Maitiro ekusarudza pakati peHPLC uye GC vials? Mukuenzanisa-kwakadzama kuenzanisa kwezvinhu zvinoenderana

Nhanganyaya

Mu chromatographic ongororo, kusarudza iyo chaiyo sampuli yekuenzanisira yakananga kukanganisa uye kubereka kwemhedzisiro yako. Chinyorwa ichi chinopa kuputsa kwakazara kwemusiyano pakati peHPLC uye GC sampulios, maitiro ekunyora, kuwirirana, kukubatsira kuti uite masangano esainzi uye nekuvandudza mari yekubudirira.

I. Kwakakosha mutsauko pakati peHPLC uye GC vials

1. Chikumbiro Chikumbiro

HPLC vials.

GC vials: Inoshandiswa mugesi chromatography (GC) uye gc-ms

2. Zvinyorwa uye Dhizaini maficha

Vial mitsipa mitsipa:

HPLC:Kazhinji usashandise screw kumusoro kana snap tops, yakanaka yekudzokororwa kuwanikwa uye auto-samplers.

GC:Kazhinji crimp yepamusoro vials ine aluminium caps uye ptfe \ / Silicone Septa yekuisa-tembiricha kuiswa chisimbiso.

Unoda kudzidza nezvenzira shanu dzekuchenesa chromatography sampuli? Dzvanya apa kuti uwane chinyorwa chakadzama

Kusarudzwa kwezvinhu:

HPLC:Kazhinji yakagadzirwa neyakanyanya-yekuchena borosilrate girazi, ine amber shanduro dzechiedza-chinonaka samples.
GC:Inoda yakakwira-tembiricha kuramba-borosilrate girazi rakajairika, ine PFA PLASTILT inoshandiswa mune zvakakosha zvikumbiro.

Ii. Kiyi yekufunga ye chromatography vial zvinhu

1. Girazi zvinhu

Borosilrate Girazi: Zvakanyanya inter uye kupisa kupokana; yakakodzera kune ese HPLC uye GC ongorora.
Amber girazi: Inopa kuchengetedzwa kwechiedza kune mwenje-unonaka samples.

2. Zvinhu zvepurasitiki

Polypropylene (PP): Mutengo-unobudirira uye wakakodzera kushandiswa kwakaderera-tembiricha; kushoma solvent uye kupisa kupisa.
PerfluorokolagoKoxy (PFA): Kukwikwidza kwakanyanya kupokana uye nemakemikari kana Yakanakira kuteedzera uye yakakwirira-tembiricha gC ongorora.

Kuda kuongororachii chinonzi gc musoro? Dzvanya apa kune yakadzika kutarisa

3. Cap uye Sept zvinhu

Ptfe \ / silicone Septa: Dzivirira solvent kupinda muHPLC; Chengetedza chimiro pasi pekupisa muGC.
Kuisimbisa nzira: GC Crimp Caps inoda zvishandiso zvakakosha, nepo Hplc Screw Caps ari nyore kubata.

III. Nyaya dzinoenderana uye maitiro ekuzvidzivirira

1. Chiridzwa chinongedzo

Autosampler Kuenderana: Ita shuwa kukwirira kwe vial uye diameter mechi tray tsanangudzo (i.e., 2 ml standard vials).
Septa puncture kuramba.

Kutarisana neVial Caps uye Septa, iwe unofanirwa kuziva Cleak pano

2. Kusvibisa njodzi kutonga

Kudzivisa kubvisa: Kune yakakwira-tembiricha GC, sarudza inert Glass kana PFA vials.
Kudzivirira Kuyambuka-Kusvibiswa: Shandisa Certified Pre-Yakacheneswa Vials (i.e., USP kirasi a).

Iv. Kutenga Chiratidziro: Kusarudza iyo chaiyo vial yeHplc uye GC

Tsanangura zvido zvekuedza: Funga nezve tembiricha tembiricha, sampulialility \ / Chiedza-kunzwa, uye jekiseni yekuwanda.
Batanidzai sosi yemhando: Ita simbisa chaiyo mutsipa mhando, vhoriyamu, uye autosampler yakafanana.

Zvichine chokwadiCrimp vial vs snap vial vs screw cap vial, maitiro ekusarudza? Tenzi the Section Logic pano

Reva zviyero zveindasitiri: Shandisa vials ne USP kana iso certification yemhando yekuenderana.
Mutengo-wekubatsira bvunzo: Yekuedza kazhinji, sarudza inokwanisika girazi. yekuongorora kwekuongorora, sarudza inert midziyo.

V. FAQ - Mibvunzo yakajairika Inopindurwa

Q: HVLC vials vials kushandiswa kuongorora kweGC?
A: Kana yakagadzirwa nehurongwa hwebambo uye kupisa unodzivirira (i.e., kusvika 400 ° C), vanogona kushandiswa kwenguva pfupi. Nekudaro, kushandiswa kwenguva refu pasi pekupisa kwakanyanya hakukurudzirwe.

Q: Sei kudzivirira kusvibiswa kwevanhu?
A: Sarudza Certified Pre-Yakacheneswa Viums kana yakachenesa ivo vekutanga vachishandisa chromatographic-giredhi sol.

Q: Varume vepamusoro-pamusoro vevakamuri vanodiwa kuti GC?
A: Crimp vials inopa kuiswa kuri kuiswa kuri nani pakupisa kwakakwirira, kunyangwe zvimwe zviridzwa zvinotsigira zvibodzwa zvechikwama zvine maAcapter.

Kuda kuzivaMaitiro Ekuchenesa Miromo Chromatography Val? Dzvanya apa kuti uongorore mberi

Mhedziso

Kusarudza kodzero yeHPLC kana GC sampuli inoda kufungidzirwa kwemamiriro ekuedza, chiridzwa chakaenzana, uye maitiro ekuenzanisira. Nezvishandiso zvekutarisa uye zvakatemwa, iwe unogona kuwedzera kuvimbika kwedata yekuvimbika uye kuderedza mari yakazara yekuedza.