Iwe unoziva nezve pepa chromatography?

Pepa Chromatography inoshandiswa kwazvo maitiro anoparadzanisa uye anozivisa musanganiswa wezvinhu zvakavakirwa pane yavo yekubatana kweiyo nzvimbo yekunongedza (pepa) uye solvent). Izvo zvinonyanya kubatsira mumakemikari uye biochemistry yekuongorora zvidiki zvidiki zvezvinhu zvakadai sekuti pigment, amino acids, uye mamwe ma compound.

Chii chinonzi papepa chromatography?

Pepa Chromatography rudzi rwemvura chromatography umo chikamu chomukati chipenga chepafa uye nhare mbozha ndiyo solvent inoyerera nepepa refaira. Maitiro acho anovimba nekuparadzaniswa kwehuwandu hwemakomputa pakati peiyo stationary chikamu (pepa refaira) uye nhare yefoni (solvent). Sezvo solvent inokwira pepa refaira neCapilly chiito, inotakura zvikamu zvemusanganiswa pamari dzakasiyana zvichienderana nehukuru hwavo hwenyika.

Nheyo dzekutanga dziri kumashure kwepepa chromato dzazvinogona kunzwisiswa kuburikidza nepfungwa mbiri huru: Parkitio chromato uye adsorption chromatography.

Parkition Chromatography: Mune iyi mhando, zvinhu zvinogoverwa pakati pezvikamu zviviri zvemvura. Chikamu chekumira chine mvura mumapepa epepa refirita, nepo nhare pendi ndiyo iyo solvent iyo inofamba kuburikidza ne pores idzi. Kupatsanurwa kunoitika nekuti makomputa akasiyana ane hukama hwakasiyana kune iyo stationary uye nhare mbozhanhare.

Adsorption chromatography: Pano, iyo yakasimba nzvimbo yepepa inoita seyekumira chikamu uye iyo yemvura solvent zviito sezvinoita nhare. Izvo zvikamu mumusanganiswa zvinoparadzaniswa zvichienderana neyemambadziro yavo kune iyo yakasimba nzvimbo uye yavo solubility muchikamu chemvura.

Zvikamu

Chikamu chesimba: chromatographic bepa, kazhinji yakagadzirwa nemheremalose. Iyo inopa pamusoro pezvikamu zvekutevedzera.

Nhare mbozha: iyo solvent kana musanganiswa we solvent inofamba pabepa necopilly chiito. Sarudzo yeSolvent inogona kukanganisa zvakanyanya maitiro ekuparadzanisa.

SAMPLE: Iyo musanganiswa kuti uongororwe, kazhinji yakaonekwa pane yakatarwa yekutanga pamba papepa.

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Pepa chromatography maitiro

Kugadzirira: Sarudza pepa remhando yepamusoro ine imwe porosity yekuve nechokwadi chekuparadzaniswa kwakanaka. Nyorera chikamu chidiki chemusanganiswa wemuenzaniso kune bhasikoro re chromatography pepa.

Sampula Kugadzirira: Nyura musanganiswa wemuenzaniso mune yakakodzera solinen sosi yechikamu chenhare.

Spotting: Shandisa capillary kana micropipette kudonhedza chidiki chidiki chemutemo mhinduro pane iyo basterine pabepa refaira.

Development: DZVANYA PASI PASI PASI PEDZISIRA PEPI KUTI UCHIDZIDZO SOLVERS Solvent Sezvo chiitiko che capillary chinoitika, iyo solvent inosimuka kuburikidza nebepa rekutidza, rakatakura zvikamu zvekuenzanisira nazvo.

Kuparadzanisa: Sezvo iyo solvent inofamba, zvikamu zvakasiyana zvemuenzaniso zvinofamba pamitero yakasiyana nekuda kwekudyidzana kwavo neiyo nzvimbo yekufambisa uye yakasiyana solubility chikamu.

Kuona Izvo zvikamu zvinogona kuoneka uchishandisa UV mwenje, makemikari reagents, kana nekuona shanduko yemavara.

Kuongorora: Iyo chinhambwe chakafamba nechikamu chimwe nechimwe chine chekuita neiyo solvent kumberi kunoyerwa kuti uverengere kukosha kweRF (Commention Factor) yekuzivikanwa.

Zvikumbiro zvepepa chromatography

Pepa Chromatography ine huwandu hwakawanda hwekushandisa muminda dzakasiyana siyana:

Biochemistry: Kuongororwa kweAmino Acids, mashuma, uye mamwe ma biobolecules.

Mishonga: Kuzivikanwa kwezvinhu zvinoshanda uye kusvibiswa mune zvinogadzirwa zvinodhaka. Yaishandiswa kuratidza makomendi anoshanda uye kusvibiswa mune zvinogadzirwa zvinodhaka panguva yekuvandudza uye mhando yekudzora.

Sciental Science: Pepa Chromatography inoshandiswa kuona zvinosemesa mumasampuli emvura uye ongorora zvinosvibisa mune ivhu dzinobuda.

Indasitiri yeChikafu: Maitiro ekugadzira anobatsira pakuongorora zvinowedzera, zvinosvibisa, uye vota muchikafu kuti vave nechokwadi chekuteerera nemirairo yekuchengetedza.

Zvakanakira zvePepa Chromatography

Mutengo Kubudirira: Zvinoda michina miviri uye zvigadzirwa zvichienzaniswa nedzimwe nzira dzechromatographices dzakadai seHPLC (High performation Liquid chromatography). Kujekesa: Maitiro acho ari nyore uye anogona kuitwa nemidziyo yekutanga yemarabhoritari, saka inogona kushandiswa kune zvinangwa zvekudzidzisa.

Low Sampuli Vhoriyamu Inodikanwa: Chete Chikamu cheSampuli chinodikanwa chekuongororwa, zvichiita kuti ive yakakodzera kune yakakosha kana mashoma samples.

Kuisana

Kugumira kwepepa chromatography

Zvisinei nezvakanakira, pepa chromatography ine zvimwe zvevengo:

Yakakwana simba: Kazhinji yakakodzera kune madiki ekuenzanisira mavhoriyamu.

Chisarudzo: Kugadziriswa kunogona kunge kusiri kwakakwirira sezvakawanikwa neyakawandisa maitiro akadai seHPLC kana Gasi Chromatography (GC).

Nguva Inonline: Nekuda kweiyo inononoka kutama mwero, maitiro anogona kutora nguva yakareba achienzaniswa neimwe chromatographic nzira.

Kuregererwa kuongororwa kwehuwandu

Pepa Chromatography inosara yakakosha maitiro ekuparadzanisa uye kuongorora musanganiswa muminda yakasiyana siyana yesainzi. Kuve kuri nyore, mutengo-wekubudirira, uye kugona kupa zvinonyadzisa zvinoita chishandiso chakakosha kunezvemakemikari nevatsvagiri. Pasinei nezvinogumira zvichienzaniswa neyakawedzera chromatographic maitiro, ichiri kushandiswa zvakanyanya nekuda kwezvinangwa zvekudzidzisa uye kuongororwa kwekutanga mumarabhoritari.

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