Unoziva nezve sielization kurapwa kweHPLC vial?

Silanation yeHPLC vialsChiitiko chakanyanya chakagadzirirwa kugadzirisa chromatographic performance nekuderedza kuongorora kwekuongorora kune vial girazi. Uku kurapwa kunonyanya kubatsira pakuongororwa kwehuwandu Chinyorwa ichi chinopa kutarisisa kwakazara kwechiitiko chekufunga, zvakanakira, zvinoshandiswa, uye kufunga kwekushandisa mukuita kwakakura kweChromatography (HPLC).

Chii chinonzi sielization?

Sielization inosanganisira kushandisa silane yakavezwa kune iyo yemukati nzvimbo yemapurastili yegirorti. Kuita uku kunoita zvinobudirira deactivates yemahara hydroxyl (silanol) mapoka pagirazi pamusoro, inozivikanwa kuti ifambe nemhando dzakasiyana-siyana dzekuongorora. Nekusasking idzi nzvimbo dzinoenderana, sielization zvakanyanya inoderedza adsorption yekuongorora kumadziro emidziyo, nokudaro ichiratidza kuti udzoreke nekudzokorora uye zvimwe zvakanyatsoongorora zviwanikwa. MuHPLC vials, kurapwa uku kwakanyanya kunongedza silanol (si-oh) mapoka pane iyo mamiriro emukati yeiyo borosilrate girazi. Aya maHydroxyl mapoka anogona kupindirana nePolar ongororo, anotungamira kumubatsiri adsorption uye kurasikirwa panguva yekuongorora.

Sielization Process

Iyo sielidaization maitiro kazhinji inosanganisira matanho anotevera:

Kuchenesa: Kutanga, kuchenesa girazi pamusoro penyaya kunyatso shandisa sol sol sol sol sol so acetone kuti ubvise chero zvinangwa zvinogona kukanganisa slanization.

Kushandiswa kweSilane: Iyo methyl siligizing mumiririri yakaunzwa pane iyo yemukati yeiyo vial neVapor deposition. Mumiririri anoita nehydroxyl mapoka kuti aumbe covaltal yakasungwa hydrophobic layer isingashande uye isingasviki adsortive.

Kuvhara kweCovent: Silane Inoita neHydroxyl Mapoka pagirazi pamusoro peHydrophobic layer

Kuporesa: Mabhodhoro ehondo anowanzo kurapwa kuti ave nechokwadi chekuti silane inoumba chisungo chakagadzikana kune iyo girazi kumusoro, nekukasira kusimba kwemamiriro ezvinhu uye mamwe mamiriro akasangana panguva ye chromatography.

Mhedzisiro yacho inguva ine yakagadziridzwa iyo inogadziridzwa kufambidzana nehupombwe compounds, kuvandudza sampula kupora uye kuongororwa kwekuongorora.

Zvakanakira Silanation

Kushandisa Sielided HPLC vials inopa zvakakwanisika zvakanakira:

1. Kuvandudza Muenzaniso Kupora

Sielaned vials inogona kuderedza zvakanyanya kuderedza kwekuongorora kumadziro emasikati. Izvi zvinonyanya kukosha kuti uwedzere kuongorora kuongorora, uko kurasikirwa kushoma kwekuenzanisira kunogona kukanganisa huwandu hwehuwandu. Nekudzivirira adsorption, sielid vials inoona kupora kwakanyanya kwekuongorora.

2. Kuvandudza chokwadi

Uku kurapwa kunoona kuti mhedzisiro haina kukanganiswa nemumiriri wenguva yakasara kuomerera kune iyo vial nzvimbo, nekuvandudza kurongeka kwehuwandu iyo ndeyekutsoropodzwa kwehuwandu hwenzvimbo iyo chaiyo yekunyora.

3. Kuenderana neUne Various Ongorora

Sielied vials yakakodzera kune dzakasiyana-siyana mafaro, kusanganisira izvo zvinosanganisira polar uye hydrophobic compounds. Ivo vanonyanya kukodzera kune mishonga yemishonga, biopolymers uye zvimwe zvinhu zvine tsvarakadenga izvo zvinogona kuwirirana neyakajairika girazi nzvimbo.

4. Hupenyu hurefu

Iyo vapor phase sielization maitiro anogadzira machira akaomesesa anochengetedza zvivakwa zvake nekufamba kwenguva. Sielized Coatings inoramba yakagadzikana kwenguva yakareba kunyange painoratidzwa kune dzakasiyana-siyana soles uye dzakakodzera kuchengetedza kwenguva refu.

