Sampula Kugadzirira & Filtration: Kufunga kunaka mu chromatography

Sample chaiyo midziyo yakachena-yakachena, bvisa kuongorora, Enrich Ongorora, uye inodiwa-inobvisa manyorerwe analine Kukundikana pano zvinoguma nemumuswe wepamusoro, kudhonzwa zvidhori, kana column block.

1. Kusvibisa & matrix kupindira

Dambudziko

• Baseline Drift uye Kukwira Ruzha Kucherekedza Minimum Peaks.

• Peaks isingatarisirwi inoonekwa kubva kusvibiswa kusviba.

• Kubereka kwakashata pakati pemasangano.
  

  

Chikonzero

• Vakasara muGlassware: isina kukwana acid-kushambidza mashizha akasviba; Plasticware inogona leach mapurasitiki.

• Kuchenesa vamiririri: Trace guva muRinse soles inoramba iri mudumbu.

• Zvemamiriro ekunze
  

  

Mhinduro

1. Somed Skying SOP. yakazvipira kudzikisira kwemapurasitiki.

2. Pre-Filt.

3. Nzvimbo Dzakadzora: Ita prep mumarara-inoyerera hood kuderedza mhepoborne kusvibiswa.
   
   

2. Filter kuvhara kubva kune vasingawirirani mafirita

Dambudziko

• Autosamupleur yekumanikidza maalarm uye anowanzo tsiva.

• Yakaderera kuyerera chiyero, zvisingapindirani nzvimbo dzepamusoro.

• Yakakwirira backpressure uye column inlet block.
  

  

Chikonzero

• Membrane Mismatch: Nylon inozvimba mu organic sol; PVDF inokambaira pakukwidziridzwa kupisa.

• Size isiriyo yePore: 0.22QM mafirita anoteya zvinhu zvakanaka asi kuderedza kuyerera; 0.45QM mafirita anogona kunge achiri kufukidza nemacrocrysts.

Mhinduro

1. Membrane-solvent matrix. Dzivisa cellulose acetate mune yakasimba acids.

2. Sarudza yakakodzera pore saizi. Prefilter yakakwira-viscosity samples kuburikidza ne5μm.

3. Monitor δp: Track yekumanikidza kusimuka; Tsiva firita kana backpressure inowedzera> 15%.
   
   

   

3. Solvent-membrane makemikari kusakwanisa kusakwanisa

Dambudziko

• Yakasarudzika sampuli inooneka ine makore kana inotakura zvidimbu.

• New Baseline ruzha kana ghost peaks post-filtration.

• Kuderedza anaClowte kupora.
  

  

Chikonzero

• Solvent kuzvimba: thf inopisa ptfe, inoshandura pore saizi uye kuyerera.

• Membrane Leachable: Nylon muForm Casid inoburitsa Oligomers iyo inotora na210nm.

Mhinduro

1. Kuenzanisa Kwekuenderana: Soak firita mune yakagadzirirwa solvent ye 240 pa 25 c; kuongorora kwekuzvimba kana kuchinjika uremu.

2. Pilot LC-ms bvunzo: Enzanisa yakazara ion chromatograms yeiyo yakasviba vs. isina kubikwa sampuli.

3. Shandisa yakaderera-leach filters: Ultrasonic-Welded PTFE kana PES Pasina MainhesOres.
   
   

Mhando system: kudzivirirwa-kutarisa-kururamisa

Kudzivirira

Gadzirisa masheya ekuchenesa, kupera, uye musarudzo membrane kuti udzivise zvikanganiso.

Kuongorora

Rekodha kuyerera kuyerera, kudzoka, uye isina-sampula mabhasiketi kuti uone kudhirawa.

Kururamisa

Chengetedza kuongororwa kwemuti wekutadza kwekukurumidza kudzima midzi-kukonzera kuzivikanwa uye kururamisa chiito.

"Ongororo yekuongorora inotanga nekudzikisira chidiki chidiki."