HPLC inowanzoitwa paAmberient kana kusimudzwa tembiricha, ichiita kuti iite kuti iongorore kuongorora kupisa-njodzi pasina njodzi yekuora.

4. Kuparadzanisa Mechanism

GC-MS uye HPLC ine akasiyana kupatsanurwa maitiro nekuda kweiyo mbozhanhare mbozha. MuGC-MS, kupatsanurana kunobva kunyanya pane kujairika kwemakomisheni; Zvishoma zvisingaiti mishonga inowirirana neyekutamba chikamu uye neshungu zvakanyanya zvishoma kupfuura mamwe mishonga inosarudzika.

Mukupesana, Hplc anoparadzanisa makomputa kubva pane zvavanodyidzana neye mobile uye nzvimbo dzekumira, dzinotarisirwa nezvinhu zvakadai sekurapa uye solubility. Polar Compound kazhinji inofamba nepakati nepakati nekukurumidza nekuti ivo vanonyanya kukwezva kuDeal Phose.

5. Nzira dzekutarisa

Nzira dzekuona dzakashandiswa neGC-MS uye HPLC zvakare dzakasiyana chaizvo. GC -MS inosanganisa gasi chromatography ine misa spectrometry, inobvumira zvakanyanya kuongororwa uye kuzivikanwa kwemakomputa zvichibva pane yavo yakawanda-kune yekubhadhara chiyero chevanhu mushure mekuparadzaniswa. Iyi yekubatanidza inopa yakadzama ruzivo rwechimiro nezve fungidziro. Mukupesana ne,HplcKazhinji inoshandisa UV-inoonekwa spectrophotsetry kana index index yekuona, ndeipi matanho ekuti sampuli inotora mwenje kana shanduko yechiedza painopfuura nepakati nepakati. Nepo nzira idzi dzinoshanda kune akawanda mashandisiro ekushandisa, anogona kupa ruzivo rwacho rusina kugadzirwa kupfuura misa misa spectrometry.

6. Zvishandiso uye mutengo kufunga

Zvishandiso zvinodiwa kuti GC-MS uye HPLC zvakare inosiyana kwazvo maererano nekuomarara uye mutengo. GC Systems kazhinji iri nyore; Ivo vanoda gasi rekupa gasi (rinotakura gasi) asi kwete pombi yakakwirira-yekumanikidza nekuti magasi ane kuderera viscosity pane zvinwiwa. Izvi zvinowanzoita kuti GC Systems isingadhuri kushanda kuti ishandire munguva refu. Mukupesana, HPLC Systems inoda pombi yakakwirira-yekumanikidza kusundira solvent solvent kuburikidza nekheni yakazadzwa nechikamu chechiteshi, uye zvakanyanyisa kuti ugare uchikonzerwa nekudiwa kweiyo soldiuses sol sol.

Kusarudza pakati peGC-MS uye HPLC

Paunenge uchisarudza kuti ushandise GC-ms kana Hplc, pane zvinhu zvinoverengeka zvaunofanira kufunga nezvazvo:

Chimiro cheMuenzaniso Wako: Sarudza kana yako sampuli iri yakajeka kana nonvolile.

Kuumbwa kwemari

Inodiwa sensitivity: funga kana iwe uchida ruzivo rwakadzama ruzivo (izvo zvinofarirwa neGC-ms) kana kungoita zvikamu zvekusimbisa (izvo zvinogona kuitwa neHPLC).

Mari inodhura: Ongorora bhajeti rako rekutenga zvekushandisa uye kuchengetedza.

Mukupfupikisa, zvese gc-ms uye HPLC zvakakosha zvishandiso zvekuongorora muongororo, uye nzira imwe neimwe ine zvakanakira zvikumbiro zvakati. Nokunzwisisa misiyano yavo yakakosha (i.e., nhare mbozha, nzira yekuparadza, masayendisiti anogona kuita chisarudzo chekuzivisa kuti tekinoroji yakakwana sei kune zvavanoda zvekuongorora.

Unoda kuziva zvakawanda nezve mutsauko pakati peLC-ms uye GC-MS, ndapota tarisa chinyorwa ichi:Ndeupi musiyano uripo pakati peLC-ms uye GC-MS?

Yapfuura:

Ndeipi mhedzisiro yeiyo vial size pa chromatography mhinduro?

Zvakajairika sampu yekugadzirira maitiro eGC-MS

Matagi: Gasikegraphygraphy chromatography-vigial Chromatography gasi-chromatography-vials HPRwanalysis Hplc-vials

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