Zvikumbiro zveSilaned HPLC vials

Sielaned HPLC vials inogona kushandiswa muminda dzakasiyana siyana:

1. Kuongorora kwezvinodhaka

Mumapakero emishonga, makomendi eSielized anoshandiswa:

Kugadziriswa kwekutarisa: Kuverengera kuti kuumbwa kwezvinodhaka kunoramba kuchengetwa panguva yekuchengetedza uye kuongororwa.

Kuongorora kwehuwandu

2. Kuedzwa kwezvakatipoteredza

Aya mairi akakosha kuti aongororwe zvekare:

Mvura mhando yekuyedza: Ongorora samples dzemvura kune zvinoshatisa uchirambidza kurasikirwa kwekushambadzira.

Ivhu rinobvisa kuongorora: Gadzirisa ivhu rinobuda kune mamwe makemikari ongororo pasina kurasikirwa neyechinangwa chekuongorora.

3. Kudya kwekuchengetedza kwekuchengetedza

Mune zvekuchengetedza zvekuchengetedza zvekushandisa:

Pesticicide yakasara kuongororwa: Simbisa kunyatso kuwanikwa kwemazinga ezvipenga muchikafu nekudzivirira kurasikirwa kwemuenzaniso panguva yekuongorora maitiro.

Ongororo yemari yekudyara: Ongorora samples dzekudya kune zvinovaka zvemukati pasina kupindira kubva kune vial kutaurirana.

4. Kutsvaga uye Kubudirira

Mune mamiriro ekutsvagisa:

Sampula Kugadzirira: Inopa yakavimbika yekuchengetedza mhinduro yemhando dzakasiyana siyana samples isati ongorora.

Kuedza kwayedza: Kufambisa maedzo kunosanganisira zvinosanganisira mishonga inotaridzika inoda inert mamiriro.

Precution paunenge uchishandisa Sielized vials

Nepo Sielized vials inopa zvakawanda zvakanakira, pane zvimwe zvakakosha zvekuona:

1. Sarudza iyo chaiyo sampuli yemhando yebotle

Izvo zvakakosha kuti usarudze mhando chaiyo ye sialized vial kune yako chaiyo application zvinodiwa. Zvinhu zvakadai sekuenzanisira vhoriyamu, kumakemikari kuenderana, uye kusuwa kwekunzwa kunofanirwa kutungamira kusarudzwa.

2. Kuchinjira kwekushandisa

Kurapa kwakakodzera kwakakosha kuchengetedza kutendeka kweiyo silanised pamusoro:

Dzivisa kuonana zvakananga neyakagadzirirwa nzvimbo kudzivirira kusvibiswa.

Pfeka magurovhosi akachena paunenge uchibata vials kuti uderedze kuchinjiswa kwezvinhu zvasara.

3. Kuenderana ne solvent

Nepo Sielided maficha anowanzo pokana kune akawanda masosi ekunyora, zvakakosha kuti uone kuenderana nemakemikari chaiwo anoshandiswa mukuongororwa kuti adzivise kusvibiswa kana kurasikirwa kwekushanda.

Zvimwe Zvekurapa

Nepo Sielization Inoshanda, pane zvimwe zvingasarudzwa senge zvakaderedzwa sosi yezviitiko (RSA) vials. Dzima dzakasikwa dzakagadzirirwa kuve neyakajeka nzvimbo yepasi pebasa uye usavimba neSilane machira. Iyi nzira inogona kupa zvizere mhedzisiro yezvimwe zvinoshandiswa zvekushandisa izvo silanation yetsika inogona kutadza nekuda kwekuvhara isina kukwana kana kusvibiswa nekufamba kwenguva.

Kana iwe uchida kuziva zvakawanda nezve siliconized hplc vials, ndapota tinya chinyorwa ichi:"Mharidzo kune siliconized hplc vials"

Silanzation yeHPLC vials chiitiko chakakosha chinovandudza maitiro ekuongorora nekudzora analyte kunamatira nekuwedzera sampula. Nekugadzirisa mamiriro emukati memukati megirorti egirosi, kurapwa uku kunoita kuti zviitike zvakaringana, zvakavimbika muzvinyorwa zvakasiyana siyana mumishonga yekuedzwa, kuchengetedza kwekudya, uye marongero ekutsvagisa. Nokunzwisisa zvakanakira uye kunyatsoshandiswa kurongeka zvakabatana nevarume veSilaned, Laboratori vanokwanisa kunyatsoongorora maongororo yavo yekuongorora vachiramba vachichengetedza mhando yepamusoro uye chaiyo miitiro yemigumisiro. Sezvo tekinoroji yeSielization ichiramba ichikwira, vatsvagiri vanogona kutarisira kuvandudzwa kwekuita vial mashandiro uye kushanda kwemhepo dzakasiyana siyana dzesainzi dzidziso.