Nei HPPLC iri kutsoropodzwa mukuongororwa kwezvakatipoteredza

Yakakura Performance Liquid chromatography (HPLC) ndiyo yakakosha yekuongorora maitiro ayo anoshandiswa zvakanyanya mukuongororwa kwezvakatipoteredza kuti aone, uye kuongorora zvisina kujairika mumvura, ivhu, uye mhepo. Sezvinonetsekana nezvemamiriro ekunze zvinokonzerwa nemabasa endarama, maguta, uye zviitiko zvekurima zvinokura, HPLC zvave zvakakosha kuti uchengete kuchengetedzeka kwezvakatipoteredza uye nekuteerera nezvematongerwo enyika.

HPLC ndeye maitiro akaomarara anoparadzanisa zvikamu mumusanganiswa zvichienderana nekudyidzana kwavo neyekufambisa uye nhare mbozha. HPLC inowana kukwidziridzwa uye kunzwa nekushandisa kudzvinyirira kumanikidza sampuli yemvura kuburikidza nekhoramu yakazadzwa neyekutamba zvinhu. Ichi chimiro chinogona kuona trace huwandu hwevasvibisi izvo zvingave zvisingaonekwe nenzira dzisina kudzikama.

Unoda kuziva zvakawanda nezveHPLC Sampula, ndapota tarisa chinyorwa ichi:HPLC yemuenzaniso yekugadzirira mhinduro dzemhedzisiro yakanaka

Mashandisirwo makuru eHPLC mukuongororwa kwezvakatipoteredza

1. Kuongororwa kwemvura

Imwe yeiyo huru kushandiswa kweHPLC mukuongororwa kwezvakatipoteredza ndeyekuongororwa kwemvura. Zvinosvibisa simbi dzinorema, mishonga yekuuraya zvipuka, mishonga yemishonga, uye inokanganisa kukanganisa makemikari kunogona kuisa njodzi dzinokosha kuhutano hwevanhu uye aquatic ecosystems. HPLC inobvumira iwe ku:

Kusvibisa kuona: HPLC kunogona kuziva uye kujekesa zvinhu zvine ngozi pazvinhu zvakaderera kwazvo mumasampula emvura. Semuenzaniso, mishonga yekuuraya zvipuka zvinowanzowanikwa mukurima runoffff inogona kuonwa.

Kuteedzera kuongorora: masangano ekuzvidzora anoda kujaira kuongorora kwemiviri yemvura kuve nechokwadi chekutevedzera zviyero zvekuchengetedza. HPLC inopa yakavimbika data iyo inobatsira zviremera kutora matanho anodiwa ekuchengetedza hutano hweveruzhinji.

2. Ivhi inosvibisa kuongorora

Ivhu ndechimwezve madrix yekuongorora kwezvakatipoteredza nekuti inogona kuunganidza akasiyana-siyana ekusvibisa anogadzirwa nemaitiro ekurima, zviitiko zveindasitire, uye kurasa marara. Hplc inoita basa rakakosha mune inotevera:

Kuzivikanwa kwePellutants: HPLC inobatsira kuongorora huwandu hwekusvibiswa nekuongorora zvinhu zvakadai sePolyclic tomiccarbons (PAHS), PCBS), uye mishonga yekuuraya zvipuka mumapuranga ivhu.

Remediation matanho kuongororwa: MuBiosededation zvirongwa zvakanatswa pakuchenesa nzvimbo dzakasvibiswa, HPLC dzinogona kuongorora kushanda kwekurapa nzira dzekurapa nekunyora kusvibiswa kwevasvibisi nekufamba kwenguva.

3. Mhando yemhando yekutarisa

HPLC inoshandiswawo kuongorora mhepo pllutants uye tarisa yemhepo mhando. Zvikumbiro zvinosanganisira:

Vadivelu Comedy Command Compound (VOC) Kuedza: Vocs vane ngozi makemikari anoburitswa kubva kune akasiyana masosi, kusanganisira pendi, uye maitiro ekumaindasitiri. HPLC inogona kupatsanurwa uye inokonzeresa makomputa aya mumatanho ekumhepo.

Kuongorora kweChisikoko: Iyi nzira inobatsira kuona zvinokuvadza zvimedu zvinogona kukanganisa hutano hwekufema uye zvinobatsira pakusvibiswa kwemhepo.

4. Kuongorora marara

Chimiro chetsvina chakakosha kune maitiro akakodzera uye ekudzokorora maitiro. HPLC inogona kukubatsira:

Ongorora marara marara: HPLC inobatsira kuona yakakodzera kurasa nzira nekuzivisa zvinhu zvine njodzi mumarara.

Ita shuwa wakachengeteka.

Unoda kuziva zvakawanda nezvezvinoshandiswa zve chromatography vials, ndapota tarisa chinyorwa ichi: 15 zvikumbiro zve chromatography vials muminda dzakasiyana

Zvakanakira kushandisa HPLC yekuongororwa kwezvakatipoteredza

Pane zvakanakira zvakati wandei kushandisa HPLC mukuongororwa kwezvakatipoteredza.

1. Kukwikwidzana kwakanyanya uye kunyatsojeka

HPLC inogona kuona trace mazinga ekusvibisa, achiita chishandiso chine simba chekuongorora pllutants iyo inogona kunge ine zvakakomba zvisikwa kana hutano mhedzisiro. Uku kunzwa kwepamusoro kunobvumidza kwekutanga kuwanikwa kwengozi dzinogona kuitika vasati vakudziridza kuva nematambudziko akakura.

2. Kuedza

Hplc yave chishandiso chesimba kune masayendisiti ezvematongerwo enyika nekuti inogona kugamuchira akasiyana okuongororwa mumatrices akasiyana (mvura, ivhu, mhepo, mhepo). Mhando dzakasiyana dzekuongorora (UV-vis-vis, fluorescence, misa spectrometry, etc.) inogona kushandiswa zvichienderana nezvinodiwa zvekuongorora.

3. Robustness

Kubata kweHPLC masisitimu eHPLC kunoita kuti vatore mataira akaomesesa anowanzo kuwanikwa mumamiriro ekunze. Maitiro akakodzera ekugadzirira maitiro, akadai seyakagadziriswa chikamu chekuratidzira

Unoda kuziva zvakawanda nezve chromatography vials yekuongorora kwemvura, ndapota tarisa chinyorwa ichi: Chromatography vials yemvura yekuongorora

Yakakwirira-perform yemvura chromatography inomira sechinhu chakajairwa kuongorora kwezvakatemerwa nekuongororwa kwezvakatipoteredza nekuda kwekunzwa kwekunzwa, kuwanda, uye kusimba. Zvinoshanda zvinopindirana nemhirizhonga yemhando yemvura, ivhu rekuongorora kwemweya, marara emhando, uye marara ezvematambudziko - mumwe nemumwe anopa zvakanyanya kunzwisisa kwehutano hwezvakatipoteredza nematambudziko ehutano. Sezvo ruzivo rwepasi rose pamusoro pekusvibisa zvinowedzera pamwe nekudzokorora zvinodiwa nenzvimbo dzakachenesa, basa reHplc richaramba richikura mukuedza kutsigira hutano hweveruzhinji uye kuchengetedza zvakasikwa zvizvarwa zvinotevera.